LOCUS       sequence-194372-       12715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of LIPS M1+TE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12715)
  AUTHORS   Yuzawa S, Deng K, Wang G, Baidoo EE, Northen TR, Adams PD, Katz L, 
            Keasling JD
  TITLE     Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges 
            in Modular Polyketide Synthases and Its Application for Short-Chain 
            Ketone Production.
  JOURNAL   ACS Synth Biol. 2017 Jan 20;6(1):139-147. doi: 
            10.1021/acssynbio.6b00176. Epub 2016 Sep 6.
  PUBMED    27548700
REFERENCE   2  (bases 1 to 12715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00176"; journalName: "ACS Synth Biol"; date: 
            "2017-01-20- 20"; volume: "6"; issue: "1"; pages: "139-147"
COMMENT     Sequence Label: Psy074
FEATURES             Location/Qualifiers
     source          1..12715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(36..54)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      50..97
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      134..589
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(221..240)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     431..452
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(681..1496)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(681..1496)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1404..1423
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1488..1507
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1618..2206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2107..2126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2360..2377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2392..2531
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2457..2476)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2617..2639
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2633..2824)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3633..4715)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4677..4696
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4716..4793)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4999..5018
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5106..5125
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5106..5124
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5125..5149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5164..5186
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5206..5223
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5233..5250
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             12681..12698
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
       61 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      121 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      181 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      241 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      301 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
      361 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
      421 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
      481 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
      541 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
      601 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      661 atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      721 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      781 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
      841 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
      901 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
      961 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     1021 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     1081 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     1141 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
     1201 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     1261 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     1321 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
     1381 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     1441 atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcataaca
