LOCUS       sequence-194394-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP with a premature stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD
  TITLE     Compartmentalized partnered replication for the directed evolution 
            of genetic parts and circuits.
  JOURNAL   Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. 
            Epub 2017 Nov 9.
  PUBMED    29120463
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.119"; journalName: "Nat Protoc"; date: 
            "2017-12"; volume: "12"; issue: "12"; pages: "2493-2512"
COMMENT     Sequence Label: pACYC.GFP1Amb
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..789
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT*GKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(103..131)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     726..746
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 attttgttta actttaattc tagagtcaca caggactact agatggggat gaccatgatt
       61 acgccaagct ttatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      121 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      181 gcaacatagg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      241 tggccaacac ttgtcactac tttcgcgtat ggtcttcaat gctttgcgag atacccagat
      301 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      361 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa atttgaaggt
      421 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      481 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
      541 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
      601 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      661 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
      721 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
      781 tacaaataga attagactag cataacccct tggggcctct aaacgggtct tgaggg
//
