LOCUS       sequence-194181-       17268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17268)
  AUTHORS   Ciabatti E, Gonzalez-Rueda A, Mariotti L, Morgese F, Tripodi M
  TITLE     Life-Long Genetic and Functional Access to Neural Circuits Using 
            Self-Inactivating Rabies Virus.
  JOURNAL   Cell. 2017 Jul 1. pii: S0092-8674(17)30697-9. doi: 
            10.1016/j.cell.2017.06.014.
  PUBMED    28689641
REFERENCE   2  (bases 1 to 17268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Jul 1. pii: S0092-8674(17)30697-9. doi: 10.1016/j.cell.2017.06.014."
COMMENT     Sequence Label: pSAD-F3-NPEST-iCRE-2A-mCherryPEST
FEATURES             Location/Qualifiers
     source          1..17268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..45
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1090..1146
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1153..1857
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1295..1313)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1563..1582
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3734..3745
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8843..8860)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8849..9073
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9119..9547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9206..9225)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9416..9437
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9556..9576)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9561..9890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9741..9876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9803..9822
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9938..9985
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10004..10378
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10508..10629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10545..10564)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10599..10618
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10678..10694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10678..10694)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10691..10713)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10702..10718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10726..10732,10733..10750,10751..10756))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10771..10792
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10909..10926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11080..11668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11160..11179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11839..12630,12631..12699))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12462..12481
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12700..12804)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12879..12898)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        13070..13449
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13450..13653
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13604..13624
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     13698..13717
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13698..13716
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             15212..15232
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggccgcggta ccgccaccat ggtgcccaag aagaagagga aagtctccaa cctgctgact
       61 gtgcaccaaa acctgcctgc cctccctgtg gatgccacct ctgatgaagt caggaagaac
      121 ctgatggaca tgttcaggga caggcaggcc ttctctgaac acacctggaa gatgctcctg
      181 tctgtgtgca gatcctgggc tgcctggtgc aagctgaaca acaggaaatg gttccctgct
      241 gaacctgagg atgtgaggga ctacctcctg tacctgcaag ccagaggcct ggctgtgaag
      301 accatccaac agcacctggg ccagctcaac atgctgcaca ggagatctgg cctgcctcgc
      361 ccttctgact ccaatgctgt gtccctggtg atgaggagaa tcagaaagga gaatgtggat
      421 gctggggaga gagccaagca ggccctggcc tttgaacgca ctgactttga ccaagtcaga
      481 tccctgatgg agaactctga cagatgccag gacatcagga acctggcctt cctgggcatt
