LOCUS       sequence-194391-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP under WT T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD
  TITLE     Compartmentalized partnered replication for the directed evolution 
            of genetic parts and circuits.
  JOURNAL   Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. 
            Epub 2017 Nov 9.
  PUBMED    29120463
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.119"; journalName: "Nat Protoc"; date: 
            "2017-12"; volume: "12"; issue: "12"; pages: "2493-2512"
COMMENT     Sequence Label: pACYC-GFPM2
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..791
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(105..133)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     728..748
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 taattttgtt taactttaat tctagagtca cacaggacta ctagatgggg atgaccatga
       61 ttacgccaag ctttatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      121 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      181 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      241 catggccaac acttgtcact actttcgcgt atggtcttca atgctttgcg agatacccag
      301 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      361 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aaatttgaag
      421 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      481 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      541 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
      601 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      661 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
      721 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
      781 tatacaaata gaattagact agcataaccc cttggggcct ctaaacgggt cttga
//
