LOCUS       sequence-194247-        3837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea vasa1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA
  TITLE     A premeiotic function for boule in the planarian Schmidtea 
            mediterranea.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 
            10.1073/pnas.1521341113. Epub 2016 Jun 2.
  PUBMED    27330085
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521341113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: 
            "E3509-18"
COMMENT     Sequence Label: vasa1
FEATURES             Location/Qualifiers
     source          1..3837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(716..734)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(717..734)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(738..756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      770..817
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(829..846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1000..1588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1080..1099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1759..2550,2551..2619))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2382..2401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2637..3431)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2748..2767)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3358..3377
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagaattaac cctcactaaa gggagacctt ttgaccccag
      121 tgcaaaaata tgcaattccg attatcgatt ccaaacgcga cctcatggca tgcgcacaaa
      181 ccggttctgg caaaactgcg gcttttctaa tcccgataat caaatctctc agtgagaatg
      241 ggacggaatc tccagcatcg gcagtcgcgt ttccgaaagc tttaataatg gctccaacta
      301 gggagttgtg tcgtcagata ttcactgctg cgcgtcatct ctgtcgcggt tccaatatca
      361 aatgtgccta tatttacggt ggaattgaaa tgaacaaatc ccgacgtaat atacaagcaa
      421 ccggttgcga tatcctcgtg gcgactccag gccgattgat tcatttcctg gaattagttt
      481 ggctctcttt gagatatttg cagttttttg tgctcgatga ggccgatcga atgctcgatt
      541 ccgatggatt ttatgaaagc gttacgaaaa tctacaacga ggccaatttt agtggagacg
      601 atcgatccaa atttcgatgt ttagtgcaac tttccccaat gaaattcaaa ctttagcgag
      661 aaatctcctg aaaaattatc tatttctagc tgttggagtt gttggcaagg tcacttctat
      721 agtgtcacct aaatctccct atagtgagtc gtattaggat ccggcgcgcc tagcataacc
      781 ccttggggcc tctaaacggg tcttgagggg ttttttgacg cgtgcttcct cgctcactga
      841 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      901 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      961 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1021 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1081 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1141 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1201 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1261 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1321 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1381 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1441 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1501 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1561 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1621 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1681 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1741 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1801 tctatttcgt tcatccatag ttgcctggct ccccgtcgtg tagataacta cgatacggga
     1861 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1921 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1981 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2041 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2101 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2161 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2221 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2281 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2341 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2401 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2461 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2521 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2581 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaattcag
     2641 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     2701 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     2761 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     2821 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     2881 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     2941 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     3001 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     3061 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgggat
     3121 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     3181 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     3241 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     3301 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     3361 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     3421 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc
     3481 catcagatca ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     3541 ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct
     3601 agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc
     3661 cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg
     3721 gctttctacg tgttccgctt cctttagcag cccttgcgcc ctgaattttg ttaaaattcg
     3781 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc gacgcgt
//
