LOCUS       sequence-194305-       13401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13401)
  AUTHORS   Wilson JM, Hartley R, Maxwell DJ, Todd AJ, Lieberam I, Kaltschmidt 
            JA, Yoshida Y, Jessell TM, Brownstone RM
  TITLE     Conditional rhythmicity of ventral spinal interneurons defined by 
            expression of the Hb9 homeodomain protein.
  JOURNAL   J Neurosci. 2005 Jun 15. 25(24):5710-9.
  PUBMED    15958737
REFERENCE   2  (bases 1 to 13401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2005 Jun 15. 25(24):5710-9."
COMMENT     Sequence Label: pHB9-EGFP (Hb9 promoter) [TJ#96]
FEATURES             Location/Qualifiers
     source          1..13401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          8477..8706
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     8689..8708
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      8759..8768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8765..8767,8768..8770,8771..9484)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8765..8767,8768..8770,8771..9481)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8810..8831)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9071..9090)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9418..9439
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9518..9535)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9524..9748
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(9815..9833)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9854..9870)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9854..9870)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9867..9889)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9878..9894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9902..9908,9909..9926,9927..9932))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9947..9968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10085..10102)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10256..10844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10336..10355)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11015..11806,11807..11875))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11638..11657
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11876..11980)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12006..12461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12143..12164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12355..12374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12588..12610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12602..12619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12603..12619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12626..12645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12626..12644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        12679..12697
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gggcgggggc aattcacacc ctgtattctc accaggctaa agtttcttct atctggttcc
       61 ttctctccaa aaaatcagtc agaagggacc tcccttggct gagagtagaa gtgaggtggt
      121 cagcgtgctg cagacaagcc tgtcctccat ctgcctaagg ggtctgatat cctacaccaa
      181 gcccccccta ccccttctgg agacacatag gagataagca ttttcaaaac caccatggca
      241 ctctcctgtt ctcccctgca ccccttcctc ccagactcca tcctgcctga ggatgatttg
      301 gtccaacttg aataatagag tactctcttt ccacatcttt ccacatcgga aagagtagtc
      361 cgggatccag tccacctgca gccctgatta aaggaaggaa ggaagactag aggcgcagtt
      421 ctacctatta tacttatttt aaggttccca ggtcagagat gcctcatgga catttctccc
      481 taaggcaggt cctgaccagc cctccccctg aatggtgctg atctgtgact tgttgcttct
      541 attgaacaaa tacagctttg atttgtttcc agccagagtg gttagctgat gaattgacaa
      601 aaactaatca gctttattgg gaaacaggtt taagggcacg gacgtgtcaa taacgctcag
      661 cctgaccccc tcttccatta gctaggcagg ctgattagag taagaagacc ctctcgccga
