LOCUS       sequence-194400-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses T7 RNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD
  TITLE     Compartmentalized partnered replication for the directed evolution 
            of genetic parts and circuits.
  JOURNAL   Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. 
            Epub 2017 Nov 9.
  PUBMED    29120463
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.119"; journalName: "Nat Protoc"; date: 
            "2017-12"; volume: "12"; issue: "12"; pages: "2493-2512"
COMMENT     Sequence Label: pQE 80L Kan T7WT
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      801..895
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttactcgcag tgtgactaag cgttcagtca tgacgctggc ttacgggtcc aaagagttcg
       61 gcttccgtca acaagtgctg gaagatacca ttcagccagc tattgattcc ggcaagggtc
      121 tgatgttcac tcagccgaat caggctgctg gatacatggc taagctgatt tgggaatctg
      181 tgagcgtgac ggtggtagct gcggttgaag caatgaactg gcttaagtct gctgctaagc
      241 tgctggctgc tgaggtcaaa gataagaaga ctggagagat tcttcgcaag cgttgcgctg
      301 tgcattgggt aactcctgat ggtttccctg tgtggcagga atacaagaag cctattcaga
      361 cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca gcctaccatt aacaccaaca
      421 aagatagcga gattgatgca cacaaacagg agtctggtat cgctcctaac tttgtacaca
      481 gccaagacgg tagccacctt cgtaagactg tagtgtgggc acacgagaag tacggaatcg
      541 aatcttttgc actgattcac gactccttcg gtaccattcc ggctgacgct gcgaacctgt
      601 tcaaagcagt gcgcgaaact atggttgaca catatgagtc ttgtgatgta ctggctgatt
      661 tctacgacca gttcgctgac cagttgcacg agtctcaatt ggacaaaatg ccagcacttc
      721 cggctaaagg taacttgaac ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaaa
      781 agcttaatta gctgagcttg gactcctgtt gatagatcca gtaatgacct cagaactcca
      841 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag
      901 ctagcttggc gagattt
//
