LOCUS       sequence-194427-       10803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes amino acids 1-1000 of HERC2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p6325 pHAGE-P-CMVt-N-HA-GAW-HERC2-F1
FEATURES             Location/Qualifiers
     source          1..10803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      19..607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     508..527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     761..778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1018..1039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1054..1059,1060..1077,1078..1084)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1092..1108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1097..1119
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1116..1132
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1116..1132
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1182..1201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1393..2026
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2073..2198
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2695..2928
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3113..3157
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3450..3567
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3619..3922
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3923..4126
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4077..4097
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4128..4146
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4149..4167
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4177..4201
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4288..4297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4300..4326
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4342..4366
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7368..7392)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7411..7898
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7446..7469)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7831..7850
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7919..8518
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7919..7938)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8415..8435
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8585..9173
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8638..8658)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9056..9067)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9248..9481
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9600..9618)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9686..9790
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9791..9859,9860..10651)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10009..10028)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
       61 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      481 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      541 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      601 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      661 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      721 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      781 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      841 cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      901 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      961 ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     1021 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     1081 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
     1141 gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1201 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     1261 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
     1321 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     1381 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     1441 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     1501 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     1561 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     1621 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     1681 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     1741 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     1801 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     1861 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1921 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1981 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2041 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2101 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2161 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2221 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2281 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2341 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     2401 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     2461 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     2521 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     2581 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     2641 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     2701 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     2761 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     2821 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     2881 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     2941 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     3001 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     3061 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     3121 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     3181 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     3241 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     3301 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     3361 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgccg
     3421 aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     3481 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     3541 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     3601 tggactagtg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     3661 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     3721 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     3781 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3841 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     3901 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     3961 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4021 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     4081 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga
     4141 tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat
     4201 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     4261 cctccggact ctagcgttta aacgctagcc accatggcgt acccatacga tgttccagat
     4321 tacgctgctc gaggttatca aacaagtttg tacaaaaaag ttggcatgcc ctctgaatct
     4381 ttctgtttgg ctgcccaggc tcgcctcgac tccaaatggt tgaaaacaga tatacagctt
     4441 gcattcacaa gagatgggct ctgtggtctg tggaatgaaa tggttaaaga tggagaaatt
