LOCUS       sequence-194384-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Taq DNA polymerase under WT T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD
  TITLE     Compartmentalized partnered replication for the directed evolution 
            of genetic parts and circuits.
  JOURNAL   Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. 
            Epub 2017 Nov 9.
  PUBMED    29120463
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.119"; journalName: "Nat Protoc"; date: 
            "2017-12"; volume: "12"; issue: "12"; pages: "2493-2512"
COMMENT     Sequence Label: pACYC-Taq
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      459..506
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cccttacgag gaggcccagg ccttcattga gcgctacttt cagagcttcc ccaaggtgcg
       61 ggcctggatt gagaagaccc tggaggaggg caggaggcgg gggtacgtgg agaccctctt
      121 cggccgccgc cgctacgtgc cagacctaga ggcccgggtg aagagcgtgc gggaggcggc
      181 cgagcgcatg gccttcaaca tgcccgtcca gggcaccgcc gccgacctca tgaagctggc
      241 tatggtgaag ctcttcccca ggctggagga aatgggggcc aggatgctcc ttcaggtcca
      301 cgacgagctg gtcctcgagg ccccaaaaga gagggcggag gccgtggccc ggctggccaa
      361 ggaggtcatg gagggggtgt atcccctggc cgtgcccctg gaggtggagg tggggatagg
      421 ggaggactgg ctctccgcca aggagtgata gaattagact agcataaccc cttggggcct
      481 ctaaacgggt cttgaggggt tttttgctga aacctcaggc atttgagaag cacacggtca
      541 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac
      601 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg
      661 cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta
      721 aaaaaattac gccccgccct g
//
