LOCUS       sequence-194397-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses T7 RNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD
  TITLE     Compartmentalized partnered replication for the directed evolution 
            of genetic parts and circuits.
  JOURNAL   Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. 
            Epub 2017 Nov 9.
  PUBMED    29120463
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.119"; journalName: "Nat Protoc"; date: 
            "2017-12"; volume: "12"; issue: "12"; pages: "2493-2512"
COMMENT     Sequence Label: pQE 80L Kan T7WT
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..37
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             56..67
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             86..103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aacaattata atagattcaa ttgtgagcgg ataacaattt cacacagaat tcattaaaga
       61 ggagaaatta actatgagag gatcgcatca ccatcaccat cacggatcca acacgattaa
      121 catcgctaag aacgacttct ctgacatcga actggctgct atcccgttca acactctggc
      181 tgaccattac ggtgagcgtt tagctcgcga acagttggcc cttgagcatg agtcttacga
      241 gatgggtgaa gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc
      301 ggataacgct gccgccaagc ctctcatcac taccctactc cctaagatga ttgcacgcat
      361 caacgactgg tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt
      421 cctgcaagaa atcaagccgg aagccgtagc gtacatcacc attaagacca ctctggcttg
      481 cctaaccagt gctgacaata caaccgttca ggctgtagca agcgcaatcg gtcgggccat
      541 tgaggacgag gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa
      601 cgttgaggaa caactcaaca agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt
      661 tgtcgaggct gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca
      721 taaggaagac tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg
      781 aatggttagc ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga
      841 actcgcacct gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc
      901 tccgatgttc caaccttgcg tagttcctcc taagccgtgg a
//
