LOCUS       sequence-194300-       12773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Please see depositor comments section..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12773)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 12773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pAGM4723
FEATURES             Location/Qualifiers
     source          1..12773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             351..1424
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1490..1684
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        1746..1850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1851..2645
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2816..3404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3305..3324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3540..3564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      9748..9757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    11762..11786
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12066..12695
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
       61 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
      121 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
      181 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
      241 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
      301 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
      361 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
      421 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
      481 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
      541 cgtcgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
      601 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
      661 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
      721 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
      781 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
      841 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
      901 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
      961 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     1021 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     1081 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     1141 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     1201 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     1261 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     1321 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     1381 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     1441 tagggcaaat tgccctagca ggggaaaaag gtcgaaaaag cttctttcct gtggatagca
     1501 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     1561 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     1621 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     1681 cataactgtc tggccagcgc acagccgaac agctgcaaaa agcgcccaca tcaaggctcc
     1741 gagtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1801 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atggctaaaa
     1861 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     1921 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     1981 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     2041 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     2101 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     2161 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     2221 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     2281 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaggacactc
     2341 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     2401 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     2461 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     2521 gcgtccggtc gctcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     2581 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     2641 tttagctgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2701 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2761 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2821 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2881 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2941 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3001 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3061 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3421 cctttttacg gttcctgctc ggatctgttg gaccggacag tagtcatggt tgatgggctg
     3481 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     3541 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
     3601 acgtttttaa tgtactgggg ttgaacactc tgtgccttgt cttccagtgg tatggggtac
     3661 cgcacggtct gccaatccga cggaggttta tgaattttcc accttttcca caagctcaac
     3721 tagtattaac gatgtggatt tagcaaaaaa aacctgtaac cctaaatgta aaataacggg
     3781 taagcctgcc aaccatgtta tggcagatta agcgtctttt tgaagggcac cgcatctttc
     3841 gcgttgccgt aaatgtatcc gtttagcaat aaggaaattc ttaatgaata atccgtcgtt
     3901 actcaatcat gcggtcgaaa cgatggcagt tggctcgaaa agttttgcga cagcctcaaa
     3961 gttatttgat gcaaaaaccc ggcgcagcgt actgatgctc tacgcctggt gccgccattg
     4021 tgacgatgtt attgacgatc agacgctggg ctttcaggcc cggcagcctg ccttacaaac
     4081 gcccgaacaa cgtctgatgc aacttgagat gaaaacgcgc caggcctatg caggatcgca
     4141 gatgcacgaa ccggcgtttg cggcttttcg ggaagtggct atggctcatg atatcgcccc
     4201 ggcttacgcg tttgatcatc tggaaggctt cgccatggat gtacgcgaag cgcaatacag
     4261 ccaactggat gatacgctgc gctattgcta tcacgttgca ggcgttgtcg gcttgatgat
     4321 ggcgcaaatc atgggcgtgc gggataacgc cacgctggac cgcgcctgtg accttgggct
     4381 ggcatttcag ttgaccaata ttgctcgcga tattgtggac gatgcgcatg cgggccgctg
     4441 ttatctgccg gcaagctggc tggagcatga aggtctgaac aaagagaatt atgcggcacc
     4501 tgaaaaccgt caggcgctga gccgtatcgc ccgtcgtttg gtgcaggaag cagaacctta
     4561 ctatttgtct gccacagccg gcctggcagg gttgcccctg cgttccgcct gggcaatcgc
     4621 tacggcgaag caggtttacc ggaaaatagg tgtcaaagtt gaacaggccg gtcagcaagc
     4681 ctgggatcag cggcagtcaa cgaccacgcc cgaaaaatta acgctgctgc tggccgcctc
     4741 tggtcaggcc cttacttccc ggatgcgggc tcatcctccc cgccctgcgc atctctggca
     4801 gcgcccgctc taggcttact ataaggatgt ttgcaatgaa accaactacg gtaattggtg
     4861 caggcttcgg tggcctggca ctggcaattc gtctacaagc tgcggggatc cccgtcttac
     4921 tgcttgaaca acgtgataaa cccggcggtc gggcttatgt ctacgaggat caggggttta
     4981 cctttgatgc aggcccgacg gttatcaccg atcccagtgc cattgaagaa ctgtttgcac
     5041 tggcaggaaa acagttaaaa gagtatgtcg aactgctgcc ggttacgccg ttttaccgcc
     5101 tgtgttggga gtcagggaag gtctttaatt acgataacga tcaaacccgg ctcgaagcgc
     5161 agattcagca gtttaatccc cgcgatgtcg aaggttatcg tcagtttctg gactattcac
     5221 gcgcggtgtt taaagaaggc tatctaaagc tcggtactgt ccctttttta tcgttcagag
     5281 acatgcttcg cgccgcacct caactggcga aactgcaggc atggagaagc gtttacagta
     5341 aggttgccag ttacatcgaa gatgaacatc tgcgccaggc gttttctttc cactcgctgt
     5401 tggtgggcgg caatcccttc gccacctcat ccatttatac gttgatacac gcgctggagc
     5461 gtgagtgggg cgtctggttt ccgcgtggcg gcaccggcgc attagttcag gggatgataa
     5521 agctgtttca ggatctgggt ggcgaagtcg tgttaaacgc cagagtcagc catatggaaa
     5581 cgacaggaaa caagattgaa gccgtgcatt tagaggacgg tcgcaggttc ctgacgcaag
     5641 ccgtcgcgtc aaatgcagat gtggttcata cctatcgcga cctgttaagc cagcaccctg
     5701 ccgcggttaa gcagtccaac aaactgcaga ctaagcgcat gagtaactct ctgtttgtgc
     5761 tctattttgg tttgaatcac catcatgatc agctcgcgca tcacacggtt tgtttcggcc
     5821 cgcgttaccg cgagctgatt gacgaaattt ttaatcatga tggcctcgca gaggacttct
     5881 cactttatct gcacgcgccc tgtgtcacgg attcgtcact ggcgcctgaa ggttgcggca
     5941 gttactatgt gttggcgccg gtgccgcatt taggcaccgc gaacctcgac tggacggttg
     6001 aggggccaaa actacgcgac cgtatttttg cgtaccttga gcagcattac atgcctggct
     6061 tacggagtca gctggtcacg caccggatgt ttacgccgtt tgattttcgc gaccagctta
     6121 atgcctatca tggctcagcc ttttctgtgg agcccgttct tacccagagc gcctggtttc
     6181 ggccgcataa ccgcgataaa accattacta atctctacct ggtcggcgca ggcacgcatc
     6241 ccggcgcagg cattcctggc gtcatcggct cggcaaaagc gacagcaggt ttgatgctgg
