LOCUS       sequence-194296-        7180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Oliveira CR, Lemaitre R, Murawala P, Tazaki A, Drechsel DN, Tanaka 
            EM
  TITLE     Pseudotyped baculovirus is an effective gene expression tool for 
            studying molecular function during axolotl limb regeneration.
  JOURNAL   Dev Biol. 2018 Jan 15;433(2):262-275. doi: 
            10.1016/j.ydbio.2017.10.008. Epub 2017 Nov 30.
  PUBMED    29198566
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2017.10.008"; journalName: "Dev Biol"; date: 
            "2018-01-15- 15"; volume: "433"; issue: "2"; pages: "262-275"
COMMENT     Sequence Label: pOEM-GED-EGFP
FEATURES             Location/Qualifiers
     source          1..7180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     692..709
                     /label=L4440
                     /note="L4440 vector, forward primer"
     3'UTR           1061..1433
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        1534..1837
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1838..2041
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1992..2012
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2080..2089
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2086..2088,2089..2091,2092..2802)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2086..2088,2089..2091,2092..2802)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2131..2152)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2392..2411)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2739..2760
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2878..2898)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3296..3307)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3542..3676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3579..3598)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3633..3652
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3789..3821)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(4017..4076)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(4139..4230)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4197..4214)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4316..4921
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(4959..5083)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(5693..5712)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5694..5712)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5723..5740)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5723..5739)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5732..5754)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        6055..6159
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6160..6228,6229..7020)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6378..6397)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcattagg
      661 caccccaggc tttacccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggagag
      721 cgcccaatac gcaaggaaac agctatgacc atgttaatgc agctggcacg acaggtttcc
      781 cgactggaaa gcgggcagtg agcggaaggc ccatgaggcc caaaaagctc aactacaaat
      841 ttgatcgcgg acgtgttagc cgacacaatt aataggcgtc gtgtggctat ggcaaaatcg
      901 tcttcggaag caacttctaa cgacgagggt tgggacgacg acgataatcg gcctaataaa
      961 gctaacacgc ccgatgttaa atatgtccaa gcgttattta acgtgtttac gtcgagtcaa
     1021 ttgtacacta acgacagtga tgaaagaaat acaaaagcgc ataatatttt gaacgacgtc
     1081 gaacctttat tacaaaacaa aacacaaacg aatatcgaca aagctagatt gctgctacaa
     1141 gatttggcaa gttttgtggc gttgagcgaa aatccattag atagtccagc catcggttcg
     1201 gaaaaacaac ccttgtttga aactaatcga aacctatttt acaaatctat tgaggattta
     1261 atatttaaat tcagatataa agacgctgaa aatcatttga ttttcgctct aacataccac
     1321 cctaaagatt ataaatttaa tgaattatta aaatacgtac aacaattgtc tgtaaatcaa
     1381 caacgcacag aatctagcgc ttaataaatg tactaataac aatgtatcgt gttttattaa
     1441 ttaagatctg cggatgcagg ctccgccatg cattagttat taatagtaat caattacggg
     1501 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1561 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1621 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1681 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1741 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1801 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     1861 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     1921 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     1981 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2041 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
     2101 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2161 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2221 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2281 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     2341 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2401 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2461 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2521 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     2581 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2641 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2701 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2761 accgccgccg ggatcactct cggcatggac gagctgtaca agtgcggccg ctagacgcgt
