LOCUS       sequence-194280-        7334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""Activate neurons with 2p stimulation"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7334)
  TITLE     AAV plasmids expressing Red-shifted channelrhodopsins with soma 
            localization sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CamKIIa-C1V1::FusionRed::Kv2.1
FEATURES             Location/Qualifiers
     source          1..7334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        160..1448
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     misc_feature    3461..4049
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3514..3534)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3932..3943)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4081..4557
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4280..4299)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4597..4737
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4597..4726
                     /label=AAV2 ITR
     rep_origin      4812..5267
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4899..4918)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5109..5130
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5284..5303)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5403..5425
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5463..5481)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5549..5653
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5654..5722,5723..6514)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5872..5891)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6685..7273
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7174..7193
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
      181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
      241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
      301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
      361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
      421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
      481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
      541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
      601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
      661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
      721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
      781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
      841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
      901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
      961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
     1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
     1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
     1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
     1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
     1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
     1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
     1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
     1441 ggggcagctc tagagcggta ccggatcccc gggtaccggt gccaccatga gtcggcgacc
     1501 ctggcttctc gcacttgctt tggctgtagc tttggcagct ggaagtgctg gggctagtac
     1561 cgggtctgac gctactgtcc ctgtggccac ccaggacgga ccagattatg tcttccatag
     1621 agcccacgag agaatgcttt tccagactag ctataccctt gaaaataacg gtagcgtaat
     1681 ttgcattccc aataacggcc agtgtttctg cctggcttgg ttgaagtcta acggcacaaa
     1741 cgccgaaaag ctggctgcaa atatacttca atggattacc ttcgcattga gtgcattgtg
     1801 tctcatgttc tatggttacc aaacctggaa gagcacttgc gggtgggaga ctatctacgt
     1861 tgctacaata gaaatgataa agttcataat tgaatacttc catgaatttg atgaacctgc
     1921 cgtcatttac agttcaaatg gtaataaaac cgtttggttg agatacgcaa catggctgct
     1981 gacttgcccc gtcttgctta ttcatctgag taatctgact ggtctgaagg acgactattc
     2041 taaacgaaca atggggctcc tcgtcagtga tgtgggctgt atcgtctggg gcgcaaccag
     2101 cgctatgtgt accgggtgga caaaaatact gttcttcctt attagcctgt cttatgggat
     2161 gtacacctac ttccacgctg ctaaagtcta tatcgaagcc tttcacactg tccccaaggg
     2221 catatgtcgg gaactggtaa gggtgatggc ttggaccttc tttgttgctt ggggcatgtt
     2281 ccctgttttg tttctgttgg gcaccgaggg atttggtcat atttctccat atggaagcgc
     2341 tatcggccat tcaatccttg atctcatagc taaaaacatg tggggtgtgc tggggaatta
     2401 cttgcgagtt aaaattcatg agcatattct tctttatggc gacataagaa aaaaacaaaa
     2461 gatcactatt gcagggcaag aaatggaggt cgaaaccctt gttgcagagg aggaggacgc
     2521 cgctccagta gtagctgtga gcgaactcat aaaggagaat atgcctatga aactttacat
     2581 ggaagggacc gtaaacaatc atcacttcaa atgtacttcc gaaggtgagg gtaaacctta
     2641 tgaaggcaca caaacaatga ggattaaggt ggttgaaggt ggccccttgc cattcgcttt
     2701 tgacattttg gcaacaagct tcatgtacgg ctcacgaaca tttattaaac acccccccgg
     2761 tattcctgac ttctttaagc aaagcttccc tgagggtttt acatgggaac gggtaaccac
     2821 ctacgaggac ggcggcgttc tgacagcaac acaagacaca agtctgcagg acgggtgtct
     2881 catctacaat gtgaaagtaa gaggtgtcaa tttccccgct aacggtcctg taatgcaaaa
     2941 gaaaacactc gggtgggagg cttccacaga aacaatgtat ccagccgacg gaggcttgga
     3001 gggggcctgc gacatggcct tgaagcttgt agggggaggg catctcatat gtaacctcga
     3061 gacaacttac aggagcaaga aacccgccac taatttgaag atgcccgggg tctacaacgt
     3121 tgatcaccgg cttgagcgaa tcaaagaggc tgacgacgaa acctatgtag aacaacacga
     3181 agttgccgtg gcccgatata gcaccggggg tgccggtgat ggtgggaagt acaaacaaag
     3241 ccagcccatc cttaacacta aagagatggc tccacaatca aaaccacccg aggagcttga
     3301 aatgagctcc atgccttctc ctgtagctcc cctccccgca agaacagaag gggtgattga
     3361 catgcgctct atgagcagta tagacagctt catctcatgt gctaccgact tcccagaagc
     3421 aacaaggttt aagtaagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     3481 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3541 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3601 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3661 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3721 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3781 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3841 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3901 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3961 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     4021 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac
     4081 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     4141 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     4201 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     4261 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     4321 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     4381 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     4441 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     4501 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     4561 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     4621 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     4681 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     4741 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     4801 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4861 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4921 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4981 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     5041 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5101 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     5161 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5221 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     5281 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5341 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5401 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     5461 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5521 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5581 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5641 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5701 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5761 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5821 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5881 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5941 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6001 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6061 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6121 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6181 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6241 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6301 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6361 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6421 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6481 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6541 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6601 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6661 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6721 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6781 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6841 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6901 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6961 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7021 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7081 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7141 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7201 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7261 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7321 ttttgctcac atgt
//
