LOCUS       sequence-194256-       12204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and
            U3 LTR sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   McCauley SM, Kim K, Nowosielska A, Dauphin A, Yurkovetskiy L, Diehl 
            WE, Luban J
  TITLE     Intron-containing RNA from the HIV-1 provirus activates type I 
            interferon and inflammatory cytokines.
  JOURNAL   Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2.
  PUBMED    30546110
REFERENCE   2  (bases 1 to 12204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-13"; volume: "9(1):5305. doi"; pages: " 
            10.1038/s41467-018-07753-2"
COMMENT     Sequence Label: pBS NL4-3 envFS eGFP Delta-nef/U3
FEATURES             Location/Qualifiers
     source          1..12204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     LTR             833..1090
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             1246..2748
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2541..5552
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    5237..5354
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8217..8450
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8635..8679
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8973..8999
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(9266..9268,9269..9982)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(9266..9268,9269..9979)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9308..9329)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9569..9588)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9916..9937
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             10127..10384
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10460..11048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10540..10559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11219..12010,12011..12079))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11842..11861
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12080..12184)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatatttt aattaatgga agggctaatt tggtcccaaa
      481 aaagacaaga gatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
      541 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgcttcaagt
      601 tagtaccagt tgaaccagag caagtagaag aggccaatga aggagagaac aacagcttgt
      661 tacaccctat gagccagcat gggatggagg acccggaggg agaagtatta gtgtggaagt
      721 ttgacagcct cctagcattt cgtcacatgg cccgagagct gcatccggag tactacaaag
      781 actgctgaca tcgagctttc tacaagggac tttccgctgg ggactttcca gggaggtgtg
      841 gcctgggcgg gactggggag tggcgagccc tcagatgcta catataagca gctgcttttt
      901 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      961 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgctcaaagt agtgtgtgcc
     1021 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     1081 atctctagca gtggcgcccg aacagggact tgaaagcgaa agtaaagcca gaggagatct
     1141 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     1201 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     1261 tcggtattaa gcgggggaga attagataaa tgggaaaaaa ttcggttaag gccaggggga
     1321 aagaaacaat ataaactaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1381 gttaatcctg gccttttaga gacatcagaa ggctgtagac aaatactggg acagctacaa
     1441 ccatcccttc agacaggatc agaagaactt agatcattat ataatacaat agcagtcctc
     1501 tattgtgtgc atcaaaggat agatgtaaaa gacaccaagg aagccttaga taagatagag
     1561 gaagagcaaa acaaaagtaa gaaaaaggca cagcaagcag cagctgacac aggaaacaac
     1621 agccaggtca gccaaaatta ccctatagtg cagaacctcc aggggcaaat ggtacatcag
     1681 gccatatcac ctagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc
     1741 ccagaagtaa tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaat
     1801 accatgctaa acacagtggg gggacatcaa gcagccatgc aaatgttaaa agagaccatc
     1861 aatgaggaag ctgcagaatg ggatagattg catccagtgc atgcagggcc tattgcacca
     1921 ggccagatga gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa
     1981 caaataggat ggatgacaca taatccacct atcccagtag gagaaatcta taaaagatgg