      541 gcctacaaca ccctgctgcg cattgccgaa attgccagaa tcagagtgaa ggacatctcc
      601 cgcaccgatg gtgggagaat gctgatccac attggcagga ccaagaccct ggtgtccaca
      661 gctggtgtgg agaaggccct gtccctgggg gttaccaagc tggtggagag atggatctct
      721 gtgtctggtg tggctgatga ccccaacaac tacctgttct gccgggtcag aaagaatggt
      781 gtggctgccc cttctgccac ctcccaactg tccacccggg ccctggaagg gatctttgag
      841 gccacccacc gcctgatcta tggtgccaag gatgactctg ggcagagata cctggcctgg
      901 tctggccact ctgccagagt gggtgctgcc agggacatgg ccagggctgg tgtgtccatc
      961 cctgaaatca tgcaggctgg tggctggacc aatgtgaaca ttgtgatgaa ctacatcaga
     1021 aacctggact ctgagactgg ggccatggtg aggctgctcg aggatgggga cggcagtgga
     1081 ggatccggag ccacgaactt ctctctgtta aagcaagcag gagacgtgga agaaaacccc
     1141 ggtcctaccg gtgtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1201 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1261 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     1321 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     1381 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1441 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     1501 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     1561 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     1621 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     1681 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     1741 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     1801 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga
     1861 tatctcagcc atggcttccc gccggaggtg gaggagcagg atgatggcac gctgcccatg
     1921 tcttgtgccc aggagagcgg gatggaccgt caccctgcag cctgtgcttc tgctaggatc
     1981 aatgtgtgac tcgagggcgc gcctacccgc ggtagctttt cagtcgagaa aaaaacatta
     2041 gatcagaaga acaactggca acacttctca acctgagact tacttcaaga tgctcgatcc
     2101 tggagaggtc tatgatgacc ctattgaccc aatcgagtta gaggctgaac ccagaggaac
     2161 ccccattgtc cccaacatct tgaggaactc tgactacaat ctcaactctc ctttgataga
     2221 agatcctgct agactaatgt tagaatggtt aaaaacaggg aatagacctt atcggatgac
     2281 tctaacagac aattgctcca ggtctttcag agttttgaaa gattatttca agaaggtaga
     2341 tttgggttct ctcaaggtgg gcggaatggc tgcacagtca atgatttctc tctggttata
     2401 tggtgcccac tctgaatcca acaggagccg gagatgtata acagacttgg cccatttcta
     2461 ttccaagtcg tcccccatag agaagctgtt gaatctcacg ctaggaaata gagggctgag
     2521 aatcccccca gagggagtgt taagttgcct tgagagggtt gattatgata atgcatttgg
     2581 aaggtatctt gccaacacgt attcctctta cttgttcttc catgtaatca ccttatacat
     2641 gaacgcccta gactgggatg aagaaaagac catcctagca ttatggaaag atttaacctc
     2701 agtggacatc gggaaggact tggtaaagtt caaagaccaa atatggggac tgctgatcgt
     2761 gacaaaggac tttgtttact cccaaagttc caattgtctt tttgacagaa actacacact
     2821 tatgctaaaa gatcttttct tgtctcgctt caactcctta atggtcttgc tctctccccc
     2881 agagccccga tactcagatg acttgatatc tcaactatgc cagctgtaca ttgctgggga
     2941 tcaagtcttg tctatgtgtg gaaactccgg ctatgaagtc atcaaaatat tggagccata
     3001 tgtcgtgaat agtttagtcc agagagcaga aaagtttagg cctctcattc attccttggg
     3061 agactttcct gtatttataa aagacaaggt aagtcaactt gaagagacgt tcggtccctg
     3121 tgcaagaagg ttctttaggg ctctggatca attcgacaac atacatgact tggtttttgt
     3181 gtttggctgt tacaggcatt gggggcaccc atatatagat tatcgaaagg gtctgtcaaa
     3241 actatatgat caggttcacc ttaaaaaaat gatagataag tcctaccagg agtgcttagc
     3301 aagcgaccta gccaggagga tccttagatg gggttttgat aagtactcca agtggtatct
     3361 ggattcaaga ttcctagccc gagaccaccc cttgactcct tatatcaaaa cccaaacatg
     3421 gccacccaaa catattgtag acttggtggg ggatacatgg cacaagctcc cgatcacgca
     3481 gatctttgag attcctgaat caatggatcc gtcagaaata ttggatgaca aatcacattc
     3541 tttcaccaga acgagactag cttcttggct gtcagaaaac cgaggggggc ctgttcctag
     3601 cgaaaaagtt attatcacgg ccctgtctaa gccgcctgtc aatccccgag agtttctgag
     3661 gtctatagac ctcggaggat tgccagatga agacttgata attggcctca agccaaagga
     3721 acgggaattg aagattgaag gtcgattctt tgctctaatg tcatggaatc taagattgta
