LOCUS       sequence-194395-         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP with a premature stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD
  TITLE     Compartmentalized partnered replication for the directed evolution 
            of genetic parts and circuits.
  JOURNAL   Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. 
            Epub 2017 Nov 9.
  PUBMED    29120463
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.119"; journalName: "Nat Protoc"; date: 
            "2017-12"; volume: "12"; issue: "12"; pages: "2493-2512"
COMMENT     Sequence Label: pACYC.GFP1Amb
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     453..473
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      524..571
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      574..621
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
       61 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      121 acacgtgctg aagtcaaatt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      181 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
      241 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
      301 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
      361 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
      421 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
      481 gggattacac atggcatgga tgaactatac aaatagaatt agactagcat aaccccttgg
      541 ggcctctaaa cgggtcttga ggggtttttt gcactagcat aaccccttgg ggcctctaaa
      601 cgggtcttga ggggtttttt gcgaattcga tctc
//