      721 ttgcaccgaa attgctttca taaactcaca atattagcag tcgatgtgag cagctaattt
      781 agagaagaca tccaggcccg tcacatcgat catcgggttt atgtatcatt atctcattta
      841 cagaatgtcg cagtgtaata catgtttttg gcaataggct tggccgctcg ccggagccgc
      901 gcgctgttcc tttcggccac actgcagggg acttcctcaa caatctacga tgtctcttcg
      961 cgtgaacaag gaggcacttt gcacacatat gcacacttgc gcccaggctc tcagtgcgcg
     1021 ctcagtgcac acatgcacgt ggacacatgc tccgacatac tacctgagct gcctttcctt
     1081 ctcatgcggg gatgctggca ttgctctctc ctgcgagccc ctggtctgaa gcccaatgcc
     1141 ggacgctcac caaccttgga gtcaacctcg gagagctcaa ggttcggttc tgtcaccagc
     1201 atacagagat ggtgggtggc ggcgcaatca gccaccttct ctcaaggctt tggtcgccca
     1261 gttggcgggt gtccagaaat ccacaggcct gcgaaggtcc ttctccgccc tcccaggctc
     1321 cagcttgacc tgccctgccc tgggcgggcc tttctcgcaa cacttccagg ctcagccaga
     1381 tgcgcctggt cttagggagt gggccacctg ttcctttggg taattatttt aatgtaaggg
     1441 gtctacttca ggcctcctgc agtgacagac ggcagcggag ctgcaaagcg ctctcagcct
     1501 ttcagtggaa tttcccagat gggccaaggg gaggccaggg cgggtcacca ggggcccttt
     1561 aacgagttca ttagccaaat tatcttgcct gcagcaagga ccccagagac tgtagggaga
     1621 gtaggggaga aggtgggtct ttctccctcc ctgctagtct gcaagaacag ttgtgggaag
     1681 gaaagagaaa ggacatcctc cccaaagggg cctaggaaca ctaaaggcaa agatggcatc
     1741 tctgatgtga cttatgcgcc agaccagcga ggtggtggag tggtgggaag gaatcataat
     1801 gattaggaaa cacggagatt ctaacaacca gggcactgac ccatattgtg gaacaaacct
     1861 agtgcagaaa tcaaagtcag agcccctgga actttattct gtgcttctta agcaaagaac
     1921 ctggacatct tggagccttg gtctgtccgt ccatctgtag agtagaaggt aggtgtgttt
     1981 ccttccacaa tttgtgagat cactgcccta agaaaagcaa ctctgggaag agcttctggc
     2041 atgaccctgt tgggtccttc gggccccctt actcctgtgg taggaggctt tggcaggttg
     2101 aggtgagcct gatcctagat gcctgtgctg gaaaatgaac ccaactctca cctgtcgtcg
     2161 cgccccccac ccccacccca cagctgtgag gcgctgctct ggaggtagct cagcatcccc
     2221 gcccctcctc cttgtcctca ctctgcctcc tgtagtgtta gcttctgcta cctcagcaag
     2281 tagtgagtga atagaccaat tagccaggac ttcctgccat tatctgctgt agacttgggc
     2341 attagtacgc cctgattcac cataatttca agtcagaaca cactaaaaaa ttcactcacc
     2401 gaggcattaa aatgttccca gacaacgtct gtgtttgtgt aactgggttt cttcccttcg
     2461 tgtttgatgc acaccagcat catataggtc agtggtggag tccaggctag gcagacaatt
     2521 tttgtcgctt tttgtttgtt tttatatttt taaaacatga attgcattgt ttcccggtgg
     2581 ttgtaaactt tgtttctggg gcttcagtat tgccagtgta atcttcttct gcttgatcag
     2641 gggcaaacaa gtgacttaga aagttgtggg agtgccctaa gaacagtagc cagagccttt
     2701 acagagccag gctttgtgtc ctaaacgtac atcgccccag ctatgggaag ctgggttatc
     2761 accttcaaag cagacccttt ctgtggatga cttaccaagc cctgcaggtg ggtagtggag
     2821 gtggggagag ggctgcacag cctgcttgga gcttgcttct gttctttgtc ctgcccatga
     2881 tatgaagctc ctatacaatg aatttgcagt aagatcttgg agcaaaagta caggtttcct
     2941 gagaagtctt tcgtttcttc tgagggtgct gttttactta cacacacaca cacacacaca
     3001 cacacacaca cacacacaca cacaccctag ctgattaaaa gaactgaggt gacctctctt
     3061 agccttgttt ctctttatga ttcataccta tggcctctag gattgtaaga gaaattgaaa
     3121 atgtctttgg cttcctgggt cagaaagcat ccagcgcagg gctgagctca gtctcttctc
     3181 tatgttctaa gtacacagca aaacaaacaa aaacccgaaa ccttgagtaa aattttcaaa
     3241 tatagaatat atttttgtgc tggagttttt caaatttata tcttactgtc tttctccctg
     3301 tacccctaca acccaggttt gggctggact tatttattta tttacaagga ggaatggggc
     3361 cagtggagaa gggcagccag tgaagatggc ctgagggtgg accaggcagt agggagggca
     3421 gagtaagctt tctaaaaagg gaggaaggaa atccaagtgt gctgggagag gtagagaagg
     3481 ggggggggct ggcaaagaaa aggactggct tgtagtgtcc ttggcaagag cagttcacct
     3541 caggttggaa cactgggtct tcaggtcacc tgtcttctga gccacctttg caaaccaccc
     3601 ctttaaagct gaactgggga tggaggtctg agaaaagggt ggtagatcaa aggatggtaa
     3661 taaaggagct gatggtactt ttgggctctg aaggggctgt aatgagtgtg tgtgtgtggg