     3781 ttttgtcatc actgaaaaac tcttggccaa ctacatcttg ccactttttg acgcgctgac
     3841 tatgacagac aacctgaaca aggtgtttaa aaagctgatc gacagggtca ccgggcaagg
     3901 gcttttggac tattcaaggg tcacatatgc atttcacctg gactatgaaa agtggaacaa
     3961 ccatcaaaga ttagagtcaa cagaggatgt attttctgtc ctagatcaag tgtttggatt
     4021 gaagagagtg ttttctagaa cacacgagtt ttttcaaaag gcctggatct attattcaga
     4081 cagatcagac ctcatcgggt tacgggagga tcaaatatac tgcttagatg cgtccaacgg
     4141 cccaacctgt tggaatggcc aggatggcgg gctagaaggc ttacggcaga agggctggag
     4201 tctagtcagc ttattgatga tagatagaga atctcaaatc aggaacacaa gaaccaaaat
     4261 actagctcaa ggagacaacc aggttttatg tccgacatac atgttgtcgc cagggctatc
     4321 tcaagagggg ctcctctatg aattggagag aatatcaagg aatgcacttt cgatatacag
     4381 agccgtcgag gaaggggcat ctaagctagg gctgatcatc aagaaagaag agaccatgtg
     4441 tagttatgac ttcctcatct atggaaaaac ccctttgttt agaggtaaca tattggtgcc
     4501 tgagtccaaa agatgggcca gagtctcttg cgtctctaat gaccaaatag tcaacctcgc
     4561 caatataatg tcgacagtgt ccaccaatgc gctaacagtg gcacaacact ctcaatcttt
     4621 gatcaaaccg atgagggatt ttctgctcat gtcagtacag gcagtctttc actacctgct
     4681 atttagccca atcttaaagg gaagagttta caagattctg agcgctgaag gggagagctt
     4741 tctcctagcc atgtcaagga taatctatct agatccttct ttgggaggga tatctggaat
     4801 gtccctcgga agattccata tacgacagtt ctcagaccct gtctctgaag ggttatcctt
     4861 ctggagagag atctggttaa gctcccaaga gtcctggatt cacgcgttgt gtcaagaggc
     4921 tggaaaccca gatcttggag agagaacact cgagagcttc actcgccttc tagaagatcc
     4981 gaccacctta aatatcagag gaggggccag tcctaccatt ctactcaagg atgcaatcag
     5041 aaaggcttta tatgacgagg tggacaaggt ggaaaattca gagtttcgag aggcaatcct
     5101 gttgtccaag acccatagag ataattttat actcttctta atatctgttg agcctctgtt
     5161 tcctcgattt ctcagtgagc tattcagttc gtcttttttg ggaatccccg agtcaatcat
     5221 tggattgata caaaactccc gaacgataag aaggcagttt agaaagagtc tctcaaaaac
     5281 tttagaagaa tccttctaca actcagagat ccacgggatt agtcggatga cccagacacc
     5341 tcagagggtt gggggggtgt ggccttgctc ttcagagagg gcagatctac ttagggagat
     5401 ctcttgggga agaaaagtgg taggcacgac agttcctcac ccttctgaga tgttgggatt
     5461 acttcccaag tcctctattt cttgcacttg tggagcaaca ggaggaggca atcctagagt
     5521 ttctgtatca gtactcccgt cctttgatca gtcatttttt tcacgaggcc ccctaaaggg
     5581 atacttgggc tcgtccacct ctatgtcgac ccagctattc catgcatggg aaaaagtcac
     5641 taatgttcat gtggtgaaga gagctctatc gttaaaagaa tctataaact ggttcattac
     5701 tagagattcc aacttggctc aagctctaat taggaacatt atgtctctga caggccctga
     5761 tttccctcta gaggaggccc ctgtcttcaa aaggacgggg tcagccttgc ataggttcaa
     5821 gtctgccaga tacagcgaag gagggtattc ttctgtctgc ccgaacctcc tctctcatat
     5881 ttctgttagt acagacacca tgtctgattt gacccaagac gggaagaact acgatttcat
     5941 gttccagcca ttgatgcttt atgcacagac atggacatca gagctggtac agagagacac
     6001 aaggctaaga gactctacgt ttcattggca cctccgatgc aacaggtgtg tgagacccat
     6061 tgacgacgtg accctggaga cctctcagat cttcgagttt ccggatgtgt cgaaaagaat
     6121 atccagaatg gtttctgggg ctgtgcctca cttccagagg cttcccgata tccgtctgag
     6181 accaggagat tttgaatctc taagcggtag agaaaagtct caccatatcg gatcagctca
     6241 ggggctctta tactcaatct tagtggcaat tcacgactca ggatacaatg atggaaccat
     6301 cttccctgtc aacatatacg gcaaggtttc ccctagagac tatttgagag ggctcgcaag
     6361 gggagtattg ataggatcct cgatttgctt cttgacaaga atgacaaata tcaatattaa
     6421 tagacctctt gaattggtct caggggtaat ctcatatatt ctcctgaggc tagataacca
     6481 tccctccttg tacataatgc tcagagaacc gtctcttaga ggagagatat tttctatccc
     6541 tcagaaaatc cccgccgctt atccaaccac tatgaaagaa ggcaacagat caatcttgtg
     6601 ttatctccaa catgtgctac gctatgagcg agagataatc acggcgtctc cagagaatga
     6661 ctggctatgg atcttttcag actttagaag tgccaaaatg acgtacctat ccctcattac
     6721 ttaccagtct catcttctac tccagagggt tgagagaaac ctatctaaga gtatgagaga
     6781 taacctgcga caattgagtt ctttgatgag gcaggtgctg ggcgggcacg gagaagatac
     6841 cttagagtca gacgacaaca ttcaacgact gctaaaagac tctttacgaa ggacaagatg
     6901 ggtggatcaa gaggtgcgcc atgcagctag aaccatgact ggagattaca gccccaacaa
