LOCUS       sequence-19434-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Park HK, Zeng C
  TITLE     Construction of an XcmI-generated T vector bearing green fluorescent
            protein marker for direct cloning of PCR products.
  JOURNAL   Anal Biochem. 2007 Jan 1;360(1):144-5. doi: 
            10.1016/j.ab.2006.10.031. Epub 2006 Nov 7.
  PUBMED    17113027
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2006.10.031"; journalName: "Anal Biochem"; date: 
            "2007-01-1- 1"; volume: "360"; issue: "1"; pages: "144-5"
COMMENT     Sequence Label: pGT2
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(71..99)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     694..714
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgcaggtcga ctctagagga tccagggttc cggtggaaaa atgagtaaag gagaagaact
       61 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      121 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      181 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactc tgacgtatgg
      241 tgttcaatgc ttttcccgtt atccggatca tatgaaacgg catgactttt tcaagagtgc
      301 catgcccgaa ggttatgtac aggaacgcac gatatctttc aaagatgacg ggaactacaa
      361 gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaagg
      421 tattgatttt aaagaagatg gaaacattct cggacacaaa ctcgagtaca actataactc
      481 acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat
      541 tcgccacaac attgaagatg gatccgttca actagcagac cattatcaac aaaatactcc
      601 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgccct
      661 tttgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc
      721 tgggatacac tgggcatgga tgagctctac aaccacacta gtgctggcgg ccgcgaattc
      781 tt
//
