LOCUS       sequence-194362-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Reverse Transcriptase gene (3'-5' exonuclease 
            deficient mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Ellefson JW, Gollihar J, Shroff R, Shivram H, Iyer VR, Ellington AD
  TITLE     Synthetic evolutionary origin of a proofreading reverse 
            transcriptase.
  JOURNAL   Science. 2016 Jun 24;352(6293):1590-3. doi: 10.1126/science.aaf5409.
  PUBMED    27339990
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-24- 24"; volume: "352"; 
            issue: "6293"; pages: "1590-3"
COMMENT     Sequence Label: pET_RTX_(exo-)
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             23..45
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgattct
       61 tgacacggat tacatcacgg aagacggcaa gccggttatc cgtattttca agaaagaaaa
      121 cggcgaattc aagattgaat acgatcggac atttgaaccg tacctgtacg ctctcctcaa
      181 ggatgatagc gcaatcgaag aagtgaaaaa aatcaccgca gagcggcatg gcacagtggt
      241 aacagttaag cgggtcgaga aagtgcagaa gaagttctta ggccggccag tcgaagtatg
      301 gaaattatac ttcacacatc cacaggacgt tccggcgatc atggataaga ttcgggagca
      361 tccggcggta atcgatatct atgaatacga tattccgttc gctattcgct accttattga
      421 caaaggttta gttccaatgg agggtgatga ggaacttaaa ctgttagcat tcgatatcga
      481 aacactttat cacgaaggtg aagagtttgc cgaaggtccg attttaatga tctcgtacgc
      541 cgatgaagaa ggcgcacgcg taattacgtg gaaaaatgtg gacctcccgt acgtagacgt
      601 agtgagcact gagcgcgaga tgattaaacg tttccttcgg gtagtaaaag aaaaagaccc
      661 agacgtgctg attacgtatg acggcgacaa ctttgatttt gcctatctca agaagcgttg
      721 cgaaaagtta ggcattaatt tcgccctggg tcgggacggt tcagagccga aaattcagcg
      781 gatgggcgac cgctttgctg tg
//
