LOCUS       sequence-194334-        3044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains model Rp51a with a TACTAAC->GTTAGTG BP mutataion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Larson JD, Hoskins AA
  TITLE     Dynamics and consequences of spliceosome E complex formation.
  JOURNAL   Elife. 2017 Aug 22;6. pii: 27592. doi: 10.7554/eLife.27592.
  PUBMED    28829039
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-22"; volume: "6. pii: 27592. doi"; pages: " 
            10.7554/eLife.27592"
COMMENT     Sequence Label: Rp51a_No_BP-pUC18
FEATURES             Location/Qualifiers
     source          1..3044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(27..49)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     149..168
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     362..384
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     376..393
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     377..393
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     418..437
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        418..436
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(821..837)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(821..837)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(834..856)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    845..861
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(869..875,876..893,894..899))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    914..935
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1052..1069)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1223..1811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1303..1322)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1982..2773,2774..2842))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2605..2624
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2843..2947)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3015..3033
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
       61 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
      121 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
      181 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
      241 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
      301 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
      361 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgattacgc caagctctaa
      421 tacgactcac tataggggaa agctagcttg ggatctcgag actagcaata acaaaatggt
      481 atgttaatat ggactaaagg aggcttttaa ggacacgtaa tattgagtcg accgtgtttt
      541 tgatatcagt agttagtgaa gttgaattgc atttacaaac tttttatttt gtattgcttt
      601 tcgtcatttt aatagggtag agttagaacc aagaccgtca agcgtgcttc taaggctttg
      661 attgaacgtt actatccaaa gttgactttg gatttccaaa ccaacaagag actttgtgat
      721 gaaatcgcca ctatccaatc caagagattg agaaacaaga ttgctggtta caccacccat
      781 ttgatgaaga gaatccaaaa gggtcggaat tcgtaatcat gtcatagctg tttcctgtgt
      841 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      901 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      961 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     1021 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1081 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1141 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1201 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1261 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1321 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1381 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1441 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1501 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1561 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1621 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1681 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1741 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1801 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1861 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1921 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1981 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2041 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2101 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2161 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2221 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2281 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2341 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2401 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2461 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2521 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2581 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2641 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2701 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2761 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2821 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2881 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2941 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     3001 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctc
//
