LOCUS       sequence-194242-        4032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea TPX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA
  TITLE     A premeiotic function for boule in the planarian Schmidtea 
            mediterranea.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 
            10.1073/pnas.1521341113. Epub 2016 Jun 2.
  PUBMED    27330085
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521341113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: 
            "E3509-18"
COMMENT     Sequence Label: TPX1
FEATURES             Location/Qualifiers
     source          1..4032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(911..929)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(912..929)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(933..951)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      965..1012
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1024..1041)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1195..1783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1275..1294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1954..2745,2746..2814))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2577..2596
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2832..3626)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2943..2962)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3553..3572
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagaattaac cctcactaaa gggagacctt tggagttgct
      121 gtcgagattg atcggctatc acaaattgca attggttaat ttctattcct atatcgttgg
      181 gtttctcaac atccgcacgg ccgacgtgac aaacttgctg gtatatgccg cccaggcgag
      241 ccatgaattc gtgtcacctg atgacattca gccggttgtc aagatgattg ccgatcaatt
      301 cgcgattgac gggatatccg agcagggaat cgcgttggga ttgaactcga tccgtgagat
      361 ctgcgtacgg tgtccccatg caatgccaga tgagctgctg gcaccgttgc tagagttcat
      421 taagcatcga gacaaaaatg tcatctctgc tgccagatct ctgctgagac tgtatcgaga
      481 gatcaatccc gctatgttac cgaaatcatt gcaaggccga ccgaccgaag ctacagttga
      541 tgttggtgtt cgaggagatg cggctcggtt gccaacggct ttcggtgtgt tgaattcggt
      601 ggcgttcatt cccggggcag aaatcattga gaaggaacaa gcagaggaag gggcccagga
      661 ggacgaggaa tatgagtcct gcagtgacat cagcgacgaa gaggaatacg aagattgggt
      721 tgatattcag cagtcggacg aggaagaaat gcccgacccg aaggagttgc gagacccgga
      781 agaaagacgg aaactcgccg aagagatgtc gtatagccac actttcactg acgaggagca
      841 tttggctatc aaaaagcatc tactaaagaa aaaagagata ggagcaagca aaaaccgaaa
      901 gaaggtcact tctatagtgt cacctaaatc tccctatagt gagtcgtatt aggatccggc
      961 gcgcctagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgacgcgtgc
     1021 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1081 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1141 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1201 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1261 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1381 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1501 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1561 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1621 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1681 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1741 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1801 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1861 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1921 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1981 tatctcagcg atctgtctat ttcgttcatc catagttgcc tggctccccg tcgtgtagat
     2041 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2101 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2161 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2221 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2281 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2341 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2401 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2461 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2521 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2581 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2641 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2701 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2761 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2821 cctttttcaa ttcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     2881 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     2941 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     3001 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     3061 ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
     3121 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     3181 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     3241 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     3301 ggagcaaggt gggatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     3361 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     3421 cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg
     3481 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     3541 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     3601 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
     3661 cttgatcccc tgcgccatca gatcattggc ggcaagaaag ccatccagtt tactttgcag
     3721 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     3781 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     3841 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     3901 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgaa
     3961 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     4021 aaatcgacgc gt
//
