LOCUS       sequence-194170-       10776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10776)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 10776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: 
            pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
FEATURES             Location/Qualifiers
     source          1..10776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          69..296
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      308..317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3059..3079
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3080..3100
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3119..3145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3173..4153,4154..4156,4157..5671)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             5675..5695
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5714..5863
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5891..5944
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5960..6346
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             6401..6421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6437..6979
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             7004..7375
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(7403..7420)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7409..7616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7625..7765
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7625..7754
                     /label=AAV2 ITR
     rep_origin      7840..8295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7927..7946)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8137..8158
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8312..8331)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8431..8453
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8491..8509)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8577..8681
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8682..8750,8751..9542)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8900..8919)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9713..10301
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10202..10221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        10373..10658
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
       61 ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc
      121 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc
      181 cttctcctcc gggctgtaat tagctgagca agaggtaagg gtttaaggga tggttggttg
      241 gtggggtatt aatgtttaat tacctggagc acctgcctga aatcactttt tttcaggttg
      301 gaccggtgcc accatggtca gctactggga caccggggtc ctgctgtgcg cgctgctcag
      361 ctgtctgctt ctcacaggat ctagttcagg ttcaaaatta aaagatcctg aactgagttt
      421 aaaaggcacc cagcacatca tgcaagcagg ccagacactg catctccaat gcagggggga
      481 agcagcccat aaatggtctt tgcctgaaat ggtgagtaag gaaagcgaaa ggctgagcat
      541 aactaaatct gcctgtggaa gaaatggcaa acaattctgc agtactttaa ccttgaacac
      601 agctcaagca aaccacactg gcttctacag ctgcaaatat ctagctgtac ctacttcaaa
      661 gaagaaggaa acagaatctg caatctatat atttattagt gatacaggta gacctttcgt
      721 agagatgtac agtgaaatcc ccgaaattat acacatgact gaaggaaggg agctcgtcat
      781 tccctgccgg gttacgtcac ctaacatcac tgttacttta aaaaagtttc cacttgacac
      841 tttgatccct gatggaaaac gcataatctg ggacagtaga aagggcttca tcatatcaaa
      901 tgcaacgtac aaagaaatag ggcttctgac ctgtgaagca acagtcaatg ggcatttgta
      961 taagacaaac tatctcacac atcgacaaac caatacaatc atagatgtcc aaataagcac
     1021 accacgccca gtcaaattac ttagaggcca tactcttgtt ctcaattgta ctgctaccac
     1081 tcccttgaac acgagagttc aaatgacctg gagttaccct gatgaaaaaa ataagagagc
     1141 ttccgtaagg cgacgaattg accaaagcaa ttcccatgcc aacatattct acagtgttct
     1201 tactattgac aaaatgcaga acaaagacaa aggactttat acttgtcgtg taaggagtgg
     1261 accatcattc aaatctgtta acacctcagt gcatatatat gataaagcat tcatcactgt
     1321 gaaacatcga aaacagcagg tgcttgaaac cgtagctggc aagcggtctt accggctctc
     1381 tatgaaagtg aaggcatttc cctcgccgga agttgtatgg ttaaaagatg ggttacctgc
     1441 gactgagaaa tctgctcgct atttgactcg tggctactcg ttaattatca aggacgtaac
     1501 tgaagaggat gcagggaatt atacaatctt gctgagcata aaacagtcaa atgtgtttaa
     1561 aaacctcact gccactctaa ttgtcaatgt gaaaccccag atttacgaaa aggctgtgtc
     1621 atcgtttcca gacccggctc tctacccact gggcagcaga caaatcctga cttgtaccgc
     1681 atatggtatc cctcaaccta caatcaagtg gttctggcac ccctgtaacc ataatcattc
     1741 cgaagcaagg tgtgactttt gttccaataa tgaagagtcc tttatcctgg atgctgacag
     1801 caacatggga aacagaattg agagcatcac tcagcgcatg gcaataatag aaggaaagaa
     1861 taagatggct agcaccttgg ttgtggctga ctctagaatt tctggaatct acatttgcat
     1921 agcttccaat aaagttggga ctgtgggaag aaacataagc ttttatatca cagatgtgcc
     1981 aaatgggttt catgttaact tggaaaaaat gccgacggaa ggagaggacc tgaaactgtc
     2041 ttgcacagtt aacaagttct tatacagaga cgttacttgg attttactgc ggacagttaa
