LOCUS       sequence-194173-        8534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus expressing puromycin resistance gene and TEV protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Ciabatti E, Gonzalez-Rueda A, Mariotti L, Morgese F, Tripodi M
  TITLE     Life-Long Genetic and Functional Access to Neural Circuits Using 
            Self-Inactivating Rabies Virus.
  JOURNAL   Cell. 2017 Jul 1. pii: S0092-8674(17)30697-9. doi: 
            10.1016/j.cell.2017.06.014.
  PUBMED    28689641
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Jul 1. pii: S0092-8674(17)30697-9. doi: 10.1016/j.cell.2017.06.014."
COMMENT     Sequence Label: pLenti-CMV-puro-2A-TEVp
FEATURES             Location/Qualifiers
     source          1..8534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             599..779
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    826..951
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1444..1677
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1862..1906
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2173..2290
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2392..2695
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2696..2899
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3013..3609
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3013..3032)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3509..3529
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3619..3684
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             3688..4395
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     polyA_signal    4453..4929
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4652..4671)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     LTR             5001..5234
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5306..5427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5343..5362)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5397..5416
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5446..5581
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5601..5620)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5602..5620)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5630..5647)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5630..5646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5639..5661)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5788..6243
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5875..5894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6085..6106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6269..6373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6374..6442,6443..7234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6592..6611)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7405..7993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7894..7913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8147..8164
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8281..8302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8317..8322,8323..8340,8341..8347)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8355..8371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8360..8382
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8379..8395
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8379..8395
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8414..8434
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8416..8434
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccggg
      601 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      661 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      721 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      781 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
      841 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
      901 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
      961 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     1021 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     1081 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     1141 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     1201 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     1261 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     1321 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1381 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1441 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1501 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1561 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1621 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1681 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1741 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1801 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1861 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1921 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1981 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2041 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2101 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     2161 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2221 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2281 tcaaaatttt atcgatcacg agactagcct cgactctaga acgcgtggag ctagttatta
     2341 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     2401 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     2461 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     2521 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     2581 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     2641 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     2701 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     2761 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     2821 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     2881 ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
     2941 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggat tgaattcatc
     3001 cgctagccca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     3061 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     3121 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     3181 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     3241 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     3301 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     3361 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     3421 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     3481 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     3541 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     3601 cccggtgccg gatccggagt gaaacagact ttgaattttg accttctcaa gttggcggga
     3661 gacgtggagt ccaacccagg gcccatggga gaaagcttgt ttaaggggcc gcgtgattac
     3721 aacccgatat cgagcaccat ttgtcatttg acgaatgaat ctgatgggca cacaacatcg
     3781 ttgtatggta ttggatttgg tcccttcatc attacaaaca agcacttgtt tagaagaaat
     3841 aatggaacac tgttggtcca atcactacat ggtgtattca aggtcaagaa caccacgact
     3901 ttgcaacaac acctcattga tgggagggac atgataatta ttcgcatgcc taaggatttc
     3961 ccaccatttc ctcaaaagct gaaatttaga gagccacaaa gggaagagcg catatgtctt
     4021 gtgacaacca acttccaaac taagagcatg tctagcatgg tgtcagacac tagttgcaca
     4081 ttcccttcat ctgatggcat attctggaag cattggattc aaaccaagga tgggcagtgt
     4141 ggcagtccat tagtatcaac tagagatggg ttcattgttg gtatacactc agcatcgaat
     4201 ttcaccaaca caaacaatta tttcacaagc gtgccgaaaa acttcatgga attgttgaca
     4261 aatcaggagg cgcagcagtg ggttagtggt tggcgattaa atgctgactc agtattgtgg
     4321 gggggccata aagttttcat ggtgaaacct gaagagcctt ttcagccagt taaggaagcg
     4381 actcaactca tgaatcgtcg tcgccgtcgc tgataaaagc ttgcctcgag cagcgctgct
     4441 cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     4501 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     4561 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     4621 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     4681 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     4741 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     4801 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     4861 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     4921 cctgtccttg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4981 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5041 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5101 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5161 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5221 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     5281 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     5341 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5401 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     5461 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5521 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5581 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     5641 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     5701 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5761 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     5821 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5881 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5941 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6001 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6061 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     6121 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     6181 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     6241 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6301 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6361 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6421 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6481 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6541 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6601 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6661 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6721 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6781 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6841 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6901 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6961 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7021 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7081 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7141 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7201 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7261 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7321 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7381 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7441 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7501 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7561 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7621 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7681 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7741 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7801 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7861 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7921 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7981 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8041 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8101 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8161 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     8221 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     8281 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     8341 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     8401 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagcttg
     8461 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
     8521 attaccgcca tgtt
//
