LOCUS       sequence-194398-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses T7 RNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD
  TITLE     Compartmentalized partnered replication for the directed evolution 
            of genetic parts and circuits.
  JOURNAL   Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. 
            Epub 2017 Nov 9.
  PUBMED    29120463
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.119"; journalName: "Nat Protoc"; date: 
            "2017-12"; volume: "12"; issue: "12"; pages: "2493-2512"
COMMENT     Sequence Label: pQE 80L Kan T7WT
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc ggtggcgagg
       61 cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc atcgagatgc
      121 tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta gtaggtcaag
      181 actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg
      241 cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag ccgtggactg
      301 gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc
      361 acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg tacaaagcga
      421 ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg
      481 taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag cgtgaagaac
      541 tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg tggaaacgtg
      601 ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt
      661 tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc ccttacaaca
      721 tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt aacgatatga
      781 ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt tactactggc
      841 tgaaaatcca cggtgcaaac tgtgcgggtg tcga
//