     2101 taacagaaca atgcactaca gtattagcaa gcaaaaaatg gccatcacta aggagcactc
     2161 catcactctt aatcttacca tcatgaatgt ttccctgcaa gattcaggca cctatgcctg
     2221 cagagccagg aatgtataca caggggaaga aatcctccag aagaaagaaa ttacaatcag
     2281 agatcaggaa gcaccatacc tcctgcgaaa cctcagtgat cacacagtgg ccatcagcag
     2341 ttccaccact ttagactgtc atgctaatgg tgtccccgag cctcagatca cttggtttaa
     2401 aaacaaccac aaaatacaac aagagcctgg aattatttta ggaccaggaa gcagcacgct
     2461 gtttattgaa agagtcacag aagaggatga aggtgtctat cactgcaaag ccaccaacca
     2521 gaagggctct gtggaaagtt cagcatacct cactgttcaa ggaacctcgg acaagcctaa
     2581 tctggagctg atcactctaa catgcacctg tgtggctgcg actctcttct ggctcctatt
     2641 aaccctcttt atcggcggtg gctcgggtgg tggctcgaag agcatgtcta gcatggtgtc
     2701 agacactagt tgcacattcc cttcatctga tggcatattc tggaagcatt ggattcaaac
     2761 caaggatggg cagtgtggca gtccattagt atcaactaga gatgggttca ttgttggtat
     2821 acactcagca tcgaatttca ccaacacaaa caattatttc acaagcgtgc cgaaaaactt
     2881 catggaattg ttgacaaatc aggaggcgca gcagtgggtt agtggttggc gattaaatgc
     2941 tgactcagta ttgtgggggg gccataaagt tttcatggtg aaacctgaag agccttttca
     3001 gccagttaag gaagcgactc aactcatgaa tcgtcgtcgc cgtcccggcg gtggctcgga
     3061 aaacctgtat tttcaggggc ccaagaagaa gaggaaggtg gggggaggca gcactagtta
     3121 cccatacgac gtaccagatt acgctggagg ttctggatcc ggcggtggct cgaagcccgc
     3181 cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa
     3241 agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt
     3301 ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct
     3361 gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga
     3421 tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac
     3481 ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg
     3541 ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac
     3601 aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca
     3661 cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga
     3721 tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct
     3781 gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa
     3841 catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg
     3901 cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga
     3961 acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa
     4021 tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt
     4081 ggaccatatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac
     4141 cagaagcgac aaggcccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa
     4201 gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga
     4261 caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa
     4321 gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg
     4381 gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct
     4441 gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat
     4501 caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat
     4561 caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt
     4621 gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt
     4681 ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg
     4741 gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg
     4801 ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac
     4861 cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa
     4921 gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac
     4981 cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa
     5041 gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc
     5101 catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct
     5161 gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg
     5221 cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct
     5281 ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt
     5341 tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa
     5401 gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg
     5461 ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct
     5521 gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag
     5581 caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac
     5641 acggatcgac ctgtctcagc tgggaggcga cagccccaag aagaagagaa aggtggaggc
     5701 cagcggacgg gctgacgcat tggacgattt tgatctggat atgctgggaa gtgacgccct
     5761 cgatgatttt gaccttgaca tgcttggttc ggatgccctt gatgactttg acctcgacat
     5821 gctcggcagt gacgcccttg atgatttcga cctggacatg ctgattaacg gtaccgctag
     5881 cggcagtgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg
     5941 cccagtgagc aagcttatgg cttcaaactt tactcagttc gtgctcgtgg acaatggtgg
     6001 gacaggggat gtgacagtgg ctccttctaa tttcgctaat ggggtggcag agtggatcag
     6061 ctccaactca cggagccagg cctacaaggt gacatgcagc gtcaggcagt ctagtgccca
     6121 gaagagaaag tataccatca aggtggaggt ccccaaagtg gctacccaga cagtgggcgg
     6181 agtcgaactg cctgtcgccg cttggaggtc ctacctgaac atggagctca ctatcccaat
