LOCUS       sequence-194270-        3843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearraneavasa2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA
  TITLE     A premeiotic function for boule in the planarian Schmidtea 
            mediterranea.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 
            10.1073/pnas.1521341113. Epub 2016 Jun 2.
  PUBMED    27330085
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521341113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: 
            "E3509-18"
COMMENT     Sequence Label: vasa2
FEATURES             Location/Qualifiers
     source          1..3843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(722..740)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(723..740)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(744..762)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      776..823
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(835..852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1006..1594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1086..1105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1765..2556,2557..2625))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2388..2407
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2643..3437)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2754..2773)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3364..3383
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagaattaac cctcactaaa gggagacctt ttccaacgcg
      121 tgaattatgt cgccagatat ttaccgtggc gcatagactt tgtaaggatt cttccgtgaa
      181 atgcagctat tcttacggtg gcgttgaaat gagaacttcg atgatgaagt tgtataaatg
      241 cgatattctc gtgtcgactc ctgggagact tgtggccaat ctcgaatctg ctgcaatttc
      301 tctagataaa attcgatatt tagtgttgga tgaggcagat cggttactac agaatgaaaa
      361 atttatggaa tcgataaaac ttgtttatga aaaatctaaa ttagtcggag tcgataagtt
      421 ccatgtttcg atgttcagtg cgacgtttcc agctgaaata caacaactgg gcgaatcatt
      481 attgtctgat catttgtttg tcgctgtcgg attggttggt gcagccaata cagatattaa
      541 gcaaaccgtt ctacaagtca actctcatga caaactcaat gcttcattgg aatatttggc
      601 caataacaag accgggaaaa cattagtttt cgtgcaagca aagcgattcg ctgatttcct
      661 tgggattcga ttgattgatc tgaatttgct ggcaactaca atccatggcg acaaggtcac
      721 ttctatagtg tcacctaaat ctccctatag tgagtcgtat taggatccgg cgcgcctagc
      781 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgacgcgtg cttcctcgct
      841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1441 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     1801 gatctgtcta tttcgttcat ccatagttgc ctggctcccc gtcgtgtaga taactacgat
     1861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     1921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     2401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     2521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     2581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     2641 attcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     2701 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     2761 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
     2821 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     2881 acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc
     2941 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     3001 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     3061 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     3121 tgggatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     3181 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     3241 cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     3301 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     3361 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     3421 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc
     3481 ctgcgccatc agatcattgg cggcaagaaa gccatccagt ttactttgca gggcttccca
     3541 accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc
     3601 cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg
     3661 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc
     3721 ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga attttgttaa
     3781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcgacg
     3841 cgt
//