     2041 ataatcctgg gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata
     2101 agacaaggac caaaggaacc ctttagagac tatgtagacc gattctataa aactctaaga
     2161 gccgagcaag cttcacaaga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat
     2221 gcgaacccag attgtaagac tattttaaaa gcattgggac caggagcgac actagaagaa
     2281 atgatgacag catgtcaggg agtgggggga cccggccata aagcaagagt tttggctgaa
     2341 gcaatgagcc aagtaacaaa tccagctacc ataatgatac agaaaggcaa ttttaggaac
     2401 caaagaaaga ctgttaagtg tttcaattgt ggcaaagaag ggcacatagc caaaaattgc
     2461 agggccccta ggaaaaaggg ctgttggaaa tgtggaaagg aaggacacca aatgaaagat
     2521 tgtactgaga gacaggctaa ttttttaggg aagatctggc cttcccacaa gggaaggcca
     2581 gggaattttc ttcagagcag accagagcca acagccccac cagaagagag cttcaggttt
     2641 ggggaagaga caacaactcc ctctcagaag caggagccga tagacaagga actgtatcct
     2701 ttagcttccc tcagatcact ctttggcagc gacccctcgt cacaataaag ataggggggc
     2761 aattaaagga agctctatta gatacaggag cagatgatac agtattagaa gaaatgaatt
     2821 tgccaggaag atggaaacca aaaatgatag ggggaattgg aggttttatc aaagtaagac
     2881 agtatgatca gatactcata gaaatctgcg gacataaagc tataggtaca gtattagtag
     2941 gacctacacc tgtcaacata attggaagaa atctgttgac tcagattggc tgcactttaa
     3001 attttcccat tagtcctatt gagactgtac cagtaaaatt aaagccagga atggatggcc
     3061 caaaagttaa acaatggcca ttgacagaag aaaaaataaa agcattagta gaaatttgta
     3121 cagaaatgga aaaggaagga aaaatttcaa aaattgggcc tgaaaatcca tacaatactc
     3181 cagtatttgc cataaagaaa aaagacagta ctaaatggag aaaattagta gatttcagag
     3241 aacttaataa gagaactcaa gatttctggg aagttcaatt aggaatacca catcctgcag
     3301 ggttaaaaca gaaaaaatca gtaacagtac tggatgtggg cgatgcatat ttttcagttc
     3361 ccttagataa agacttcagg aagtatactg catttaccat acctagtata aacaatgaga
     3421 caccagggat tagatatcag tacaatgtgc ttccacaggg atggaaagga tcaccagcaa
     3481 tattccagtg tagcatgaca aaaatcttag agccttttag aaaacaaaat ccagacatag
     3541 tcatctatca atacatggat gatttgtatg taggatctga cttagaaata gggcagcata
     3601 gaacaaaaat agaggaactg agacaacatc tgttgaggtg gggatttacc acaccagaca
     3661 aaaaacatca gaaagaacct ccattccttt ggatgggtta tgaactccat cctgataaat
     3721 ggacagtaca gcctatagtg ctgccagaaa aggacagctg gactgtcaat gacatacaga
     3781 aattagtggg aaaattgaat tgggcaagtc agatttatgc agggattaaa gtaaggcaat
     3841 tatgtaaact tcttagggga accaaagcac taacagaagt agtaccacta acagaagaag
     3901 cagagctaga actggcagaa aacagggaga ttctaaaaga accggtacat ggagtgtatt
     3961 atgacccatc aaaagactta atagcagaaa tacagaagca ggggcaaggc caatggacat
     4021 atcaaattta tcaagagcca tttaaaaatc tgaaaacagg aaagtatgca agaatgaagg
     4081 gtgcccacac taatgatgtg aaacaattaa cagaggcagt acaaaaaata gccacagaaa
     4141 gcatagtaat atggggaaag actcctaaat ttaaattacc catacaaaag gaaacatggg
     4201 aagcatggtg gacagagtat tggcaagcca cctggattcc tgagtgggag tttgtcaata
     4261 cccctccctt agtgaagtta tggtaccagt tagagaaaga acccataata ggagcagaaa
     4321 ctttctatgt agatggggca gccaataggg aaactaaatt aggaaaagca ggatatgtaa
     4381 ctgacagagg aagacaaaaa gttgtccccc taacggacac aacaaatcag aagactgagt
     4441 tacaagcaat tcatctagct ttgcaggatt cgggattaga agtaaacata gtgacagact
     4501 cacaatatgc attgggaatc attcaagcac aaccagataa gagtgaatca gagttagtca
     4561 gtcaaataat agagcagtta ataaaaaagg aaaaagtcta cctggcatgg gtaccagcac
     4621 acaaaggaat tggaggaaat gaacaagtag ataaattggt cagtgctgga atcaggaaag
     4681 tactattttt agatggaata gataaggccc aagaagaaca tgagaaatat cacagtaatt
     4741 ggagagcaat ggctagtgat tttaacctac cacctgtagt agcaaaagaa atagtagcca
     4801 gctgtgataa atgtcagcta aaaggggaag ccatgcatgg acaagtagac tgtagcccag
     4861 gaatatggca gctagattgt acacatttag aaggaaaagt tatcttggta gcagttcatg
     4921 tagccagtgg atatatagaa gcagaagtaa ttccagcaga gacagggcaa gaaacagcat
     4981 acttcctctt aaaattagca ggaagatggc cagtaaaaac agtacataca gacaatggca
     5041 gcaatttcac cagtactaca gttaaggccg cctgttggtg ggcggggatc aagcaggaat
     5101 ttggcattcc ctacaatccc caaagtcaag gagtaataga atctatgaat aaagaattaa
     5161 agaaaattat aggacaggta agagatcagg ctgaacatct taagacagca gtacaaatgg
