LOCUS       sequence-194324-        2592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  chimeric spike resembling a fragment of eukaryotic 18S rRNA to be 
            used in metagenomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2592)
  TITLE     metagenomic_spikes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSPIKE-E
FEATURES             Location/Qualifiers
     source          1..2592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     229..251
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     243..260
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     244..260
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     271..290
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        271..289
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(686..703)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(796..1384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(876..895)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1555..2346,2347..2415))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2178..2197
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2416..2520)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
       61 gaatagaccg agatagggtt gagtggccgc tacagggcgc tcccattcgc cattcaggct
      121 gcgcaactgt tgggaagggc gtttcggtgc gggcctcttc gctattacgc cagctggcga
      181 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      241 gttgtaaaac gacggccagt gagcgcgacg taatacgact cactataggg cgaattggcg
      301 gaaggccgtc aaggccacgt gtcttgtcca ggcgcgccat ctgtgatgcc cttagatgtt
      361 ctgggtccct tgtctcccga gaaagtcata gtacctcggg taccaactta ctcaggttat
      421 tgcttgaagc tgtaccattt taggggggga gcgctgaagg tctcttcttc tcatgactga
      481 actcgcgagg gtcgtgaagt cggttccttc aatggttacg agactgattc cctgcccttt
      541 gtacacaccg cattaattaa tggagcacaa gactggcctc atgggccttc cgctcactgc
      601 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat agctgtttcc
      661 ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggg
      721 taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1201 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     1561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1681 tgcaatgata ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     2041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     2101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     2161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     2221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     2281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     2341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     2401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     2521 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     2581 ttaaattttt gt
//
