LOCUS       sequence-194278-        4320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part 
            of GFP. Shows GFP localized to ribosomes when combined with GFP 
            1-10..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Noma K, Goncharov A, Ellisman MH, Jin Y
  TITLE     Microtubule-dependent ribosome localization in C. elegans neurons.
  JOURNAL   Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376.
  PUBMED    28767038
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-2"; volume: "6. pii: e26376. doi"; pages: " 
            10.7554/eLife.26376"
COMMENT     Sequence Label: pCZGY3163
FEATURES             Location/Qualifiers
     source          1..4320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(63..651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(143..162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(744..1535)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     1966..1982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1966..1982
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1987..2006
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1987..2005
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2023..2122
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2721..2768
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     protein_bind    complement(3653..3752)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3768..3785)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3768..3784)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3777..3799)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3848..3934
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4026..4053
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4212..4229)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
       61 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      661 ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgtaactcac gttaagggat
      721 tttggtcatg ggtggctcga gggttatttg ccgactacct tggtgatctc gcctttcacg
      781 tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca
      841 agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt
      901 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg
      961 cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat
     1021 agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt
     1081 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata
     1141 gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc
     1201 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc
     1261 acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt
     1321 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta
     1381 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa
     1441 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt
     1501 tgagtcgata cttcggcgat caccgcttcc ctcataatgt ttaactttgt tttagggcga
     1561 ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa
     1621 cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa
     1681 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     1741 ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac
     1801 tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggtagcagc
     1861 gaagtcgagg catttctgtc ctggctggtc tagaggaggc atgatgatat atttttatct
     1921 tgtgcaatgt aacatcagag attttgagac acgggccaga gctgccagga aacagctatg
     1981 accatgtaat acgactcact ataggggata tcagctggat ggcaaataat gattttattt
     2041 tgactgatag tgacctgttc gttgcaacaa attgatgagc aattattttt tataatgcca
     2101 actttgtaca agaaagctgg gtcgaattcg cccttccctc tactcaattt cagcaaaaat
     2161 attaatttaa ttcaccgaaa tctgtcagaa atcacgaaaa actttaattc ggactgcaag
     2221 gtctcgagac gattcaaagg cgcgtgcagt ttcgcggctg ggtttcgcac cgctcgtcct
     2281 ctctcctttc gtctctcttc ggaatttatt tcttttccct aaattttttt ggctttttcc
     2341 gttgaaactt taccaatttt cgcgattttt cttcaaaatt tgtttttgaa aaattcagga
     2401 taatttgttt tatcgatttt taaacattca aagctttgtt actcgacaaa ttcgctgaaa
     2461 acatgccgaa actcgaattt taatatttga aaattatcga atttactagt tttgtgaata
     2521 ttgtatcatt ttttaggcat ttacaggtca attttaactt attattcagc tgaaagtgct
     2581 taataataat gttttttggt aatttttcgt cgcaaatttt ttattttttt tgaatatccg
     2641 cgcttttcac tcgtgcatct cactaaattt tttatttttc agggtcataa aatcacgaca
     2701 agatgagatc tgccaccatg cgcgatcaca tggtcctgca tgagtatgtg aacgccgccg
     2761 ggatcactga gctcggatct ggatctggat ctggatctgg atcttctgca ggttcgttga
     2821 tcattccgga gaaattccag cacattcatc gtgtgatgaa caccaacatc gatggtaacc
     2881 gcaaggttcc atacgccttg accgccatca aggtttgttt tttaaatgtt tgatattttt
     2941 aatttttatc gttttcaggg agttggtcgt cgtttcgcct tcgtctgctg ccgcaaagct
     3001 gacgttgatg tcaacaagcg cgccggagag ctcactgagg aggacttcga caagattgtc
     3061 accatcatgc aaaacccatc tcaatacaaa atcccaaatt ggttccttaa cagacaaaag
     3121 gacatcaagg acggaaagac tggacaggtt agttgtttcg tctaaaaaaa atcacttaaa
     3181 attatacaac tggagtgaaa attaggcctg tttttttttt aaatctgtat acaggccacc
     3241 cagtttaatt attttgagag tagcaaattt tgactataat tcttatagac atatgttttc
     3301 agctcctctc caccgccgtc gacaacaagc tccgtgagga tcttgagcgc atgaagaaga
     3361 tccgtctcca cagaggactc agacattact ggggacttcg cgtcagagga cagcacacca
     3421 agaccaccgg acgcaaggga cgcaccgtcg gagtgtccaa gaagaaggga ggataagtct
     3481 gattgtttac gggatgttat gttatttttt gttgctagta tcccgaaaat aaatgttact
     3541 ttcaaaaata atttctttat ttgtcaaaca atacaagttt taaaccattt tcttgtgttt
     3601 ttggtagaat tcaagatcga ggcaaacatt attaaagaaa gggcgaattc ggagcctgct
     3661 tttttgtaca aagttggcat tataaaaaag cattgctcat caatttgttg caacgaacag
     3721 gtcactatca gtcaaaataa aatcattatt tggggcccga gcttaagact ggccgtcgtt
     3781 ttacaacgtc gtgactggga aaacatccat gctagcgtta acgcgagagt agggaactgc
     3841 caggcatcaa ataaaacgaa aggctcagtc ggaagactgg gcctttcgtt ttatctgttg
     3901 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgt
     3961 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaac
     4021 taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc ttcctggcta
     4081 gcggtacgcg tattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4141 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4201 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4261 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
//