     5221 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     5281 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     5341 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg aaaggaccag
     5401 caaagctcct ctggaaaggt gaaggggcag tagtaataca agataatagt gacataaaag
     5461 tagtgccaag aagaaaagca aagatcatca gggattatgg aaaacagatg gcaggtgatg
     5521 attgtgtggc aagtagacag gatgaggatt aacacatgga aaagattagt aaaacaccat
     5581 atgtatattt caaggaaagc taaggactgg ttttatagac atcactatga aagtactaat
     5641 ccaaaaataa gttcagaagt acacatccca ctaggggatg ctaaattagt aataacaaca
     5701 tattggggtc tgcatacagg agaaagagac tggcatttgg gtcagggagt ctccatagaa
     5761 tggaggaaaa agagatatag cacacaagta gaccctgacc tagcagacca actaattcat
     5821 ctgcactatt ttgattgttt ttcagaatct gctataagaa ataccatatt aggacgtata
     5881 gttagtccta ggtgtgaata tcaagcagga cataacaagg taggatctct acagtacttg
     5941 gcactagcag cattaataaa accaaaacag ataaagccac ctttgcctag tgttaggaaa
     6001 ctgacagagg acagatggaa caagccccag aagaccaagg gccacagagg gagccataca
     6061 atgaatggac actagagctt ttagaggaac ttaagagtga agctgttaga cattttccta
     6121 ggatatggct ccataactta ggacaacata tctatgaaac ttacggggat acttgggcag
     6181 gagtggaagc cataataaga attctgcaac aactgctgtt tatccatttc agaattgggt
     6241 gtcgacatag cagaataggc gttactcgac agaggagagc aagaaatgga gccagtagat
     6301 cctagactag agccctggaa gcatccagga agtcagccta aaactgcttg taccaattgc
     6361 tattgtaaaa agtgttgctt tcattgccaa gtttgtttca tgacaaaagc cttaggcatc
     6421 tcctatggca ggaagaagcg gagacagcga cgaagagctc atcagaacag tcagactcat
     6481 caagcttctc tatcaaagca gtaagtagta catgtaatgc aacctataat agtagcaata
     6541 gtagcattag tagtagcaat aataatagca atagttgtgt ggtccatagt aatcatagaa
     6601 tataggaaaa tattaagaca aagaaaaata gacaggttaa ttgatagact aatagaaaga
     6661 gcagaagaca gtggcaatga gagtgaagga gaagtatcag cacttgtgga gatgggggtg
     6721 gaaatggggc accatgctcc ttgggatatt gatgatctgt agtgctacag aaaaattgtg
     6781 ggtcaccgtc tattatgggg tacctgtgtg gaaggaagca accaccactc tattttgtgc
     6841 atcagatgct aaagcatata tgatacagag gtacataatg tttgggccac acatgcctgt
     6901 gtacccacag accccaaccc acaagaagta gtattggtaa atgtgacaga aaattttaac
     6961 atgtggaaaa atgacatggt agaacagatg catgaggata taatcagttt atgggatcaa
     7021 agcctaaagc catgtgtaaa attaacccca ctctgtgtta gtttaaagtg cactgatttg
     7081 aagaatgata ctaataccaa tagtagtagc gggagaatga taatggagaa aggagagata
     7141 aaaaactgct ctttcaatat cagcacaagc ataagagata aggtgcagaa agaatatgca
     7201 ttcttttata aacttgatat agtaccaata gataatacca gctataggtt gataagttgt
     7261 aacacctcag tcattacaca ggcctgtcca aaggtatcct ttgagccaat tcccatacat
     7321 tattgtgccc cggctggttt tgcgattcta aaatgtaata ataagacgtt caatggaaca
     7381 ggaccatgta caaatgtcag cacagtacaa tgtacacatg gaatcaggcc agtagtatca
     7441 actcaactgc tgttaaatgg cagtctagca gaagaagatg tagtaattag atctgccaat
     7501 ttcacagaca atgctaaaac cataatagta cagctgaaca catctgtaga aattaattgt
     7561 acaagaccca acaacaatac aagaaaaagt atccgtatcc agaggggacc agggagagca
     7621 tttgttacaa taggaaaaat aggaaatatg agacaagcac attgtaacat tagtagagca
     7681 aaatggaatg ccactttaaa acagatagct agcaaattaa gagaacaatt tggaaataat
     7741 aaaacaataa tctttaagca atcctcagga ggggacccag aaattgtaac gcacagtttt
     7801 aattgtggag gggaattttt ctactgtaat tcaacacaac tgtttaatag tacttggttt
     7861 aatagtactt ggagtactga agggtcaaat aacactgaag gaagtgacac aatcacactc
     7921 ccatgcagaa taaaacaatt tataaacatg tggcaggaag taggaaaagc aatgtatgcc
     7981 cctcccatca gtggacaaat tagatgttca tcaaatatta ctgggctgct attaacaaga
     8041 gatggtggta ataacaacaa tgggtccgag atcttcagac ctggaggagg cgatatgagg
     8101 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     8161 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     8221 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     8281 ctgacggtac aggccagaca attattgtct gatatagtgc agcagcagaa caatttgctg
     8341 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caaacagctc