     2821 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2881 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2941 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3001 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3061 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3121 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3181 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     3241 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3301 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3361 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgaagcttgt
     3421 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3481 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3541 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3601 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3661 ttatcatgtc tggatctgat cactggcgcg ccgtgatgcg tatttctgaa tctttgtaaa
     3721 atagcacaca agactccaac gcgtttggcg ttttattttc ttgctcgact ctagtttatt
     3781 aggccttact ttccaagtcg gttcatctct atgtctgtat aaatctgtct tttcttggtg
     3841 tgctttaatt taatgcaaag atggatacca actcggagaa ccaagaatag tccaatgatt
     3901 aaccctatga taaagaaaaa agaggcaata gagcttttcc aactactgaa ccaaccttct
     3961 acaagctcga ttggattttt ggatagccca gtatcacccc ggggtacctg cagatccgca
     4021 aaggcagaat gcgccgccgc cgccaaaagc acatataaaa caatagcgct taccatcttg
     4081 cttgtgtgtt ccttattgaa gccttggtgt gactgattta ctagtagcat agatccgtat
     4141 ttataggttt ttttattaca aaactgttac gaaaacagta aaatacttat ttatttgcga
     4201 gatggttatc attttaatta tctccatgat ccagccaacc actagaacta tagctagagt
     4261 cctgggcgaa caaaatggtg gcagcttgtg atattagttt gtgcgtctca ttacaatggc
     4321 tgttattttt aacaacaaac aactgctcgc agacaatagt atagaaaagg gaggtgaact
     4381 gtttttgttt aacggttcgt acaacatttt ggaaagttat gttaatccgg tgctgctaaa
     4441 aaatggtgta attgaactag aagaagctgc gtactatgcc ggcaacatat tgtacaaaac
     4501 cgacgatccc aaattcattg attatataaa tttaataatt aaagcaacac actccgaaga
     4561 actaccagaa aatagcactg ttgtaaatta cagaaaaact atgcgcagcg gtactataca
     4621 ccccattaaa aaagacatat atatttatga caacaaaaaa tttactctat acgatagata
     4681 catatatgga tacgataata actatgttaa tttttatgag gagaaaaatg aaaaagagaa
     4741 ggaatacgaa gaagaagacg acaaggcgtc tagtttatgt gaaaataaaa ttatattgtc
     4801 gcaaattaac tgtgaatcat ttgaaaatga ttttaaatat tacctcagcg attataacta
     4861 cgcgttttca attatagata atactacaaa tgttcttgtt gcgtttggtt tgtatcgtta
     4921 ataaaaaaca aatttagcat ttataattgt tttattattc aataattaca aataggattg
     4981 agacccttgc agttgccagc aaacggacag agcttgtcca cagttttcat tttttggcag
     5041 tttggtggtt tatacacatc ttgggatttg attaaaagat gcatgacttc acccacacac
     5101 ttggagtcgt ggttgtagaa atgcgtcaga gccttgatta aacatcggtt ttcacacttg
     5161 gcacacgaaa ccacgtttgc aatgtaacag tttagtataa acctcttcct gtacataccg
     5221 ccgcgcggta cattgttttc tttcaaatcg ttcaatattt tttttataca cggaggcata
     5281 ttaatcttgt tgttcagcat gtccacgaca tttttctttc cttctacgca ttctttcata
     5341 caaatgtttc tcttgctgcg ttttttaaaa ttggaatttt taatttcatt aatagtgggc
     5401 ggagccgtta acagcatgta caatcgcagt ccggaaattt tgaccaaaac cccgccattg
     5461 tagaataccg tgtatggagt tagggtcaat ttatcaataa aatcatcggg atcaattaaa
     5521 tatgtagctt tcttgtctaa acatgacgct ctaatgagcg gactccacac gttatacgat
     5581 gcattcgcca tacttcgcgg cttctcgcac cagcggtcct gattcgacta acggtcgatt
     5641 ggatggctta atagttttaa cgtcacggcc ttgacggcct tccgccaatt cgccctatag
     5701 tgagtcgtat tacgtcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     5761 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5821 gcgaagaggc ccgcaccgaa acgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5881 gagcgccctg tagcggccac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5941 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6001 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     6061 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6121 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6181 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6241 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6301 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6361 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6421 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6481 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6541 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6601 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6661 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6721 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6781 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6841 tttattgctg ataaatctgg agccggtgag cgtggttctc gcggtatcat tgcagcactg
     6901 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6961 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7021 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7081 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7141 ttttcgttcc actgagcgtc agaccccgta gaaaagatca
//
