LOCUS       sequence-194348-        9285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector encoding C-terminally FLAG tagged mouse PERK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Taniuchi S, Miyake M, Tsugawa K, Oyadomari M, Oyadomari S
  TITLE     Integrated stress response of vertebrates is regulated by four 
            eIF2alpha kinases.
  JOURNAL   Sci Rep. 2016 Sep 16;6:32886. doi: 10.1038/srep32886.
  PUBMED    27633668
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-09-16"; volume: "6:32886. doi"; pages: " 
            10.1038/srep32886"
COMMENT     Sequence Label: pMXs-mPERK-FLAG-IP
FEATURES             Location/Qualifiers
     source          1..9285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    7..469
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(63..80)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     290..309
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             483..1085
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MVTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     982..1002
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1256..1725
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1855..1872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2026..2614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2106..2125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2778..3569,3570..3638))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3401..3420
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3639..3743)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3773..4366
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4429..4786
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4845..5261
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5191..5213
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5229..5245
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5271..5645
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5583..5599
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      5704..5713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9085..9150
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
       61 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
      121 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
      181 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
      241 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
      301 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
      361 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
      421 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
      481 ccatggttac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
      541 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
      601 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
      661 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
      721 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
      781 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
      841 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
      901 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
      961 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     1021 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     1081 cctgagtcga cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
     1141 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
     1201 aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa
     1261 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     1321 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     1381 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
     1441 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     1501 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     1561 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     1621 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     1681 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcacatgc agcatgtatc
     1741 aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt tgcattaatg
     1801 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1861 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2461 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2581 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2701 aaggatcttc acctagatcc ttttgcggcc ggccgcaaat caatctaaag tatatatgag
     2761 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2821 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2881 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2941 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3001 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3061 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3121 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3181 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3241 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3301 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3361 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3421 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3481 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3541 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3601 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3661 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3721 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc ataatgaaag
     3781 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     3841 atacataact gagaatagaa aagttcagat caaggtcagg aacagatgga acagctgaat
     3901 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     3961 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     4021 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
     4081 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     4141 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     4201 gcccacaacc cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     4261 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     4321 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
