LOCUS       sequence-194313-       15471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a 
            CTAG addition in CA at nt 1508..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   McCauley SM, Kim K, Nowosielska A, Dauphin A, Yurkovetskiy L, Diehl 
            WE, Luban J
  TITLE     Intron-containing RNA from the HIV-1 provirus activates type I 
            interferon and inflammatory cytokines.
  JOURNAL   Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2.
  PUBMED    30546110
REFERENCE   2  (bases 1 to 15471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-13"; volume: "9(1):5305. doi"; pages: " 
            10.1038/s41467-018-07753-2"
COMMENT     Sequence Label: pNL4-3 envFS eGFP gag2xFS
FEATURES             Location/Qualifiers
     source          1..15471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             2091..5102
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4787..4904
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7767..8000
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8185..8229
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8523..8549
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(8816..8818,8819..9532)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8816..8818,8819..9529)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8858..8879)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9119..9138)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9466..9487
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             9722..10355
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11995..12012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12166..12754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12246..12265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12925..13716,13717..13785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13548..13567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13786..13890)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13958..13976
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14014..14036)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14136..14155
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga tcgaaatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aacaatataa actaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct tttagagaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacaatagca gtcctctatt gtgtgcatca aaggatagat gtaaaagaca
     1081 ccaaggaagc cttagataag atagaggaag agcaaaacaa aagtaagaaa aaggcacagc
     1141 aagcagcagc tgacacagga aacaacagcc aggtcagcca aaattaccct atagtgcaga
     1201 acctccaggg gcaaatggta catcaggcca tatcacctag aactttaaat gcatgggtaa
     1261 aagtagtaga agagaaggct ttcagcccag aagtaatacc catgttttca gcattatcag
     1321 aaggagccac cccacaagat ttaaatacca tgctaaacac agtgggggga catcaagcag
     1381 ccatgcaaat gttaaaagag accatcaatg aggaagctgc agaatgggat agattgcatc
     1441 cagtgcatgc agggcctatt gcaccaggcc agatgagaga accaagggga agtgacatag
     1501 caggaactac tagctagtac ccttcaggaa caaataggat ggatgacaca taatccacct
     1561 atcccagtag gagaaatcta taaaagatgg ataatcctgg gattaaataa aatagtaaga
     1621 atgtatagcc ctaccagcat tctggacata agacaaggac caaaggaacc ctttagagac
     1681 tatgtagacc gattctataa aactctaaga gccgagcaag cttcacaaga ggtaaaaaat
     1741 tggatgacag aaaccttgtt ggtccaaaat gcgaacccag attgtaagac tattttaaaa
     1801 gcattgggac caggagcgac actagaagaa atgatgacag catgtcaggg agtgggggga
     1861 cccggccata aagcaagagt tttggctgaa gcaatgagcc aagtaacaaa tccagctacc
     1921 ataatgatac agaaaggcaa ttttaggaac caaagaaaga ctgttaagtg tttcaattgt
     1981 ggcaaagaag ggcacatagc caaaaattgc agggccccta ggaaaaaggg ctgttggaaa
     2041 tgtggaaagg aaggacacca aatgaaagat tgtactgaga gacaggctaa ttttttaggg
     2101 aagatctggc cttcccacaa gggaaggcca gggaattttc ttcagagcag accagagcca
     2161 acagccccac cagaagagag cttcaggttt ggggaagaga caacaactcc ctctcagaag
     2221 caggagccga tagacaagga actgtatcct ttagcttccc tcagatcact ctttggcagc