     6301 aggatctgat atgagctttt acccaggaga cagagaatgc aaccgcatta tgatctgatt
     6361 ctcgtggggg ctggactcgc gaatggcctt atcgccctgc gacttcagca gcagcaacct
     6421 gatatgcgta ttttgcttat cgacgccgca ccccaggcgg gcgggaatca tacgtggtca
     6481 tttcaccacg atgatttgac tgagagccaa catcgttgga tagctccgct ggtggttcat
     6541 cactggcccg actatcaggt acgctttccc acacgccgtc gtaagctgaa cagcggctac
     6601 ttttgtatta cttctcagcg tttcgctgag gttttacagc gacagtttgg cccgcacttg
     6661 tggatggata ccgcggtcgc agaggttaat gcggaatctg ttcggttgaa aaagggtcag
     6721 gttatcggtg cccgcgcggt gattgacggg cggggttatg cggcaaattc agcactgagc
     6781 gtgggcttcc aggcgtttat tggccaggaa tggcgattga gtcacccgca tggtttatca
     6841 tctccgatta tcatggatgc cacggtcgat cagcaaaatg gttatcgctt cgtgtacagc
     6901 ctgccgctct cgccgaccag attgttaatt gaagatacgc actatattga taatgcgaca
     6961 ttagatcctg aatgcgcgcg gcaaaatatt tgcgactatg ccgcgcaaca gggttggcag
     7021 cttcagacac tgctgcgaga agaacagggc gccttaccca ttactctgtc gggcaatgcc
     7081 gacgcattct ggcagcagcg ccccctggcc tgtagtggat tacgtgccgg tctgttccat
     7141 cctaccaccg gctattcact gccgctggcg gttgccgtgg ccgaccgcct gagtgcactt
     7201 gatgtcttta cgtcggcctc aattcaccat gccattacgc attttgcccg cgagcgctgg
     7261 cagcagcagg gctttttccg catgctgaat cgcatgctgt ttttagccgg acccgccgat
     7321 tcacgctggc gggttatgca gcgtttttat ggtttacctg aagatttaat tgcccgtttt
     7381 tatgcgggaa aactcacgct gaccgatcgg ctacgtattc tgagcggcaa gccgcctgtt
     7441 ccggtattag cagcattgca agccattatg acgactcatc gttaagcttc agaggaggaa
     7501 ttaagtaatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     7561 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     7621 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     7681 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     7741 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     7801 ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     7861 aagcgtcggg cattcatcaa cctccattag tgccggaatt ggtattgcgg ttgctgccga
     7921 aaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgcagg
     7981 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtgattct
     8041 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaacaacc atctggcaca
     8101 gctgctttcc ggtaagcttt actcttcact gcgcgaaggc gggaaaaaag ttttctctgg
     8161 cgtgccgcca attaaagagc tgctcaaacg caccgaagaa catattaaag gcatggtagt
     8221 gcctggcacg ttgtttgagg agctgggctt taactacatc ggcccggtgg acggtcatga
     8281 tgtgctgggg cttatcacca cgctaaagaa catgcgcgac ctgaaaggcc cgcagttcct
     8341 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc cgatcacttt
     8401 ccatgccgtg ctaaatttga tccctccagc ggttgtttgc cgaaaagtag cggcggtttg
     8461 ccgagctatt caaaaatctt tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag
     8521 ctgatggcga ttactccggc gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa
     8581 ttcccggatc gctacttcga cgtggcaatt gccgagcaac atgcggtgac ctttgctgcg
     8641 ggtctggcga ttggtgggta caaacccatt gtcgcgattt actccacttt cctgcaacgc
     8701 gcctatgatc aggtgctgca tgacgtggcg attcaaaagc ttccggtcct gttcgccatc
     8761 gaccgcgcgg gcattgttgg tgctgacggt caaacccatc agggtgcttt tgatctctct
     8821 tacctgcgct gcataccgga aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc
     8881 cagatgctct ataccggcta tcactataac gatggcccgt cagcggtgcg ctacccgcgt
     8941 ggcaacgcgg tcggcgtgga actgacgccg ctggaaaaac taccaattgg caaaggcatt
     9001 gtgaagcgtc gtggcgagaa actggcgatc cttaactttg gtacgctgat gccagaagcg
     9061 gcgaaagtcg ccgaatcgct gaacgccacg ctggtcgata tgcgttttgt gaaaccgctt
     9121 gatgaagcgt taattctgga aatggccgcc agccatgaag cgctggtcac cgtagaagaa
     9181 aacgccatta tgggcggcgc aggcagcggc gtgaacgaag tgctgatggc ccatcgtaaa
     9241 ccagtacccg tgctgaacat tggcctgccg gacttcttta ttccgcaagg aactcaggaa
     9301 gaaatgcgcg ccgaactcgg cctcgatgcc gctggtatgg aagccaaaat caaggcctgg
     9361 ctggcataag ctttgtgtga ggagatgtcg aatgagcgca catgccctgc ccaaggcaga
     9421 tctgaccgcc accagcctga tcgtatcggg cggcatcatc gccgcttggc tggccctgca