     6961 gaaggtgtcc cgtaaggtag gatgttcaga atgggtctgc tctgctcaac aggttgcagt
     7021 ctctacctca gcaaacccgg cccctgtctc ggagcttgac ataagggccc tctctaagag
     7081 gttccagaac cctttgatct cgggcttgag agtggttcag tgggcaaccg gtgctcatta
     7141 taagcttaag cctattctag atgatctcaa tgttttccca tctctctgcc ttgtagttgg
     7201 ggacgggtca ggggggatat caagggcagt cctcaacatg tttccagatg ccaagcttgt
     7261 gttcaacagt cttttagagg tgaatgacct gatggcttcc ggaacacatc cactgcctcc
     7321 ttcagcaatc atgaggggag gaaatgatat cgtctccaga gtgatagatc ttgactcaat
     7381 ctgggaaaaa ccgtccgact tgagaaactt ggcaacctgg aaatacttcc agtcagtcca
     7441 aaagcaggtc aacatgtcct atgacctcat tatttgcgat gcagaagtta ctgacattgc
     7501 atctatcaac cggatcaccc tgttaatgtc cgattttgca ttgtctatag atggaccact
     7561 ctatttggtc ttcaaaactt atgggactat gctagtaaat ccaaactaca aggctattca
     7621 acacctgtca agagcgttcc cctcggtcac agggtttatc acccaagtaa cttcgtcttt
     7681 ttcatctgag ctctacctcc gattctccaa acgagggaag tttttcagag atgctgagta
     7741 cttgacctct tccacccttc gagaaatgag ccttgtgtta ttcaattgta gcagccccaa
     7801 gagtgagatg cagagagctc gttccttgaa ctatcaggat cttgtgagag gatttcctga
     7861 agaaatcata tcaaatcctt acaatgagat gatcataact ctgattgaca gtgatgtaga
     7921 atcttttcta gtccacaaga tggttgatga tcttgagtta cagaggggaa ctctgtctaa
     7981 agtggctatc attatagcca tcatgatagt tttctccaac agagtcttca acgtttccaa
     8041 acccctaact gacccctcgt tctatccacc gtctgatccc aaaatcctga ggcacttcaa
     8101 catatgttgc agtactatga tgtatctatc tactgcttta ggtgacgtcc ctagcttcgc
     8161 aagacttcac gacctgtata acagacctat aacttattac ttcagaaagc aagtcattcg
     8221 agggaacgtt tatctatctt ggagttggtc caacgacacc tcagtgttca aaagggtagc
     8281 ctgtaattct agcctgagtc tgtcatctca ctggatcagg ttgatttaca agatagtgaa
     8341 gactaccaga ctcgttggca gcatcaagga tctatccaga gaagtggaaa gacaccttca
     8401 taggtacaac aggtggatca ccctagagga tatcagatct agatcatccc tactagacta
     8461 cagttgcctg tgaaccggat actcctggaa gcctgcccat gctaagactc ttgtgtgatg
     8521 tatcttgaaa aaaacaagat cctaaatctg aacctttggt tgtttgattg tttttctcat
     8581 ttttgttgtt tatttgttaa gcgtgggtcg gcatggcatc tccacctcct cgcggtccga
     8641 cctgggcatc cgaaggagga cgcacgtcca ctcggatggc taagggagaa gggcgaattc
     8701 cagcacactg gcggccgtta ctagtggatc cgagctcggt accgagctcg gatccactag
     8761 tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg
     8821 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     8881 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     8941 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     9001 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     9061 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     9121 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     9181 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     9241 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     9301 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     9361 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     9421 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     9481 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     9541 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     9601 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     9661 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     9721 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     9781 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     9841 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     9901 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     9961 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
    10021 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
    10081 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
    10141 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
    10201 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
    10261 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
    10321 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
