LOCUS       sequence-194298-        7179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Oliveira CR, Lemaitre R, Murawala P, Tazaki A, Drechsel DN, Tanaka 
            EM
  TITLE     Pseudotyped baculovirus is an effective gene expression tool for 
            studying molecular function during axolotl limb regeneration.
  JOURNAL   Dev Biol. 2018 Jan 15;433(2):262-275. doi: 
            10.1016/j.ydbio.2017.10.008. Epub 2017 Nov 30.
  PUBMED    29198566
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2017.10.008"; journalName: "Dev Biol"; date: 
            "2018-01-15- 15"; volume: "433"; issue: "2"; pages: "262-275"
COMMENT     Sequence Label: pOEM-GED-mCherry
FEATURES             Location/Qualifiers
     source          1..7179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     692..709
                     /label=L4440
                     /note="L4440 vector, forward primer"
     3'UTR           1061..1433
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        1534..1837
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1838..2041
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1992..2012
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2080..2089
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2086..2796
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2231..2249)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2499..2518
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2877..2897)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3295..3306)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3541..3675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3578..3597)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3632..3651
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3788..3820)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(4016..4075)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(4138..4229)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4196..4213)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4315..4920
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(4958..5082)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(5692..5711)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5693..5711)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5722..5739)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5722..5738)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5731..5753)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        6054..6158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6159..6227,6228..7019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6377..6396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcattagg
      661 caccccaggc tttacccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggagag
      721 cgcccaatac gcaaggaaac agctatgacc atgttaatgc agctggcacg acaggtttcc
      781 cgactggaaa gcgggcagtg agcggaaggc ccatgaggcc caaaaagctc aactacaaat
      841 ttgatcgcgg acgtgttagc cgacacaatt aataggcgtc gtgtggctat ggcaaaatcg
      901 tcttcggaag caacttctaa cgacgagggt tgggacgacg acgataatcg gcctaataaa
      961 gctaacacgc ccgatgttaa atatgtccaa gcgttattta acgtgtttac gtcgagtcaa
     1021 ttgtacacta acgacagtga tgaaagaaat acaaaagcgc ataatatttt gaacgacgtc
     1081 gaacctttat tacaaaacaa aacacaaacg aatatcgaca aagctagatt gctgctacaa
     1141 gatttggcaa gttttgtggc gttgagcgaa aatccattag atagtccagc catcggttcg
     1201 gaaaaacaac ccttgtttga aactaatcga aacctatttt acaaatctat tgaggattta
     1261 atatttaaat tcagatataa agacgctgaa aatcatttga ttttcgctct aacataccac
     1321 cctaaagatt ataaatttaa tgaattatta aaatacgtac aacaattgtc tgtaaatcaa
     1381 caacgcacag aatctagcgc ttaataaatg tactaataac aatgtatcgt gttttattaa
     1441 ttaagatctg cggatgcagg ctccgccatg cattagttat taatagtaat caattacggg
     1501 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1561 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1621 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1681 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1741 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1801 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     1861 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     1921 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     1981 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2041 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
     2101 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     2161 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2221 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     2281 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     2341 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2401 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     2461 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     2521 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2581 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2641 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2701 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2761 cactccaccg gcggcatgga cgagctgtac aagtaagaat tcgcggccgc tagacgcgtc