     1501 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgacca aaatccctta
     1561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1741 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     1801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     2401 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca
     2461 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     2521 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2581 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2641 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     2701 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     2761 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     2821 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac
     2881 gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt
     2941 gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc
     3001 aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg
     3061 cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg
     3121 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
     3181 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
     3241 gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggggccgcca
     3301 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg
     3361 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc
     3421 tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga
     3481 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc
     3541 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat
     3601 gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3661 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3721 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
     3781 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
     3841 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat
     3901 cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
     3961 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc
     4021 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc
     4081 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag
     4141 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc
     4201 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg
     4261 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca
     4321 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
     4381 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
     4441 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg
     4501 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
     4561 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
     4621 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
     4681 tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct
     4741 tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc
     4801 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
     4861 gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc
     4921 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     4981 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
     5041 gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc
     5101 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat
     5161 aattttgttt aactttaaga aggagatata catatgggca gcagccacca ccaccaccac
     5221 cacagcagcg gcctggtccc gcgtggttcc cacatggcag gtccaccgcc gttcccgcgt
     5281 cgtcgtggcc cgagcggccg tcgtcgttgc ggcggtcgtg ctaccccggg tagcgtccgt
     5341 gaccgtacgg gtcgtcgtcc ggcagccgtt ccgagccgtg cagtttgcgc agcggatctg
     5401 tgcgaagaaa atgacgacgg ctcgaagaat gtttctgaac atcgcggtag cgcaggtggt
     5461 tccgtgctgt ttccacgtac cggcaccgtc ctgccgtggg tactgaccgg tcctggcgca
     5521 gcggcggttc gcgcacgctc cgaagcactg cgcacgcacc tgcgtgcgag caccgagtgg
     5581 tcccctgcgg gcgtcggtca ggcgctgctg gccggtacgg gtgcgggtgc cgatacccac
     5641 cgtgccgttg ttctggcagg cgaccgtgcc cagaccctga acgcattggc agcgctgagc
     5701 gcaggcgcag accacccggc agttttcacc agcactcgtg cggatgcaag cccggctggc
     5761 ccggtgtttg tgttcccggg tcaaggctcg cagtggaccg gtatggctcg tgaactgctg
     5821 gactccgcac cggttttcgc gcgtaagctg cacgactgtg cagacgcgtt tgccccgtac
     5881 ctgggccaca gcctgctgga tagcgtcacc ggtgcagcag gtggtccaga gcctgttggc
     5941 gcggacgtcg tccaaccggc gctgttcgcc gttatggttg cgctgactga tctgtggaac