     3721 gggggactca tcagacatcc cttctcctga tctcacctcc ctgtgacccc aggctcccca
     3781 ttggattctg tgtgtcctca ggctggggtg ggtggacggg ttatgtcccc agagacattc
     3841 caagcactga tttcagtctg acttgaagga agttggtcta tgaccttcca ggtccagcat
     3901 tcacacttcc tgcttgcttt ctgtcctcct gagtacttat tcagcctctg aaaaatacca
     3961 ccatcaccac ccctgtgggc ttgtgtcctg gacctcactg gcctcactac atcctccagg
     4021 ctcattttcc tgaagggaag ccttaaaatg tttttttttt taaagattta tttatttatt
     4081 atatgtaagt acattgtagc tgtcttcaga cacaccagaa gagggagtca gatctcgtta
     4141 cagatggttg tgagccacca tgtggttgct gggatttgaa ctctggacct tcggaagagc
     4201 agtagggtgc tctaacccac tgagccatct caccagcccc gcctcaaaat gttgatgtga
     4261 gttaactctc atggagttca aaagggctga gaagtcagtc ttcatgaacc tttctccatg
     4321 aggctgctgt gggcaggggg ttgcttgttt taagttttgg aaacagccag acaaatggca
     4381 ggtgtgtgga gggagatgca gggaatgctg ccttccatcc agtctcctag ccatacctcc
     4441 aggctcttgg cctttttgtt aatggctatt atatattgta cctgctgcat tccacagaaa
     4501 gggatgtgtt tgtaggtgat cagttataga atcaatgctg acctgacctg tgaatatggg
     4561 tgcatgtagg ttttgttctg agttcttgta taatgtcagg ggtaaaactt gtagattata
     4621 acatctttgt aagttttctt tttatgtttg cattgtgtgt atgtttgtgt ctatttatgg
     4681 gaatgcacgt acccaaagag gccataagag ggaatccgat ttcctgtagc tggagttgga
     4741 ggtggttgtg agtgtgagtg gtgggaaccc aacttgggtc ctcctcaaga gctgcactca
     4801 ctcttaagca ctgaactgtg gaaccactat actccccacc ccaacatctt atttccttgt
     4861 ggttatttgt ataaatcaac acacctttct cagtaatgag aggaggctca atcacaagtt
     4921 atattcagta acttaatgca agacccaaaa gagggatcct ggctagacgc agagaaaaaa
     4981 atgactggga gccagatgat taaatgtaca ccgggcctta aattccagcc tcttttgggt
     5041 aggagaggtc ttcctctctg ccctccaaga gcctcatttg gcccctgggg cttctggaga
     5101 agggagctac cttgaggcac aacaatggga atgctgtgct cacttcggca gcacctatac
     5161 taaaactgaa ccgatacaga gaagattagc atggcccctg cgcaaggatg acatgcaaat
     5221 ttgtgaagca ttccttattt ttttttgcca ggaccaggaa tgggaacagg tgggttgggg
     5281 agcagaggga ggggagaggg gataggggat ttggggaggg gaaactagga aaggggataa
     5341 catttgaaat gtaaataaag aaaatatcta ataaaaaaaa aaaaaagaat aggcatgctt
     5401 ctgggaccag acctttgatc tctcaagggt accgtagtaa acccccagct cagtcccagg
     5461 ctgccccaga gccagctggg ctgggtcaat agacgctggg aacaggaaga gacgcagcat
     5521 gctcactcca ctcgtaaaac acacaaagat cccagttcca gacgactccc agtctctctc
     5581 ccaactccca cctctcagga tacaagttcc cgctcaaacc gaagcagaat atatgatcat
     5641 attcaagccc tttgacttca gagtcttttt ggcccaaccc ccactttgtc aaacaaagaa
     5701 atatatccat caagtctgat atgcaaattt ccccttcgct gaaggggctc ttgatacaac
     5761 ctcactcagc ccacaggtgg tggggactgg tgtgggttag cctggtggat ggtcaaacac
     5821 cagacgaagt gggaagggtt tgaaagggat ttagaagctt ttggatcaca tcccctacac
     5881 aacaaacagg aaatgaattc ctcttctcgg atgactttac taaggaaacc tgtgcctaaa
     5941 ctcagtagac ctaatactgt atcatcacca aaaacaaccc tggttgtgta gtttagagat
     6001 gattcgggtc catgaggtcc accccaggaa aatctcatct gcagccaccc tttttttcct
     6061 ccttcttctc ctttgtttgt tttgttgttg tttttttcaa gacagggtat ctctgtgtag
     6121 catttttggt tgctgtcctg gaactggctc tgtagactag gctggcctag aactcacaga
     6181 ccccacctgc ctctgcctcc tgagtgctgg gattagagac ttgcctcacc atggcttaaa
     6241 cacaagtttc tgtaaatgca atggggtcaa gactgaatcc tggggatggg ggtagggcat
     6301 cccagcatcc ttacttgcct taattgggct tccaagatag gagaactggc agggaaccac
     6361 tcatggaaca caagtgttcc tttaaccctc taaaaggcag agctgcattt tcatcacccc
     6421 aaagagacag cacctaaaga caagagctat gtgcttgtac tattttcaga ctagctggag
     6481 gcctttgcaa agctgtgcac aacttactcc aagatgttcc ccgctgggtt ccagattttc
     6541 cagccaggac caccctgtca taataaaaac tccactcagc cgagcatggt ggcgcacacc
     6601 tttaatccca gcacttggga ggcagaggca ggaggatttc tgagttcgag gccagcctgg
     6661 tctacagagt gagttccaag acagccaggg ctagacagag aaactctgtc tccaaaatca
