LOCUS       sequence-194389-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Taq DNA polymerases with a premature stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD
  TITLE     Compartmentalized partnered replication for the directed evolution 
            of genetic parts and circuits.
  JOURNAL   Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. 
            Epub 2017 Nov 9.
  PUBMED    29120463
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.119"; journalName: "Nat Protoc"; date: 
            "2017-12"; volume: "12"; issue: "12"; pages: "2493-2512"
COMMENT     Sequence Label: pACYC.Taq.1Amb
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      470..517
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gagctagcca tcccttacga ggaggcccag gccttcattg agcgctactt tcagagcttc
       61 cccaaggtgc gggcctggat tgagaagacc ctggaggagg gcaggaggcg ggggtacgtg
      121 gagaccctct tcggccgccg ccgctacgtg ccagacctag aggcccgggt gaagagcgtg
      181 cgggaggcgg ccgagcgcat ggccttcaac atgcccgtcc agggcaccgc cgccgacctc
      241 atgaagctgg ctatggtgaa gctcttcccc aggctggagg aaatgggggc caggatgctc
      301 cttcaggtcc acgacgagct ggtcctcgag gccccaaaag agagggcgga ggccgtggcc
      361 cggctggcca aggaggtcat ggagggggtg tatcccctgg ccgtgcccct ggaggtggag
      421 gtggggatag gggaggactg gctctccgcc aaggagtgat agaattagac tagcataacc
      481 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa
      541 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc
      601 ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg
      661 ccattcatcc gcttattatc acttattcag gcgtagcaac c
//