     6001 gcggctggcg ttgcaccggg tgcactgctg ggtcactccc tgggtgaact ggcagccgcg
     6061 catgtcgcgg gtgttctgtc cctggacgat tctgctcgcg tcgtggcgcg ttggagccaa
     6121 gcgcaggcta cgttggcggg tcgtggtgac atggtcagcg ttctgttgcc tgcggatgaa
     6181 ttggcggacc tgctggaccg ccgttggccg ggtcgcttgg ttgtggcggt tgaaaacggt
     6241 ccaggtagcg cggtcgcgag cggtgacctg gacgctgcgg cggaactggt cgcacacctg
     6301 accgccgaag gtatccacgc gcgtcgcgtt gacgtgggcc tggcggctca cagcccgcac
     6361 attgacgcga tcctgccacg tattcgcgcg gacatcgcgc cgattcgtgc gcatacgccg
     6421 agcatcccgg tttattcggc gctgcatggt ggtgcactgg atggcacgcc aatggacgcg
     6481 gcgtactggt gtcgtaatct gcgctccact gtacgtttcg cggacgcgac ccgtgcagcc
     6541 ctggaggcag gccataccac gtttgtggag gtaagcccac atccggtcct gactacggcg
     6601 atggaggtga gcgcaacccg tgccgcgcac gcagcaactg tcctgggtac gctgcgccgt
     6661 ggtgagggtg gtccgagccg cttcctggcg agcctggccg aactgcatgt cagcggtggt
     6721 gatgccgatc tgcgtacggt tctgccggcg agccaggcgg ctggcttgcc ggaaaccgtt
     6781 ctgacggcgg gtccgcgtgg cgagagcgcg gatggcgact ctcgtcatga ggttctgtgc
     6841 gcacgcctgg caccgctgga cccagcggag cgtcgtgccc agctgctgac tgttgttcgt
     6901 gaaagcgcag ctgccgcgct ggacggcgac gatcaaggta gcattgacgg tcgtcgcacg
     6961 ttccgtgacc tgggtatcac gtcgctggca gcggtgggca tccgtgatcg cctgcattcc
     7021 gcaaccggtc tgcgtctgtc tccgaccgtt gtgtttgatc atccgacccc ggacgcactg
     7081 gcggcacact tggacaccga actgttcggc acgggcgcag atgccgagcc ggcaccagct
     7141 gcgggtggtc gtgcggtgcc gcatgacgaa ccaattgcga tcgtgggtat ggcgtgccgt
     7201 taccctggcg gcgttggtgc accggccgac ctgtggcgta ccgttctggc cggtgtcgac
     7261 gcagttggtc cgctgccggc tgatcgtggc tggaatattg cggacggtta cgatccggag
     7321 ctggcgggtc ctggtcgttt tagccagcgt gagggcggct ttctgcacga cgcagctgaa
     7381 tttgatgcgg agttctttgg tattagcccg cgtgaggcat tggcgatgga cccgcagcaa
     7441 cgtttggctc tggaaagcgc ctgggaagcg attgaggatg cgggtctgga cgcccatagc
     7501 ctgcgtggca gccgtactgg cgtttttctg ggcttgatta cccaggatta tggtcctcgt
     7561 gcgggtgagc cgaccacgcg tgcaggtgcg gtggagggtc acctgttcct gggtagcact
     7621 ggcagcgtcg caagcggtcg tctgagctat accttgggtc tggaaggtcc gtctttgacg
     7681 attgatacgg catgttcgag cagcctggtg gcactgcacg aagcatgtca agcgctgcgt
     7741 accggtgatt gcgacatggc tctgactggt ggtgtgacgg tcatgccgag caccggcatg
     7801 ttggtcgagt tcagccgtca gcgtggtctg tcgcctgacg gccgttgtaa agccttttct
     7861 gcatctgccg acggttttgg tctggcggaa ggtgtcggta tgctggtggt tgagcgtctg
     7921 agcgatgcgc gtcgtctggg ccatcgtgtg ctggcggtgg tgcgcggttc tgcggttaac
     7981 caagatggcg cgagcaatgg cctgtcggcg cctagcggcc cagcacaaca gcgcgttatt
     8041 cgccaggcgc tggtcaacgc tggcgtccaa gcatcccaag tggacgttgt cgaagcacat
     8101 ggcaccggta cgaaactggg cgatccgatt gaggctcaag ccctgcaagc gacctatggc
     8161 cagggccgtc cggctgagcg cccgttgtgg ttgggttctc tgaagtccaa tatcggccac
     8221 gcgcaagcgg cagcgggtgt gggcggtgtt atcaaaatgg tcatggcgtt gcgtgaaggc
     8281 gtcctgccac cgaccctgca cgcagacgag ccgagcccgc atattgactg gtcggcgggt
     8341 caggttcgtc tgctgaccga ggaacgcgag tggccggagg caggtcaccc tcgccgtgcg
     8401 gcagtttcga gcttcggtgt tagcggtacc aacgcacatg tgattctgga agctgcaccg
     8461 ggtacgggtg gtgcgccaga agtttcggac ggtgtcctgg gtagcgcgcc tgaaacggtc
     8521 ccgtgggtgc tgagcgctgc aagccctgac gcattgcgtg cacaagcaga gcgtctgcgc
     8581 ggtcatgtgg cggagcgtcc gggtctggct tccgccgatg tcgcgtttgc gctggcgacc
     8641 cgtcgtaccg cgctggaata tcgcgcggtg gcggttggtg cggagcgcga cgagctgctg
     8701 gataccttgg acgcgctgag cgccggtcgt ccggcaccgc gtgctgtacc gggtgacgcg
     8761 gctgcgcata gccgtcgtcc ggttttcgtc tttccgggtc agggtagcca gtgggcaggt
     8821 atggcggttg aactgctgga cagcagcccg gtttttgcgg acagcatgca cgcatgttcc
     8881 gaggccctga atgaatttgt tgactggaac ctgctggaag ttctgcgtag cggtgacgaa
     8941 gagctgtcta accgtgttga tgtcgtccaa ccggtgctgt gggcagttat ggtgagcctg
     9001 gcagctctgt ggcaagcgtg tggcgtccgt cctgcggcgg ttgtgggtca cagccaaggt
     9061 gagattgcag ctgccgttgt cgcaggtgca ctgagcctgc gtgatggtgc ccgcgttgtt
     9121 gcattgcgta gcgcagtgat cgcgcgtctg ctggcaggta agggtgcgat ggcgagcgtg
     9181 gctctggcgt ctgacaccgt tcgtgagcgc ctgaccccgt gggaaggtcg tctgtctctg
     9241 gcagcggtca atggtccgag cagcagcgtt gtttgcggcc atctggatgc actggacgag
     9301 ttcgttagcg cgttggagca cgatggcgtg cgtgtgcgtc gcatcgcggt tgactacgca
