LOCUS       sequence-194323-        2666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  chimeric spike resembling V4 region of prokaryotic 16S rRNA to be 
            used in metagenomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2666)
  TITLE     metagenomic_spikes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSPIKE-P
FEATURES             Location/Qualifiers
     source          1..2666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     225..247
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     239..256
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     240..256
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     267..286
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        267..285
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(756..773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(866..1454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(946..965)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1625..2416,2417..2485))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2248..2267
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2486..2590)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
       61 agaccgagat agggttgagt ggccgctaca gggcgctccc attcgccatt caggctgcgc
      121 aactgttggg aagggcgttt cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      181 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      241 taaaacgacg gccagtgagc gcgacgtaat acgactcact atagggcgaa ttggcggaag
      301 gccgtcaagg ccacgtgtct tgtccagagc tcgtgccagc agccgcggta atcccttgtc
      361 tccctacctc tggaggagaa aagtgttgac atgggcgctc ccggcgcaag ggccaaagga
      421 gtctccgatt tcttattccc gaatgacatg cgtctccctg cgggtaaatc accgaccgcg
      481 attcatagaa gcctggggga acaggtaggt ctaactagct taagagagta aatcctggga
      541 tcattcagta gtaaccacaa acttacgctg gggcttcttc ggcggatctt ttacgagact
      601 gattattaga aaccctagta gtccggtacc tggagcacaa gactggcctc atgggccttc
      661 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat
      721 agctgtttcc ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg
      781 gtcgttcggg taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc
      841 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      901 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      961 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1021 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1081 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1141 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1201 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1261 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1321 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1381 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1441 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1501 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1561 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1621 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1681 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1741 gccccagtgc tgcaatgata ccgcgagaac cacgctcacc ggctccagat ttatcagcaa
     1801 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1861 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1921 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1981 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2041 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2101 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2161 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2221 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2281 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2341 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2401 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2461 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2521 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2581 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     2641 aaaattcgcg ttaaattttt gttaaa
//
