LOCUS       sequence-194246-        3912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea TSSK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA
  TITLE     A premeiotic function for boule in the planarian Schmidtea 
            mediterranea.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 
            10.1073/pnas.1521341113. Epub 2016 Jun 2.
  PUBMED    27330085
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521341113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: 
            "E3509-18"
COMMENT     Sequence Label: TSSK
FEATURES             Location/Qualifiers
     source          1..3912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(791..809)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(792..809)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(813..831)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      845..892
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(904..921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1075..1663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1155..1174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1834..2625,2626..2694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2457..2476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2712..3506)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2823..2842)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3433..3452
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagaattaac cctcactaaa gggagacctt ttgctggaaa
      121 tcgagaacag tcgccgtttt ctcggaacac tcctgaacga gcaacaattc agcatgttcc
      181 acaaaacaaa cgagaacgaa tccaattgga aacaaaagtg ctgagcggcg taggaggtcc
      241 cagtggtcca agtaacttgc caatagcttt gggtggttca attagtcacc cacagccccc
      301 taaaaaagag aagatcaacc gtcactcaaa aaaaccttcc gatgataacc tgaatgatca
      361 aatcaacatt tccaacaaca aaccgaaaaa gaaatcagtc aattctttct ttaagtcttt
      421 gacaacaaaa atatcaaaaa agcaggcatc acaagtagat gacgttgata tcagaccgac
      481 tttatggatg aggcacgcaa ctactcatga aaagttatcc aatccatctc ctagtattaa
      541 tcgatccccg tcaaatcagg attctgcgtc aatgggtcaa ggtacgatgc agaggtcaaa
      601 tgttaatcaa aatcggtaca ttgacacgaa agaaaagtgg tcaaatttta ctgctgaagc
      661 ttcaccatct ccatctccat attttcctga caggtctgga agatatggcc aatctaataa
      721 agccactaat tttgacatgg aaaatctcat aacaagcagc tgtgcaacga gtctgacgag
      781 taaggtcact tctatagtgt cacctaaatc tccctatagt gagtcgtatt aggatccggc
      841 gcgcctagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgacgcgtgc
      901 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      961 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1021 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1081 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1141 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1201 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1261 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1321 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1381 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1441 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1501 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1561 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1621 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1681 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1741 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1801 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1861 tatctcagcg atctgtctat ttcgttcatc catagttgcc tggctccccg tcgtgtagat
     1921 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1981 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2041 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2101 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2161 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2221 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2281 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2341 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2401 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2461 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2521 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2581 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2641 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2701 cctttttcaa ttcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     2761 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     2821 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     2881 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     2941 ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
     3001 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     3061 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     3121 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     3181 ggagcaaggt gggatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     3241 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     3301 cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg
     3361 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     3421 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     3481 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
     3541 cttgatcccc tgcgccatca gatcattggc ggcaagaaag ccatccagtt tactttgcag
     3601 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     3661 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     3721 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     3781 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgaa
     3841 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3901 aaatcgacgc gt
//