     2821 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2881 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2941 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3001 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3061 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3121 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3181 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     3241 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3301 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3361 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gaagcttgtc
     3421 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     3481 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     3541 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3601 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3661 tatcatgtct ggatctgatc actggcgcgc cgtgatgcgt atttctgaat ctttgtaaaa
     3721 tagcacacaa gactccaacg cgtttggcgt tttattttct tgctcgactc tagtttatta
     3781 ggccttactt tccaagtcgg ttcatctcta tgtctgtata aatctgtctt ttcttggtgt
     3841 gctttaattt aatgcaaaga tggataccaa ctcggagaac caagaatagt ccaatgatta
     3901 accctatgat aaagaaaaaa gaggcaatag agcttttcca actactgaac caaccttcta
     3961 caagctcgat tggatttttg gatagcccag tatcaccccg gggtacctgc agatccgcaa
     4021 aggcagaatg cgccgccgcc gccaaaagca catataaaac aatagcgctt accatcttgc
     4081 ttgtgtgttc cttattgaag ccttggtgtg actgatttac tagtagcata gatccgtatt
     4141 tataggtttt tttattacaa aactgttacg aaaacagtaa aatacttatt tatttgcgag
     4201 atggttatca ttttaattat ctccatgatc cagccaacca ctagaactat agctagagtc
     4261 ctgggcgaac aaaatggtgg cagcttgtga tattagtttg tgcgtctcat tacaatggct
     4321 gttattttta acaacaaaca actgctcgca gacaatagta tagaaaaggg aggtgaactg
     4381 tttttgttta acggttcgta caacattttg gaaagttatg ttaatccggt gctgctaaaa
     4441 aatggtgtaa ttgaactaga agaagctgcg tactatgccg gcaacatatt gtacaaaacc
     4501 gacgatccca aattcattga ttatataaat ttaataatta aagcaacaca ctccgaagaa
     4561 ctaccagaaa atagcactgt tgtaaattac agaaaaacta tgcgcagcgg tactatacac
     4621 cccattaaaa aagacatata tatttatgac aacaaaaaat ttactctata cgatagatac
     4681 atatatggat acgataataa ctatgttaat ttttatgagg agaaaaatga aaaagagaag
     4741 gaatacgaag aagaagacga caaggcgtct agtttatgtg aaaataaaat tatattgtcg
     4801 caaattaact gtgaatcatt tgaaaatgat tttaaatatt acctcagcga ttataactac
     4861 gcgttttcaa ttatagataa tactacaaat gttcttgttg cgtttggttt gtatcgttaa
     4921 taaaaaacaa atttagcatt tataattgtt ttattattca ataattacaa ataggattga
     4981 gacccttgca gttgccagca aacggacaga gcttgtccac agttttcatt ttttggcagt
     5041 ttggtggttt atacacatct tgggatttga ttaaaagatg catgacttca cccacacact
     5101 tggagtcgtg gttgtagaaa tgcgtcagag ccttgattaa acatcggttt tcacacttgg
     5161 cacacgaaac cacgtttgca atgtaacagt ttagtataaa cctcttcctg tacataccgc
     5221 cgcgcggtac attgttttct ttcaaatcgt tcaatatttt ttttatacac ggaggcatat
     5281 taatcttgtt gttcagcatg tccacgacat ttttctttcc ttctacgcat tctttcatac
     5341 aaatgtttct cttgctgcgt tttttaaaat tggaattttt aatttcatta atagtgggcg
     5401 gagccgttaa cagcatgtac aatcgcagtc cggaaatttt gaccaaaacc ccgccattgt
     5461 agaataccgt gtatggagtt agggtcaatt tatcaataaa atcatcggga tcaattaaat
     5521 atgtagcttt cttgtctaaa catgacgctc taatgagcgg actccacacg ttatacgatg
     5581 cattcgccat acttcgcggc ttctcgcacc agcggtcctg attcgactaa cggtcgattg
     5641 gatggcttaa tagttttaac gtcacggcct tgacggcctt ccgccaattc gccctatagt
     5701 gagtcgtatt acgtcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     5761 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5821 cgaagaggcc cgcaccgaaa cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     5881 agcgccctgt agcggccact caaccctatc tcggtctatt cttttgattt ataagggatt
     5941 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6001 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     6061 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6121 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6181 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6241 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6301 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6361 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6421 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6481 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6541 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6601 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6661 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6721 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6781 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6841 ttattgctga taaatctgga gccggtgagc gtggttctcg cggtatcatt gcagcactgg
     6901 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6961 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7021 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7081 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7141 tttcgttcca ctgagcgtca gaccccgtag aaaagatca
//