     2281 gacccctcgt cacaataaag ataggggggc aattaaagga agctctatta gatacaggag
     2341 cagatgatac agtattagaa gaaatgaatt tgccaggaag atggaaacca aaaatgatag
     2401 ggggaattgg aggttttatc aaagtaagac agtatgatca gatactcata gaaatctgcg
     2461 gacataaagc tataggtaca gtattagtag gacctacacc tgtcaacata attggaagaa
     2521 atctgttgac tcagattggc tgcactttaa attttcccat tagtcctatt gagactgtac
     2581 cagtaaaatt aaagccagga atggatggcc caaaagttaa acaatggcca ttgacagaag
     2641 aaaaaataaa agcattagta gaaatttgta cagaaatgga aaaggaagga aaaatttcaa
     2701 aaattgggcc tgaaaatcca tacaatactc cagtatttgc cataaagaaa aaagacagta
     2761 ctaaatggag aaaattagta gatttcagag aacttaataa gagaactcaa gatttctggg
     2821 aagttcaatt aggaatacca catcctgcag ggttaaaaca gaaaaaatca gtaacagtac
     2881 tggatgtggg cgatgcatat ttttcagttc ccttagataa agacttcagg aagtatactg
     2941 catttaccat acctagtata aacaatgaga caccagggat tagatatcag tacaatgtgc
     3001 ttccacaggg atggaaagga tcaccagcaa tattccagtg tagcatgaca aaaatcttag
     3061 agccttttag aaaacaaaat ccagacatag tcatctatca atacatggat gatttgtatg
     3121 taggatctga cttagaaata gggcagcata gaacaaaaat agaggaactg agacaacatc
     3181 tgttgaggtg gggatttacc acaccagaca aaaaacatca gaaagaacct ccattccttt
     3241 ggatgggtta tgaactccat cctgataaat ggacagtaca gcctatagtg ctgccagaaa
     3301 aggacagctg gactgtcaat gacatacaga aattagtggg aaaattgaat tgggcaagtc
     3361 agatttatgc agggattaaa gtaaggcaat tatgtaaact tcttagggga accaaagcac
     3421 taacagaagt agtaccacta acagaagaag cagagctaga actggcagaa aacagggaga
     3481 ttctaaaaga accggtacat ggagtgtatt atgacccatc aaaagactta atagcagaaa
     3541 tacagaagca ggggcaaggc caatggacat atcaaattta tcaagagcca tttaaaaatc
     3601 tgaaaacagg aaagtatgca agaatgaagg gtgcccacac taatgatgtg aaacaattaa
     3661 cagaggcagt acaaaaaata gccacagaaa gcatagtaat atggggaaag actcctaaat
     3721 ttaaattacc catacaaaag gaaacatggg aagcatggtg gacagagtat tggcaagcca
     3781 cctggattcc tgagtgggag tttgtcaata cccctccctt agtgaagtta tggtaccagt
     3841 tagagaaaga acccataata ggagcagaaa ctttctatgt agatggggca gccaataggg
     3901 aaactaaatt aggaaaagca ggatatgtaa ctgacagagg aagacaaaaa gttgtccccc
     3961 taacggacac aacaaatcag aagactgagt tacaagcaat tcatctagct ttgcaggatt
     4021 cgggattaga agtaaacata gtgacagact cacaatatgc attgggaatc attcaagcac
     4081 aaccagataa gagtgaatca gagttagtca gtcaaataat agagcagtta ataaaaaagg
     4141 aaaaagtcta cctggcatgg gtaccagcac acaaaggaat tggaggaaat gaacaagtag
     4201 ataaattggt cagtgctgga atcaggaaag tactattttt agatggaata gataaggccc
     4261 aagaagaaca tgagaaatat cacagtaatt ggagagcaat ggctagtgat tttaacctac
     4321 cacctgtagt agcaaaagaa atagtagcca gctgtgataa atgtcagcta aaaggggaag
     4381 ccatgcatgg acaagtagac tgtagcccag gaatatggca gctagattgt acacatttag
     4441 aaggaaaagt tatcttggta gcagttcatg tagccagtgg atatatagaa gcagaagtaa
     4501 ttccagcaga gacagggcaa gaaacagcat acttcctctt aaaattagca ggaagatggc
     4561 cagtaaaaac agtacataca gacaatggca gcaatttcac cagtactaca gttaaggccg
     4621 cctgttggtg ggcggggatc aagcaggaat ttggcattcc ctacaatccc caaagtcaag
     4681 gagtaataga atctatgaat aaagaattaa agaaaattat aggacaggta agagatcagg
     4741 ctgaacatct taagacagca gtacaaatgg cagtattcat ccacaatttt aaaagaaaag
     4801 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     4861 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     4921 acagcagaga tccagtttgg aaaggaccag caaagctcct ctggaaaggt gaaggggcag
     4981 tagtaataca agataatagt gacataaaag tagtgccaag aagaaaagca aagatcatca
     5041 gggattatgg aaaacagatg gcaggtgatg attgtgtggc aagtagacag gatgaggatt
     5101 aacacatgga aaagattagt aaaacaccat atgtatattt caaggaaagc taaggactgg
     5161 ttttatagac atcactatga aagtactaat ccaaaaataa gttcagaagt acacatccca
     5221 ctaggggatg ctaaattagt aataacaaca tattggggtc tgcatacagg agaaagagac
     5281 tggcatttgg gtcagggagt ctccatagaa tggaggaaaa agagatatag cacacaagta
     5341 gaccctgacc tagcagacca actaattcat ctgcactatt ttgattgttt ttcagaatct