     6721 aaaacaaaaa caaaacaaaa acaacaaaaa caaaaaacct actcactcat cccaacagaa
     6781 tcactgcgtt aaatctctcc taaacaaaac ttgttgacag ggaaaatata gtccttactt
     6841 atatttatcg ttacccccca cccccaatcc ctcttgaaac agctttgcct gctactttta
     6901 aattcaccca acaaggattg tcctagcctc ctggccttcc ctgggccatt tccgatcttt
     6961 tattaacctc tcacctggtt gggaagccgc gatccagaga cctggaactc cctcccactt
     7021 tcccctcgtg tttggggaag atacccgaga atggagctag cgagaaagct tgatttctca
     7081 aactacttat tgattcgtta cattaaaaaa aaaaagcccc cggaagcgtg tttacagtaa
     7141 atatttgata tgtgcgcagg ctgaggtctg gattcacact ttaatacggt ctctggaagc
     7201 cctttcttga ttttcctgaa ctcggccggc aggaggaggc gagagcctga tagtggctcc
     7261 cttagcctgc gaactggctc agtccgaggg agcagtcctc ggccaattcc cgcaaagcct
     7321 ttttcctaca cccccacaag gcaaggctct aatagaactt ggttctcacc taggtccggc
     7381 tcagtggcac ctgtgcaaac gaccttcaag tcaatgtgca tctctgagtt ggaaaactgc
     7441 tctctctcca ctctgactga agatttgtag gcttcaacct ctaataccgt atttatacac
     7501 accgaaaccc tacaaacaaa catttgcggc gcatgatact tttgggggag gggcagataa
     7561 gaaggttcga cctgctgagg gttaatgaca gggacgcaaa ccatcttgaa gcaatcaaca
     7621 ctcaggaacg cgagagattt tgggaaaccg aacagtcttc agtgtactaa gctcagggtc
     7681 ccgttttggg tacctagtgg acttcagggg tgaggaccca cctagttcct cagtgcagaa
     7741 cattccacaa ctccctcaag ggagcgcgag tagctggagg aagataaggc gtccgcctct
     7801 ggaggaacaa ggtgtcaaag ggactcgggg ggcactcact gggacccagg gttactggcg
     7861 ctcaccactc tcccagcagc aaggaagcag taagcttgtg ttcagcctcc aaactctcca
     7921 acctgcctcc tccggccacc tacgctgttc accaccggct gcttaggacc tgggggcgca
     7981 ggggcggggc gtcaggctaa ttggaccgtg acaggtgagc ccctcggcca atcgccgggc
     8041 agacagccct cctcgggccc ccgctgagcg cgccaatccc agaccgccct gagcttcaag
     8101 tgtgcggagg ggcgcgggtc cccaccaagg caccagtggc ctcgatgcgg tcgcttgagc
     8161 tcttgagagc agagagccgg tctccccaac acaaactcgc aggagcgctc cggtggaagt
     8221 tcataccagt gagtggagcg cggtccccac gaggaccgca aagaggaggc gcctggacga
     8281 tggtcgctct cagctgagtt tccggctgcg actttattgg caaaaaatcg cagtaacaat
     8341 accggcccca aggctggcca ccgccacgct cagtctccgt aagcccgcac acgactgcat
     8401 cgcccaccct tggaccctga ttgtcagctc caatcgagcc ggcgcgcctc gctgtctgcg
     8461 agggccagct gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga
     8521 ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg
     8581 agggtggccg cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt
     8641 ggcaggcttg agatctggcc atacacttga gtgacaatga catccacttt gcctttctct
     8701 ccacaggtgt ccactcccag gtccaactgc agtccaagcg gatccccggg taccggtcgc
     8761 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     8821 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     8881 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     8941 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     9001 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     9061 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     9121 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     9181 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     9241 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     9301 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     9361 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     9421 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     9481 gtaaagcggc ccgctagccc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     9541 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     9601 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     9661 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     9721 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     9781 agtctagttt aattaagagc tccagctttt gttcccttta gtgagggtta atttcgagct
