LOCUS       sequence-194245-        3691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea TRF2-3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA
  TITLE     A premeiotic function for boule in the planarian Schmidtea 
            mediterranea.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 
            10.1073/pnas.1521341113. Epub 2016 Jun 2.
  PUBMED    27330085
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521341113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: 
            "E3509-18"
COMMENT     Sequence Label: TRF2-3
FEATURES             Location/Qualifiers
     source          1..3691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     84..101
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(570..588)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(592..610)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      624..671
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1078..1872
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1132..1151)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1742..1761
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(1890..1958,1959..2750)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2108..2127)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2921..3509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3410..3429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3663..3680
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagatttagg tgacactata gaagtgacct ttatcgcctg
      121 ctttttcgac ttgaactgcg gattggatct aagtcatata gccaaatcag ctctaaatac
      181 aaaatatgat gcaaaatgtt ttccggcggt aataatgaga ataagaaaac cgaaaactac
      241 agcgaatata tttaaaaatg gaaaaataat ttgcgctggt gctaaaacag aagaagatgc
      301 taaaaaagca acgagaaaaa tctctaggat tattcaaaaa ttaggctaca aaattagata
      361 taataacttt aaaattctta acatggttgc gtcgtgcgac gtaagatttc aaattggttt
      421 agatcctttg aatttgctcc atagttcatt tacaacttat gatccagaac agtttcccgg
      481 tctcttttac aaaatggcag atccattaat tacaatttta atttttaaat ctgggaaaat
      541 tattttgacc ggaggaaaag aaaggtctcc ctttagtgag ggttaattct ccctatagtg
      601 agtcgtatta ggatccggcg cgcctagcat aaccccttgg ggcctctaaa cgggtcttga
      661 ggggtttttt gacgcgtcga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
      721 atttaacgcg aattttaaca aaattcaggg cgcaagggct gctaaaggaa gcggaacacg
      781 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
      841 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
      901 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
      961 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaatg
     1021 atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg
     1081 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     1141 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     1201 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     1261 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     1321 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     1381 ctcctgtcat cccaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     1441 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     1501 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     1561 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     1621 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     1681 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     1741 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     1801 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     1861 gagttcttct gaattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1921 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1981 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2041 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2101 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2161 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2221 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2281 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2341 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2401 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2461 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2521 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2581 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2641 gtaagccctc ccgtatcgta gttatctaca cgacggggag ccaggcaact atggatgaac
     2701 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2761 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2821 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2881 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2941 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3001 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3061 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3121 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3181 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3241 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3301 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3361 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3421 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3481 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3541 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3601 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3661 gcagcgagtc agtgagcgag gaagcacgcg t
//
