LOCUS       sequence-194226-        3637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea Ringo/SPY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA
  TITLE     A premeiotic function for boule in the planarian Schmidtea 
            mediterranea.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 
            10.1073/pnas.1521341113. Epub 2016 Jun 2.
  PUBMED    27330085
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521341113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: 
            "E3509-18"
COMMENT     Sequence Label: Ringo/SPY
FEATURES             Location/Qualifiers
     source          1..3637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(516..534)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(517..534)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(538..556)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      570..617
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(629..646)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(800..1388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(880..899)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1559..2350,2351..2419))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2182..2201
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2437..3231)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2548..2567)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3158..3177
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagaattaac cctcactaaa gggagacctt gctcacgatg
      121 tcgaagaaga caatgaggat ttgaaatatg aaatatttcc atgggttctt ggtccatatt
      181 ggcggaataa catatcagtg tttcttcaga aaaaagatga tatatgggca cgaatgaatt
      241 atagggccat tgtttgtaaa aaatgttgtg aagaactaat gtctatttac cctaaccact
      301 ctatatggca acgagaaaga ccttatcatc ttggtggcgc aattagaaat tacaaaaagg
      361 atatcaaatt atctgttcca agaggtccca atttgtctcc taaaacttgt tttaaatgtc
      421 acccagaatt gagtacaagt cttcacgatt ctgctttcag tagtctttgg gaatgtgaaa
      481 gctttaatga cagcgacgag tcagataagg tcacttctat agtgtcacct aaatctccct
      541 atagtgagtc gtattaggat ccggcgcgcc tagcataacc ccttggggcc tctaaacggg
      601 tcttgagggg ttttttgacg cgtgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      661 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      721 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      781 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      841 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      901 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      961 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1021 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1081 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1141 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1201 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1261 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1321 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1381 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1441 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1501 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1561 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1621 ttgcctggct ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1681 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1741 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1801 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1861 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1921 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1981 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2041 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2101 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2161 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2221 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2281 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2341 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2401 ggaaatgttg aatactcata ctcttccttt ttcaattcag aagaactcgt caagaaggcg
     2461 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     2521 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     2581 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     2641 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     2701 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     2761 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     2821 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     2881 agccatgatg gatactttct cggcaggagc aaggtgggat gacaggagat cctgccccgg
     2941 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     3001 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     3061 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     3121 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     3181 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     3241 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatca ttggcggcaa
     3301 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     3361 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
     3421 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
     3481 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt
     3541 cctttagcag cccttgcgcc ctgaattttg ttaaaattcg cgttaaattt ttgttaaatc
     3601 agctcatttt ttaaccaata ggccgaaatc gacgcgt
//