     5401 gctataagaa ataccatatt aggacgtata gttagtccta ggtgtgaata tcaagcagga
     5461 cataacaagg taggatctct acagtacttg gcactagcag cattaataaa accaaaacag
     5521 ataaagccac ctttgcctag tgttaggaaa ctgacagagg acagatggaa caagccccag
     5581 aagaccaagg gccacagagg gagccataca atgaatggac actagagctt ttagaggaac
     5641 ttaagagtga agctgttaga cattttccta ggatatggct ccataactta ggacaacata
     5701 tctatgaaac ttacggggat acttgggcag gagtggaagc cataataaga attctgcaac
     5761 aactgctgtt tatccatttc agaattgggt gtcgacatag cagaataggc gttactcgac
     5821 agaggagagc aagaaatgga gccagtagat cctagactag agccctggaa gcatccagga
     5881 agtcagccta aaactgcttg taccaattgc tattgtaaaa agtgttgctt tcattgccaa
     5941 gtttgtttca tgacaaaagc cttaggcatc tcctatggca ggaagaagcg gagacagcga
     6001 cgaagagctc atcagaacag tcagactcat caagcttctc tatcaaagca gtaagtagta
     6061 catgtaatgc aacctataat agtagcaata gtagcattag tagtagcaat aataatagca
     6121 atagttgtgt ggtccatagt aatcatagaa tataggaaaa tattaagaca aagaaaaata
     6181 gacaggttaa ttgatagact aatagaaaga gcagaagaca gtggcaatga gagtgaagga
     6241 gaagtatcag cacttgtgga gatgggggtg gaaatggggc accatgctcc ttgggatatt
     6301 gatgatctgt agtgctacag aaaaattgtg ggtcacagtc tattatgggg tacctgtgtg
     6361 gaaggaagca accaccactc tattttgtgc atcagatgct aaagcatata tgatacagag
     6421 gtacataatg tttgggccac acatgcctgt gtacccacag accccaaccc acaagaagta
     6481 gtattggtaa atgtgacaga aaattttaac atgtggaaaa atgacatggt agaacagatg
     6541 catgaggata taatcagttt atgggatcaa agcctaaagc catgtgtaaa attaacccca
     6601 ctctgtgtta gtttaaagtg cactgatttg aagaatgata ctaataccaa tagtagtagc
     6661 gggagaatga taatggagaa aggagagata aaaaactgct ctttcaatat cagcacaagc
     6721 ataagagata aggtgcagaa agaatatgca ttcttttata aacttgatat agtaccaata
     6781 gataatacca gctataggtt gataagttgt aacacctcag tcattacaca ggcctgtcca
     6841 aaggtatcct ttgagccaat tcccatacat tattgtgccc cggctggttt tgcgattcta
     6901 aaatgtaata ataagacgtt caatggaaca ggaccatgta caaatgtcag cacagtacaa
     6961 tgtacacatg gaatcaggcc agtagtatca actcaactgc tgttaaatgg cagtctagca
     7021 gaagaagatg tagtaattag atctgccaat ttcacagaca atgctaaaac cataatagta
     7081 cagctgaaca catctgtaga aattaattgt acaagaccca acaacaatac aagaaaaagt
     7141 atccgtatcc agaggggacc agggagagca tttgttacaa taggaaaaat aggaaatatg
     7201 agacaagcac attgtaacat tagtagagca aaatggaatg ccactttaaa acagatagct
     7261 agcaaattaa gagaacaatt tggaaataat aaaacaataa tctttaagca atcctcagga
     7321 ggggacccag aaattgtaac gcacagtttt aattgtggag gggaattttt ctactgtaat
     7381 tcaacacaac tgtttaatag tacttggttt aatagtactt ggagtactga agggtcaaat
     7441 aacactgaag gaagtgacac aatcacactc ccatgcagaa taaaacaatt tataaacatg
     7501 tggcaggaag taggaaaagc aatgtatgcc cctcccatca gtggacaaat tagatgttca
     7561 tcaaatatta ctgggctgct attaacaaga gatggtggta ataacaacaa tgggtccgag
     7621 atcttcagac ctggaggagg cgatatgagg gacaattgga gaagtgaatt atataaatat
     7681 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     7741 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     7801 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     7861 gatatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     7921 caactcacag tctggggcat caaacagctc caggcaagaa tcctggctgt ggaaagatac
     7981 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     8041 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaataacatg
     8101 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     8161 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     8221 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     8281 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     8341 agagttaggc agggatattc accattatcg tttcagaccc acctcccaat cccgagggga
     8401 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     8461 cgattagtga acggatcctt agcacttatc tgggacgatc tgcggagcct gtgcctcttc
     8521 agctaccacc gcttgagaga cttactcttg attgtaacga ggattgtgga acttctggga
     8581 cgcagggggt gggaagccct caaatattgg tggaatctcc tacaatattg gagtcaggag