     8401 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     8461 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     8521 aaatctctgg aacagatttg gaataacatg acctggatgg agtgggacag agaaattaac
     8581 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     8641 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     8701 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     8761 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     8821 tttcagaccc acctcccaat cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     8881 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcctt agcacttatc
     8941 tgggacgatc tgcggagcct gtgcctcttc agctaccacc gcttgagaga cttactcttg
     9001 attgtaacga ggattgtgga acttctggga cgcagggggt gggaagccct caaatattgg
     9061 tggaatctcc tacaatattg gagtcaggaa ctaaagaata gtgctgttaa cttgctcaat
     9121 gccacagcca tagcagtagc tgaggggaca gatagggtta tagaagtagt acaaggagct
     9181 tgtagagcta ttcgccacat acctagaaga ataagacagg gcttggaaag gattttgcta
     9241 taagatgggt ggcgcggccg ctctggtgag caagggcgag gagctgttca ccggggtggt
     9301 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     9361 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     9421 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     9481 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     9541 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     9601 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     9661 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     9721 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     9781 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     9841 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     9901 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     9961 catggacgag ctgtacaagt aactcgagac ttacaaggca gctgtagatc ttagccactt
    10021 tttaaaagaa aaggggggac tggaagggct aactgcatcc ggagtacttc aagaactgct
    10081 gacatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
    10141 gcgggactgg ggagtggcga gccctcagat gctgcatata agcagctgct ttttgcctgt
    10201 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    10261 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    10321 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    10381 agcaggccgg cccaattgac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10441 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10501 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10561 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10621 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10681 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10741 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10801 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10861 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    10921 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10981 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    11041 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    11101 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    11161 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    11221 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    11281 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    11341 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    11401 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11461 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    11521 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    11581 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11641 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11701 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11761 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11821 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11881 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11941 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    12001 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    12061 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    12121 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    12181 cgcgcacatt tccccgaaaa gtgc
//