    10381 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    10441 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    10501 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10561 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10621 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    10681 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    10741 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    10801 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    10861 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    10921 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    10981 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    11041 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    11101 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    11161 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    11221 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    11281 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    11341 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    11401 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    11461 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    11521 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    11581 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    11641 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    11701 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    11761 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    11821 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    11881 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    11941 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    12001 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    12061 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    12121 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    12181 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    12241 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    12301 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    12361 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    12421 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    12481 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    12541 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    12601 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    12661 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    12721 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    12781 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    12841 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    12901 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    12961 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
    13021 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
    13081 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
    13141 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
    13201 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
    13261 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
    13321 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    13381 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    13441 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
    13501 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
    13561 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
    13621 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
    13681 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagattaag
    13741 cgtctgatga gtccgtgagg acgaaacccg gagtcccggg tcacgcttaa caaccagatc
    13801 aaagaaaaaa cagacattgt caattgcaaa gcaaaaatgt aacaccccta caatggatgc
    13861 cgacaagatt gtattcaaag tcaataatca ggtggtctct ttgaagcctg agattatcgt
    13921 ggatcaatat gagtacaagt accctgccat caaagatttg aaaaagccct gtataaccct
    13981 aggaaaggct cccgatttaa ataaagcata caagtcagtt ttgtcaggca tgagcgccgc
    14041 caaacttaat cctgacgatg tatgttccta tttggcagcg gcaatgcagt tttttgaggg