     9841 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     9901 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     9961 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    10021 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10081 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10141 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10201 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10261 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10321 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10381 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    10441 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    10501 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    10561 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10621 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10681 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    10741 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    10801 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    10861 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    10921 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    10981 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11041 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11101 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11161 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11221 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11281 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    11341 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    11401 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    11461 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    11521 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    11581 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    11641 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    11701 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    11761 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    11821 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    11881 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    11941 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12001 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
    12061 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
    12121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
    12181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
    12241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
    12301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
    12361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
    12421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
    12481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    12541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    12601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccct
    12661 cgaggcggcc gcgagctcaa ttaaccctca ctaaagggag tcgactcgat cctgtctccc
    12721 tggccaggag tctggggtcc gcagatatat tacaacaaaa ttactaattg gaaaatgaaa
    12781 ttgtcaataa agcacacccc acgcgtgtac tatatcttta aatattatga tgtcttattg
    12841 gtcccatttc cttacaataa tttattgcaa taaaacagac actgcaggtc cccttaaatt
    12901 gtcttttcat aatgaataaa tttaagcagg ctaattaata tataaactag ctcaatttgt
    12961 caagttgatt tgtattttag ttaattgtga aagtaattac cacatggtca aattaacagc
    13021 tttctggaaa tgaccaagcc tgaggtttta tttccttcct gggtgaagaa aattcatttt
    13081 tccaagctct tgatgtgatg aataaaagtc ataaatctgg gtgattggtg caggcagagt
    13141 ctaaatggct tcatatttca ttttaggttt aatagaaata ttcatgctct gttttaatga
    13201 aattaaattg aagggggatg gggctctgga ggcggccctg gctgggggct taaagggaca
    13261 gtgcactttt cctttcttca tgcaagggcc agggctagag gtccctggac tcagtcaaga
    13321 agtggagaga gctgagggag gcctgggagg cccttattct gtacccagtg tagcacacct
    13381 gcagccctga ttgggggggg g
//