     8641 ctaaagaata gtgctgttag cttgctcaat gccacagcca tagcagtagc tgaggggaca
     8701 gatagggtta tagaagtagt acaaggagct tgtagagcta ttcgccacat acctagaaga
     8761 ataagacagg gcttggaaag gattttgcta taagatgggt ggcgcggccg ctctggtgag
     8821 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     8881 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     8941 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     9001 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     9061 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     9121 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     9181 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     9241 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     9301 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     9361 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     9421 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     9481 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aactcgagac
     9541 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaacaatgc tgcttgtgcc
     9601 tggctagaag cacaagagga ggaagaggtg ggttttccag tcacacctca ggtaccttta
     9661 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     9721 ctggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
     9781 acacaaggct acttccctga ttggcagaac tacacaccag ggccaggggt cagatatcca
     9841 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     9901 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatggaat ggatgaccct
     9961 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
    10021 gagctgcatc cggagtactt caagaactgc tgacatcgag cttgctacaa gggactttcc
    10081 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
    10141 tgctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
    10201 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10261 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10321 cagacccttt tagtcagtgt ggaaaatctc tagcacccag gaggtagagg ttgcagtgag
    10381 ccaagatcgc gccactgcat tccagcctgg gcaagaaaac aagactgtct aaaataataa
    10441 taataagtta agggtattaa atatatttat acatggaggt cataaaaata tatatatttg
    10501 ggctgggcgc agtggctcac acctgcgccc ggccctttgg gaggccgagg caggtggatc
    10561 acctgagttt gggagttcca gaccagcctg accaacatgg agaaacccct tctctgtgta
    10621 tttttagtag attttatttt atgtgtattt tattcacagg tatttctgga aaactgaaac
    10681 tgtttttcct ctactctgat accacaagaa tcatcagcac agaggaagac ttctgtgatc
    10741 aaatgtggtg ggagagggag gttttcacca gcacatgagc agtcagttct gccgcagact
    10801 cggcgggtgt ccttcggttc agttccaaca ccgcctgcct ggagagaggt cagaccacag
    10861 ggtgagggct cagtccccaa gacataaaca cccaagacat aaacacccaa caggtccacc
    10921 ccgcctgctg cccaggcaga gccgattcac caagacggga attaggatag agaaagagta
    10981 agtcacacag agccggctgt gcgggagaac ggagttctat tatgactcaa atcagtctcc
    11041 ccaagcattc ggggatcaga gtttttaagg ataacttagt gtgtaggggg ccagtgagtt
    11101 ggagatgaaa gcgtagggag tcgaaggtgt ccttttgcgc cgagtcagtt cctgggtggg
    11161 ggccacaaga tcggatgagc cagtttatca atccgggggt gccagctgat ccatggagtg
    11221 cagggtctgc aaaatatctc aagcactgat tgatcttagg ttttacaata gtgatgttac
    11281 cccaggaaca atttggggaa ggtcagaatc ttgtagcctg tagctgcatg actcctaaac
    11341 cataatttct tttttgtttt ttttttttta tttttgagac agggtctcac tctgtcacct
    11401 aggctggagt gcagtggtgc aatcacagct cactgcagcc tcaacgtcgt aagctcaagc
    11461 gatcctccca cctcagcctg cctggtagct gagactacaa gcgacgcccc agttaatttt
    11521 tgtatttttg gtagaggcag cgttttgccg tgtggccctg gctggtctcg aactcctggg
    11581 ctcaagtgat ccagcctcag cctcccaaag tgctgggaca accggggcca gtcactgcac
    11641 ctggccctaa accataattt ctaatctttt ggctaatttg ttagtcctac aaaggcagtc
    11701 tagtccccag gcaaaaaggg ggtttgtttc gggaaagggc tgttactgtc tttgtttcaa
    11761 actataaact aagttcctcc taaacttagt tcggcctaca cccaggaatg aacaaggaga
    11821 gcttggaggt tagaagcacg atggaattgg ttaggtcaga tctctttcac tgtctgagtt
    11881 ataattttgc aatggtggtt caaagactgc ccgcttctga caccagtcgc tgcattaatg