    14101 gacatgtccg gaagactgga ccagctatgg aattgtgatt gcacgaaaag gagataagat
    14161 caccccaggt tctctggtgg agataaaacg tactgatgta gaagggaatt gggctctgac
    14221 aggaggcatg gaactgacaa gagaccccac tgtccctgag catgcgtcct tagtcggtct
    14281 tctcttgagt ctgtataggt tgagcaaaat atccgggcaa aacactggta actataagac
    14341 aaacattgca gacaggatag agcagatttt tgagacagcc ccttttgtta aaatcgtgga
    14401 acaccatact ctaatgacaa ctcacaaaat gtgtgctaat tggagtacta taccaaactt
    14461 cagatttttg gccggaacct atgacatgtt tttctcccgg attgagcatc tatattcagc
    14521 aatcagagtg ggcacagttg tcactgctta tgaagactgt tcaggactgg tatcatttac
    14581 tgggttcata aaacaaatca atctcaccgc tagagaggca atactatatt tcttccacaa
    14641 gaactttgag gaagagataa gaagaatgtt tgagccaggg caggagacag ctgttcctca
    14701 ctcttatttc atccacttcc gttcactagg cttgagtggg aaatctcctt attcatcaaa
    14761 tgctgttggt cacgtgttca atctcattca ctttgtagga tgctatatgg gtcaagtcag
    14821 atccctaaat gcaacggtta ttgctgcatg tgctcctcat gaaatgtctg ttctaggggg
    14881 ctatctggga gaggaattct tcgggaaagg gacatttgaa agaagattct tcagagatga
    14941 gaaagaactt caagaatacg aggcggctga actgacaaag actgacgtag cactggcaga
    15001 tgatggaact gtcaactctg acgacgagga ctacttttca ggtgaaacca gaagtccgga
    15061 ggctgtttat actcgaatca tgatgaatgg aggtcgacta aagagatctc acatacggag
    15121 atatgtctca gtcagttcca atcatcaagc ccgtccaaac tcattcgccg agtttctaaa
    15181 caagacatat tcgagtgact caggttccgg agagaacctc tacttccaat cgggatccgg
    15241 tagccatggc ttcccgccgg aggtggagga gcaggatgat ggcacgctgc ccatgtcttg
    15301 tgcccaggag agcgggatgg accgtcaccc tgcagcctgt gcttctgcta ggatcaatgt
    15361 gtaagaagtt gaataacaaa atgccggaaa tctacggatt gtgtatatcc atcatgaaaa
    15421 aaactaacac ccctcctttc gaaccatccc aaacatgagc aagatctttg tcaatcctag
    15481 tgctattaga gccggtctgg ccgatcttga gatggctgaa gaaactgttg atctgatcaa
    15541 tagaaatatc gaagacaatc aggctcatct ccaaggggaa cccatagagg tggacaatct
    15601 ccctgaggat atggggcgac ttcacctgga tgatggaaaa tcgcccaacc atggtgagat
    15661 agccaaggtg ggagaaggca agtatcgaga ggactttcag atggatgaag gagaggatcc
    15721 tagcttcctg ttccagtcat acctggaaaa tgttggagtc caaatagtca gacaaatgag
    15781 gtcaggagag agatttctca agatatggtc acagaccgta gaagagatta tatcctatgt
    15841 cgcggtcaac tttcccaacc ctccaggaaa gtcttcagag gataaatcaa cccagactac
    15901 tggccgagag ctcaagaagg agacaacacc cactccttct cagagagaaa gccaatcatc
    15961 gaaagccagg atggcggctc aaattgcttc tggccctcca gcccttgaat ggtcggctac
    16021 caatgaagag gatgatctat cagtggaggc tgagatcgct caccagattg cagaaagttt
    16081 ctccaaaaaa tataagtttc cctctcgatc ctcagggata ctcttgtata attttgagca
    16141 attgaaaatg aaccttgatg atatagttaa agaggcaaaa aatgtaccag gtgtgacccg
    16201 tttagcccat gacgggtcca aactccccct aagatgtgta ctgggatggg tcgctttggc
    16261 caactctaag aaattccagt tgttagtcga atccgacaag ctgagtaaaa tcatgcaaga
    16321 tgacttgaat cgctatacat cttgctaacc gaacctctcc cctcagtccc tctagacaat
    16381 aaaatccgag atgtcccaaa gtcaacatga aaaaaacagg caacaccact gataaatcga
    16441 tgaacctcct acgtaagata gtgaaaaacc gcagggacga ggacactcaa aaatcctctc
    16501 ccgcgtcagc ccctctggat gacgatgact tgtggcttcc accccctgaa tacgtcccgc
    16561 tgaaagaact tacaggcaag aagaacatga ggaacttttg tatcaacgga agggttaaag
    16621 tgtgtagccc gaatggttac tcgttcagga tcctgcggca cattctgaaa tcattcgacg
    16681 agatatattc tgggaatcat aggatgatcg ggttagtcaa agtggttatt ggactggctt
    16741 tgtcaggatc tccagtccct gagggcctga actgggtata caaattgagg agaaccttta
    16801 tcttccagtg ggctgattcc aggggccctc ttgaagggga ggagttggaa tactctcagg
    16861 agatcacttg ggatgatgat actgagttcg tcggattgca aataagagtg attgcaaaac
    16921 agtgtcatat ccagggcaga gtctggtgta tcaacatgaa cccgagagca tgtcaactat
    16981 ggtctgacat gtctcttcag acacaaaggt ccgaagagga caaagattcc tctctgcttc
    17041 tagaataacg tacgtcagat tatatcccgc aaatttatca cttgtttacc tctggaggag
    17101 agaacatatg ggctcaactc caacccttgg gagcaatata acaaaaaaca tgttatggtg
    17161 ccattaaacc gctgcatttc atcaaagtca agttgattac ctttacattt tgatcctctt
    17221 ggatgtgaaa aaaactatta acatccctca aaggacctgc aggtacgc
//