     6241 tttcgctacc aattctgact gtgaactcat cgtgaaggca atgcaggggc tcctcaaaga
     6301 cggtaatcct atcccttccg ccatcgccgc taactcaggt atctacagcg ctggaggagg
     6361 tggaagcgga ggaggaggaa gcggaggagg aggtagcgga cctaagaaaa agaggaaggt
     6421 ggcggccgct ggatcccctt cagggcagat cagcaaccag gccctggctc tggcccctag
     6481 ctccgctcca gtgctggccc agactatggt gccctctagt gctatggtgc ctctggccca
     6541 gccacctgct ccagcccctg tgctgacccc aggaccaccc cagtcactga gcgctccagt
     6601 gcccaagtct acacaggccg gcgaggggac tctgagtgaa gctctgctgc acctgcagtt
     6661 cgacgctgat gaggacctgg gagctctgct ggggaacagc accgatcccg gagtgttcac
     6721 agatctggcc tccgtggaca actctgagtt tcagcagctg ctgaatcagg gcgtgtccat
     6781 gtctcatagt acagccgaac caatgctgat ggagtacccc gaagccatta cccggctggt
     6841 gaccggcagc cagcggcccc ccgaccccgc tccaactccc ctgggaacca gcggcctgcc
     6901 taatgggctg tccggagatg aagacttctc aagcatcgct gatatggact ttagtgccct
     6961 gctgtcacag atttcctcta gtgggcaggg aggaggtgga agcggcttca gcgtggacac
     7021 cagtgccctg ctggacctgt tcagcccctc ggtgaccgtg cccgacatga gcctgcctga
     7081 ccttgacagc agcctggcca gtatccaaga gctcctgtct ccccaggagc cccccaggcc
     7141 tcccgaggca gagaacagca gcccggattc agggaagcag ctggtgcact acacagcgca
     7201 gccgctgttc ctgctggacc ccggctccgt ggacaccggg agcaacgacc tgccggtgct
     7261 gtttgagctg ggagagggct cctacttctc cgaaggggac ggcttcgccg aggaccccac
     7321 catctccctg ctgacaggct cggagcctcc caaagccaag gaccccactg tctcctaaga
     7381 attcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     7441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7561 gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc tggggagcgg
     7621 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     7681 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     7741 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
     7801 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     7861 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
     7921 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7981 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8041 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8101 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8161 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     8221 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     8281 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8341 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     8401 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     8461 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     8521 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     8581 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8641 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8701 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8761 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8821 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8881 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8941 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9001 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9061 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9121 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9181 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9241 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9301 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9361 ggtttattgc tgataaatct ggagccggtg agcgtggaag ccgcggtatc attgcagcac
     9421 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9481 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9541 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9601 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9661 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9721 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9781 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9841 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9901 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9961 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10021 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10081 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10141 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10201 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10261 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10321 ttttacggtt cctggccttt tgctggcctt ttgctcacat gctagaggta cccgttacat
    10381 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
    10441 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
    10501 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
    10561 acggtaaatg gcccgcctgg cattgtgccc agtacatgac cttatgggac tttcctactt
    10621 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
    10681 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
    10741 aattattttg tgcagcgatg ggggcggggg gggggg
//
