LOCUS       sequence-19439-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Schofield MJ, Brownewell FE, Nayak S, Du C, Kool ET, Hsieh P
  TITLE     The Phe-X-Glu DNA binding motif of MutS. The role of hydrogen 
            bonding in mismatch recognition.
  JOURNAL   J Biol Chem. 2001 Dec 7. 276(49):45505-8.
  PUBMED    11602569
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Dec 7. 276(49):45505-8."
COMMENT     Sequence Label: pES E38A
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..103
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttcccctcta gaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca
       61 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatga gtgcaataga
      121 aaatttcgac gcccatacgc ccatgatgca gcagtatctc aggctgaaag cccagcatcc
      181 cgagatcctg ctgttttacc ggatgggtga tttttatgca ctgttttatg acgacgcaaa
      241 acgcgcgtcg caactgctgg atatttcact gaccaaacgc ggtgcttcgg cgggagagcc
      301 gatcccgatg gcggggattc cctaccatgc ggtggaaaac tatctcgcca aactggtgaa
      361 tcagggagag tccgttgcca tctgcgaaca aattggcgat ccggcgacca gcaaaggtcc
      421 ggttgagcgc aaagttgtgc gtatcgttac gccaggcacc atcagcgatg aagccctgtt
      481 gcaggagcgt caggacaacc tgctggcggc tatctggcag gacagcaaag gtttcggcta
      541 cgcgacgctg gatatcagtt ccgggcgttt tcgcctgagc gaaccggctg accgcgaaac
      601 gatggcggca gaactgcaac gcactaatcc tgcggaactg ctgtatgcag aagattttgg
      661 ctgaaatt
//