    11941 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    12001 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    12061 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    12121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    12181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    12241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    12301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    12361 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    12421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    12481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    12541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    12601 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    12661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    12721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    12781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    12841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    12901 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    12961 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    13021 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    13081 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    13141 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    13201 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    13261 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    13321 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    13381 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    13441 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    13501 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    13561 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    13621 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    13681 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    13741 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    13801 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    13861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    13921 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    13981 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    14041 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    14101 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    14161 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    14221 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    14281 gctattacgc caggggaggc agagattgca gtaagctgag atcgcagcac tgcactccag
    14341 cctgggcgac agagtaagac tctgtctcaa aaataaaata aataaatcaa tcagatattc
    14401 caatcttttc ctttatttat ttatttattt tctattttgg aaacacagtc cttccttatt
    14461 ccagaattac acatatattc tatttttctt tatatgctcc agtttttttt agaccttcac
    14521 ctgaaatgtg tgtatacaaa atctaggcca gtccagcaga gcctaaaggt aaaaaataaa
    14581 ataataaaaa ataaataaaa tctagctcac tccttcacat caaaatggag atacagctgt
    14641 tagcattaaa taccaaataa cccatcttgt cctcaataat tttaagcgcc tctctccacc
    14701 acatctaact cctgtcaaag gcatgtgccc cttccgggcg ctctgctgtg ctgccaacca
    14761 actggcatgt ggactctgca gggtccctaa ctgccaagcc ccacagtgtg ccctgaggct
    14821 gccccttcct tctagcggct gcccccactc ggctttgctt tccctagttt cagttacttg
    14881 cgttcagcca aggtctgaaa ctaggtgcgc acagagcggt aagactgcga gagaaagaga
    14941 ccagctttac agggggttta tcacagtgca ccctgacagt cgtcagcctc acagggggtt
    15001 tatcacattg caccctgaca gtcgtcagcc tcacaggggg tttatcacag tgcaccctta
    15061 caatcattcc atttgattca caattttttt agtctctact gtgcctaact tgtaagttaa
    15121 atttgatcag aggtgtgttc ccagagggga aaacagtata tacagggttc agtactatcg
    15181 catttcaggc ctccacctgg gtcttggaat gtgtcccccg aggggtgatg actacctcag
    15241 ttggatctcc acaggtcaca gtgacacaag ataaccaaga cacctcccaa ggctaccaca
    15301 atgggccgcc ctccacgtgc acatggccgg aggaactgcc atgtcggagg tgcaagcaca
    15361 cctgcgcatc agagtccttg gtgtggaggg agggaccagc gcagcttcca gccatccacc
    15421 tgatgaacag aacctaggga aagccccagt tctacttaca ccaggaaagg c
//