     9481 tgtgcatgcg ctgtggtttc tggacgcagc ggcgcatccc atcctggcga tcgcaaattt
     9541 cctggggctg acctggctgt cggtcggatt gttcatcatc gcgcatgacg cgatgcacgg
     9601 gtcggtggtg ccggggcgtc cgcgcgccaa tgcggcgatg ggccagcttg tcctgtggct
     9661 gtatgccgga ttttcgtggc gcaagatgat cgtcaagcac atggcccatc accgccatgc
     9721 cggaaccgac gacgaccccg atttcgacca tggcggcccg gtccgctggt acgcccgctt
     9781 catcggcacc tatttcggct ggcgcgaggg gctgcttctg cccgtcatcg tgacggtcta
     9841 tgcgctgatc cttggggatc gctggatgta cgtggtattc tggccgctgc cgtcgatcct
     9901 ggcgtcgatc cagctgttcg tgttcggcac ctggctgccg caccgccccg gccacgacgc
     9961 gttcccggac cgccacaatg cgcggtcgtc gcggatcagc gaccccgtgt cgctgctgac
    10021 ctgttttcac tttggcggtt atcatcacga acaccacctg cacccgacgg tgccgtggtg
    10081 gcgcctgccc agcacccgca ccaagggcga caccgcataa gcttgagctg aggagatttt
    10141 gaatgcaaac ggaacacgtc attttattga atgcacaggg agttcccacg ggtacgctgg
    10201 aaaagtatgc cgcacacacg gcagacaccc gcttacatct cgcgttctcc agttggctgt
    10261 ttaatgccaa aggacaatta ttagttaccc gccgcgcact gagcaaaaaa gcatggcctg
    10321 gcgtgtggac taactcggtt tgtgggcacc cacaactggg agaaagcaac gaggacgcag
    10381 tgatccgccg ttgccgttat gagcttggcg tggaaattac gcctcctgaa tctatctatc
    10441 ctgactttcg ctaccgcgcc accgatccga gtggcattgt ggaaaatgaa gtgtgtccgg
    10501 tatttgccgc acgcaccact agtgcgttac agatcaatga tgatgaagtg atggattatc
    10561 aatggtgtga tttagcagat gtattacacg gtattgatgc aacgccgtgg gcgttcagtc
    10621 cgtggatggt gatgcaggcg acaaatcgcg aagccagaaa acgattatct gcatttaccc
    10681 agcttaaata agctttgcct caggattttt gaaatgacgg tctgcgcaaa aaaacacgtt
    10741 catctcactc gcgatgctgc ggagcagtta ctggctgata ttgatcgacg ccttgatcag
    10801 ttattgcccg tggagggaga acgggatgtt gtgggtgccg cgatgcgtga aggtgcgctg
    10861 gcaccgggaa aacgtattcg ccccatgttg ctgttgctga ccgcccgcga tctgggttgc
    10921 gctgtcagcc atgacggatt actggatttg gcctgtgcgg tggaaatggt ccacgcggct
    10981 tcgctgatcc ttgacgatat gccctgcatg gacgatgcga agctgcggcg cggacgccct
    11041 accattcatt ctcattacgg agagcatgtg gcaatactgg cggcggttgc cttgctgagt
    11101 aaagcctttg gcgtaattgc cgatgcagat ggcctcacgc cgctggcaaa aaatcgggcg
    11161 gtttctgaac tgtcaaacgc catcggcatg caaggattgg ttcagggtca gttcaaggat
    11221 ctgtctgaag gggataagcc gcgcagcgct gaagctattt tgatgacgaa tcactttaaa
    11281 accagcacgc tgttttgtgc ctcgatgcag atggcctcga ttgttgcgaa tgcctccagc
    11341 gaagcgcgtg attgcctgca tcgtttttca cttgatcttg gtcaggcatt tcaactgctg
    11401 gacgatttga ccgatggcat gaccgacacc ggtaaggata gcaatcagga cgccggtaaa
    11461 tcgacgctgg tcaatctgtt aggcccgagg gcggttgaag aacgtctgag acaacatctt
    11521 cagcttgcca gtgagcatct ctctgcggcc tgccaacacg ggcacgccac tcaacatttt
    11581 attcaggcct ggtttgacaa aaaactcgct gccgtcagtt aagcttgcaa gaattcaagc
    11641 ttggagtacg ttaattagtt gtctgtttta agccccgtgc tctccgccgg ggctttttta
    11701 ttaaaaacca ctcaagtgga agacaaggga cacgaagtga tccgtttaaa ctatcagtgt
    11761 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
    11821 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgcc agccgtgcgg
    11881 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg
    11941 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc
    12001 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg
    12061 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg
    12121 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg
    12181 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg
    12241 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg
    12301 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg
    12361 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg
    12421 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg
    12481 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt
    12541 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg
    12601 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg
    12661 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag
    12721 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca agg
//