     4381 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggccagc
     4441 aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg
     4501 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg
     4561 aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc
     4621 gacctgagtc caaaaatccc gatcgttttg gactctttgg tgcacccccc taataggagg
     4681 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt
     4741 tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt
     4801 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca
     4861 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
     4921 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
     4981 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
     5041 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
     5101 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
     5161 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
     5221 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga
     5281 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta
     5341 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
     5401 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt
     5461 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct
     5521 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg
     5581 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta
     5641 gactgccgga tctagctagt taattaagga tcccagtgtg gtggtacggg aataattcct
     5701 gcagccacca tggagcgcgc cacccggccc gggccgcgcg cgctgctgct gcttctgttc
     5761 ctgctgctgg gctgcgcggc ggggatctcg gcggtcgcgc ccgcccgcag tttgcttgct
     5821 cccgcgtcgg agacagtgtt tggcttaggg gcagcggccg ccccgacttc ggccgcgcgg
     5881 gtgcctgcgg tggcaacggc cgaagtgacc gtggaggacg ccgaggcatt gccggctgcc
     5941 gctggcgaac cggagtcacg cgcgacggag cccgatgacg acgtggaact gcggcctcgc
     6001 ggcaggtcct tggtaatcat cagcacttta gatggacgaa tcgctgcact ggatgccgag
     6061 aatgatggga aaaagcagtg ggatttggac gtggggtctg gttccttggt ttcatctagc
     6121 ctcagcaagc cagaggtgtt tgggaacaag atgatcatcc cctccctgga tggagacctc
     6181 ttccagtggg accgggaccg agagagcatg gaggccgtcc ccttcacggt ggagtccctg
     6241 ctcgaatctt cctacaagtt tggagatgat gttgttctgg ttggagggaa atctctgact
     6301 acatacggac tcagtgctta cagtggaaag ctgaggtata tctgttctgc cttgggatgt
     6361 cgccgatggg atagtgatga aatggaagaa gaggaagaca tcttgcttct gcagcgtacg
     6421 cagaagactg tgcgagctgt cgggcctcga agcggcagtg agaagtggaa tttcagtgtt
     6481 ggccactttg aacttcggta tattccagac atggaaacta gagccggatt cattgaaagc
     6541 acctttaaac cgggtggaaa caaagaagac tctaaaatta tttcagatgt ggaagaacaa
     6601 gaagccacca tgctggacac agtgataaaa gtttccgttg ctgattggaa ggtcatggcg
     6661 tttagtagga agggaggccg cctggaatgg gagtaccagt tttgtactcc catcgcgtcc
     6721 gcctggctgg tgagggatgg caaggtcatc cccatcagcc tgtttgatga tacaagttac
     6781 acagccagcg aagaagcctt gggagacgaa gaagacattg tagaggctgc tcggggagcc
     6841 acagagaaca gcgtgtactt agggatgtac agaggccagc tgtacctgca gtcgtccgtc
     6901 agggtctcag aaaagttccc tacaagccca aaggccttgg agtctgtaaa tggcgaaaat
     6961 gcaattattc ctctgccgac gatcaaatgg aagcccttaa tccattctcc ttctaggact
     7021 cctgtcttgg ttgggtctga tgaatttgac aaatgtctaa gtaatgataa gtattcccac
     7081 gaagaataca gtaatggtgc actttcaatc ctccagtatc catacgataa cggttactat
     7141 ctgccatact acaagagaga aaggaataag cggagcacgc agatcacagt caggttcctg
     7201 gacagccccc actacagcaa gaacatccgc aagaaggacc ctatcctcct gctgcactgg
     7261 tggaaggaga tattcgggac gatcctgctt tgcatcgtag ccacgacctt catcgtgcgc
     7321 aggcttttcc atcctcagcc ccacaggcag cggaaggagt ctgaaactca gtgccagact
     7381 gaaagtaaat acgactccgt gagtgccgat gtcagtgaca acagctggaa tgacatgaag
     7441 tactcaggat acgtatcccg atatctaaca gattttgagc caattcagtg catgggtcgt
     7501 ggtggctttg gcgttgtctt tgaagctaaa aacaaagtag atgactgcaa ttacgctatc
     7561 aagaggatcc ggctccccaa cagggagttg gcacgggaga aggtaatgcg ggaagttaaa
     7621 gccttggcta agctggaaca cccaggcatt gtgaggtatt tcaacgcctg gctggaaacc
     7681 ccaccagaga agtggcaaga agagatggat gagatctggc tcaaagacga aagcacagac
     7741 tggccgctca gctcccctag cccgatggat gccccatctg ttaagatccg aaggatggat
     7801 cctttctcta caaaagagca gatcgaagtc atagctcctt ctcctgaaag aagtcggtct
     7861 ttctcggtgg gcatttcctg tggccagaca agctcatcgg agagccagtt ctctcccctg
     7921 gagttctcag ggacagactg cggagacaac agtgactcag cggacgcagc ctacaacctc
     7981 caggacagtt gcctgacgga ctgcgaggac gtggaagatg gcaccgtgga cggcaatgac
     8041 gagggacact cctttgaact ttgtccgtcc gaagcttctc cctatacccg gtcaagggaa
     8101 ggaacgtcct cctccatagt gtttgaggac tctggctgcg gcaacgcgtc cagtaaggag
     8161 gagcccagag ggaaccggct gcatgatggc aaccattatg ttaataagct aactgatctc
     8221 aagtgctcca gcagcaggtc ttcttcagaa gccaccacct tgtctacctc ccctaccagg
     8281 ccaaccactc taagcttgga tttcaccaag aacactgtgg gccagctcca gcccagctcc
     8341 cccaaggtgt atctgtacat tcagatgcag ctgtgcagga aggagaacct caaagactgg
     8401 atgaaccggc gctgcagctt ggaggaccgg gagcacggcg tgtgcctgca catcttcctg
     8461 cagatcgcag aggcagtgga gttcctgcac agcaagggac tcatgcacag ggacctcaag
     8521 ccttccaaca tattcttcac aatggatgat gtggtcaagg ttggggactt tggactggtg
     8581 actgctatgg accaagatga agaagagcag actgtactga ctccaatgcc agcctatgct
     8641 acgcacacgg gacaagtagg gaccaagcta tacatgagcc cagagcagat tcatggaaac
     8701 aactactccc ataaagtgga catcttctct ttaggcttga ttctgtttga actcctctac
     8761 ccattcagca cccagatgga acgagtccgg attttaactg atgtcagaaa tctcaagttt
     8821 cctctactgt tcactcagaa atatccccaa gagcatatga tggttcaaga catgctctct
     8881 ccatccccca cggagcggcc tgaagccaca gacatcattg aaaatgccat atttgagaac
     8941 ttggagtttc ccgggaaaac ggttctgaga cagcggtccc gctccatgag ttcatctgga
     9001 acaaaacatt ccagacagcc cagctgctcg tacagcccac tgcctggcaa ctgcaggcct
     9061 cgactcggtc gcgaggccaa ggccgactac aaagaccatg acggtgatta taaagatcat
     9121 gacatcgact acaaggatga cgatgacaag tagtgactgc ggccgcgaag gatctgcgat
     9181 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     9241 ggaggggtcg gcaattggcc gctacgtaaa ttccgccccc ccccc
//