     4501 gtatacactg gaacagaatc aacccagaac ggagagctcc ctcctagaaa agatgatagt
     4561 gtcgaaccaa gtggaacaaa gaaagaagat ctgaatgaca aagagaaaaa agatgaagaa
     4621 gaaactcctg cacctatata tagggccaag tcaattctgg acagctgggt atggggcaag
     4681 caaccagatg tgaatgaact gaaggagtgt ctttctgtgc tggttaaaga gcagcaggcc
     4741 ctggccgtcc agtcagccac caccaccctc tcagccctgc gactcaagca gaggctggtg
     4801 atcttggagc gctatttcat tgccttgaat agaaccgttt ttcaggagaa tgtcaaagtt
     4861 aagtggaaaa gcagcggtat ttctctgcct cctgtggaca aaaaaagttc ccggcctgcg
     4921 ggcaaaggtg tggaggggct cgccagagtg ggatcccgag cggcgctgtc ttttgccttt
     4981 gccttcctgc gcagggcctg gcgatcaggc gaggatgcgg acctctgcag tgagctgttg
     5041 caggagtccc tggacgccct gcgagcactt cccgaggcct cgctctttga cgagagcacc
     5101 gtgtcctctg tgtggctgga ggtggtggag agagcgacca ggttcctcag gtccgtcgtg
     5161 acgggggatg ttcacggaac gccagccacc aaagggccag gaagcatccc cctgcaggac
     5221 cagcacttgg ccctggccat cctgctggag ctggctgtgc agagaggcac gctgagccaa
     5281 atgttgtctg ccatcctgtt gttgcttcag ctgtgggaca gcggggcaca ggagactgac
     5341 aatgagcgtt ccgcccaggg caccagcgcc ccacttttgc ccttgctgca aaggttccag
     5401 agcatcattt gcaggaagga tgcaccccac tccgagggcg acatgcacct tttgtctggc
     5461 cctctgagcc ccaatgagag tttcctgagg tacctcaccc ttccacaaga caacgagctt
     5521 gccattgatc tgcgacaaac ggcggttgtt gtcatggccc atttagaccg tctggctacg
     5581 ccctgtatgc ctccgctgtg tagctctccg acatctcata agggatcatt gcaagaggtc
     5641 ataggttggg ggttaatagg atggaaatac tatgccaatg tgattggtcc aatccagtgc
     5701 gaaggcctgg ccaacctggg agtcacacag attgcctgtg cagagaagcg tttcctgatt
     5761 ctgtcacgca atggccgcgt gtacacacag gcctataata gtgacacgct ggccccacag
     5821 ctggtccaag gccttgcctc cagaaacatt gtaaaaattg ctgcccattc tgatggtcac
     5881 cactacctag ccttggctgc tactggagag gtgtactcct ggggctgtgg ggacggcgga
     5941 cggctgggcc atggggacac tgtgcctttg gaggagccta aggtgatctc cgccttctct
     6001 ggaaagcagg ccgggaagca cgtggtgcac atcgcttgcg ggagcactta cagtgcggcc
     6061 atcactgccg agggggagct gtacacctgg ggccgcggga actacggccg gctgggccat
     6121 ggctccagtg aggacgaggc cattccgatg ctggtagccg ggcttaaagg actgaaggtc
     6181 atcgatgtgg cgtgtgggag tggggatgct caaaccctgg ctgtcactga gaacgggcaa
     6241 gtgtggtctt ggggagatgg tgactatggg aaattgggca gaggtggtag tgatggctgc
     6301 aaaaccccaa agctgattga aaagcttcaa gacttggatg tggtcaaagt ccgctgtgga
     6361 agtcagtttt ccattgcttt gacgaaagat ggccaagttt attcatgggg aaaaggtgac
     6421 aaccagagac ttggacatgg aacagaggaa catgttcgtt atccaaaact cttagaaggc
     6481 ttgcaaggga agaaggtgat tgatgtggct gcaggctcca cccactgcct ggctctgact
     6541 gaggacagcg aggtccacag ctgggggagc aacgaccagt gccagcactt tgacaccttg
     6601 cgcgtgacca agccagaacc tgcagcattg ccaggactgg acaccaaaca catagtggga
     6661 attgcctgtg ggcctgccca gagctttgct tggtcatcat gttctgagtg gtccattggc
     6721 ctccgtgtcc cttttgtggt ggacatctgc tcaatgactt ttgagcagct ggatctcctg
     6781 cttcggcagg tgagtgaggg gatggatggc tccgcggact ggcccccgcc ccaggagaaa
     6841 gagtgtgtgg ccgtggcaac gctgaatctt ctacgacttc agttgcatgc tgccattagt
     6901 caccaggttg acccggaatt ccttggttta ggtctgggca gcatcctcct gaacagcctg
     6961 aagcagacgg tggtgaccct ggccagcagt gcgggcgtgc tgagcaccgt gcagtcggcc
     7021 gcccaggccg tgctgcagag tggctggtcc gtgctgctgc ccaccgcgga ggagcgggcc
     7081 cgggcactct ctgctctcct gccctgcgca gtttcaggca atgaagtgaa cataagtcca
     7141 ggtcgtcgat tcatgattga tcttctggtg ggcagcttga tggctgatgg agggttggag
     7201 tcagccttac acgcagccat tactgcagag atccaggata ttgaagccaa aaaagaagca
     7261 cagaaggaaa aagaaattga tgaacaggaa gcgaatgcct caacatttca tagaagcagg
     7321 actccactgg ataaagacct tattaatacg gggatctgtg agtcttgacc caactttctt
     7381 gtacaaagtg gttcgataac gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     7441 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     7501 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     7561 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     7621 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     7681 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     7741 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggg
     7801 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     7861 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     7921 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     7981 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     8041 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     8101 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     8161 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     8221 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     8281 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     8341 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     8401 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     8461 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     8521 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgataga
     8581 tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     8641 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     8701 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     8761 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     8821 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     8881 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     8941 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     9001 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     9061 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     9121 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat
     9181 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     9241 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     9301 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9361 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9421 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9481 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     9541 gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg
     9601 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     9661 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9721 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9781 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     9841 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9901 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9961 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    10021 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    10081 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    10141 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    10201 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    10261 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    10321 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    10381 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    10441 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    10501 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    10561 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    10621 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10681 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10741 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10801 aga
//