     9361 agccatagcg tgttcgtgga gcaggcagaa gaagagctgc gtaatgtcct gaccgaggtg
     9421 agccctttgc cgggtcaagt ccctttctac agcaccgtga ccggtgcggt tctggatacc
     9481 acgactctgg acgccggcta ctggtatcgt aacttgcgtc agacggttcg tttcgaagaa
     9541 accgtgcgtg agctgacgcg ccgtggccac gacgcgttca tcgaggtgtc ggctcatccg
     9601 gttctgaccg tcggcattca ggataccttg gaagccaccg gcacccgcca tgcagtctgc
     9661 ggtacgctgc gtcgtggtga gggcggtgcg cagcgtttgc tgaccagcct gggtgaagcg
     9721 tgggttgccg gcattgcggt ggactggagc cgcttgacgc cgacgacgac cgctgtccaa
     9781 ctgccgacct acgcatttca gcatcagcgt tactggctgg atagcaccac tgcaaacact
     9841 ggtgaccgtc cggcagcgga ccgtgacacc gcattttggg aagctgtgca gcacaccgac
     9901 ctggacgcct tcgctgcaga attggacatt gccccggatg cgccgttggg caccgtcttg
     9961 ccggctctgg ctgactggcg tcaacgcctg cgtacggcag cggctgttga cgcatggcgt
    10021 taccgcaccg cctttaaacg tctgccagat gcgccaggtg caccagtcct gacgggcagc
    10081 tggctggccg tagttccggt gcgtcacctg gatgatccga gcgttaccac tagcctggat
    10141 gcagttgcta aagcgggtgc ggaagtcgtt cagttggcaa tcgaagatgc ggacgcggac
    10201 gttgatcgtc tgactgagcg cttgcgtggc ctggttgccg gtctgggtgc cgcgccggcg
    10261 ggcattatga gcttcctggg tctggatgaa gagcgtcatc gtgaccaccc ggcgatgccg
    10321 agcggtctgg ccaccagctt ggcgctggtc cgcgccttgg gtcgtgcggg catcggtgca
    10381 ccgctgtgga tggttacgcg tgaggcagtg gcagcgggtc aagacacgca cccgcatgcg
    10441 cctctgggta gcctgatctg gggtctgggc caagtgacgg ctctggagca cgcagatcgc
    10501 tggggtggtc tgatcgatct gccgggtgtg tgtgatgcgc gcgttgcccg catgctgtgc
    10561 gcgggtctga gcggccgtgg tgccgaagat cagctggccc tgcgtccgag cggcactttc
    10621 gtccgccgtc tggcgcatat ccctggcgag caacgtgcag cacgtcgtag ctggcaacca
    10681 cgtggtacgg tgattgtcac cggtggtacg ggtgcgctgg gtgcagtcct ggcccgctgg
    10741 ttggctaccg aggacgcgga gcacctggtg ctgaccggcc gtcgtggcgc ggacgcccct
    10801 ggcgcggagc gtttgcgtga cgagctggtc gctacgggcg ctcgtgtcac gctggcggcg
    10861 tgcgacgtgg cagatcgcaa agccgtcgcc gcattgctgg acgaactggc tgcggacggc
    10921 gagactgttc gcgcagttct gcacgctgcg ggtgtcgccg atctgacgtc gctggagaat
    10981 accggtccag aagcgttcgc ggcaggcgtg gccgcgaagg tcgatggtgc actgcacctg
    11041 accgaactgt tggatcacga ttcgctggat gcgtttgtgt tgttcagcag cattgcgggt
    11101 gtttggggtt ccggcgacca cggcgcgtat gcggctgcga acgcatttct gaatgcgttg
    11161 gcagagtaca atcgtgcacg cggtatcccg accacgagca tcgcatgggg cgtttggaac
    11221 gcgtttggcg tcgagggtgc aggcggtatc agcgaggcgg ttgatttgga ccagctgcat
    11281 cgtcgcggcc tgccgctgat tgaaccagag ctgggtctga ctgcactgcg tcgcgctcta
    11341 gaccgtgacg aaacggtgct gacggttgct ccggttgcct gggagcgctt ctttccgctg
    11401 ttctccgctg cacgtccgcg tccgttgttt gaggacttgc cgcaagtgcg tgccctgagc
    11461 gcacctgtcc cgacgacggc gggtccggcc gtggaaccag gtcgccgtgg tagcggcctg
    11521 ggcgatttgc ctctgacgga tcgcgatagc gcgctgctgg ccttggtccg cggtgagagc
    11581 gcatccgtgc tgggttacga gcgtccagat cgcctggacc cggaccgtgc gctgcgtgat
    11641 gtgggtttcg atagcctgac ggcgatggaa ctgcgtaacc gtctggctac cgcgaccggc
    11701 ctgacgctgc ctgcggccct ggtgtttgat cacccgaccc cactggcgat cgcggcgtat
    11761 ctgaaagccg agctgacgag ccagctggac tccggtacgc ctgcacgtga agctagctct
    11821 gcactgcgtg acggctaccg ccaagcgggt gtgagcggcc gtgttcgtag ctacctggat
    11881 ctgttggcag gtctgtcgga tttccgcgaa cattttgatg gtagcgatgg ttttagcctg
    11941 gatctggttg atatggcaga tggtccgggt gaggtgaccg ttatttgctg cgcgggtacg
    12001 gctgcgatct ctggtccgca tgagttcacc cgtctggcag gtgccctgcg cggcattgca
    12061 cctgttcgtg cggtgccgca gccgggttac gaagaaggcg aaccgctgcc ttctagcatg
    12121 gcggctgttg cagctgtgca ggctgatgct gtcattcgta cgcaaggcga taagccgttc
    12181 gtggttgcgg gtcactcggc aggcgcgctg atggcgtacg cgctggcgac cgaactgctg
    12241 gatcgtggtc acccgcctcg cggtgttgtc ctgattgatg tgtacccgcc tggtcatcag
    12301 gacgcgatga acgcgtggct ggaggaactg acggcaacgt tgttcgaccg tgaaactgtt
    12361 cgtatggacg acacccgtct gaccgcgttg ggtgcttacg accgcctgac gggtcaatgg
    12421 cgtcctcgcg aaaccggttt gccgaccctg ttggttagcg cgggtgaacc aatgggcccg
    12481 tggccggacg atagctggaa accgacctgg cctttcgagc acgacaccgt tgcggtcccg
    12541 ggtgatcatt tcacgatggt tcaagaacat gctgatgcga ttgcccgtca cattgacgcc
    12601 tggctgggtg gcggcaacag ctgataagct tataggatcc gaattcgagc tccgtcgaca
    12661 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaa
//
