LOCUS       sequence-194431-       10218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes amino acids 950-1750 of HERC2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 10218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p6326 pHAGE-P-CMVt-N-HA-GAW-HERC2-F2
FEATURES             Location/Qualifiers
     source          1..10218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      22..610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     511..530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     764..781
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1021..1042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1057..1062,1063..1080,1081..1087)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1095..1111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1100..1122
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1119..1135
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1119..1135
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1185..1204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1396..2029
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2076..2201
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2698..2931
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3116..3160
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3453..3570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3622..3925
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3926..4129
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4080..4100
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4131..4149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4152..4170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4180..4204
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4291..4300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4303..4329
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4345..4369
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6774..6798)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6817..7316
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6852..6875)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7249..7268
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7337..7936
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7337..7356)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7833..7853
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8003..8591
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8056..8076)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8474..8485)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8666..8899
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9018..9036)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9104..9208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9209..9277,9278..10069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9427..9446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
       61 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
      181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      661 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      721 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      781 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      841 atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
      901 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
      961 aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1021 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1081 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
     1141 tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1201 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     1261 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     1321 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     1381 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
     1441 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     1501 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     1561 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     1621 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     1681 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     1741 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     1801 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     1861 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     1921 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     1981 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     2041 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     2101 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2161 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     2221 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     2281 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     2341 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     2401 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     2461 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     2521 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     2581 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     2641 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     2701 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     2761 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     2821 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     2881 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     2941 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     3001 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     3061 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     3121 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     3181 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     3241 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     3301 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     3361 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     3421 ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     3481 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     3541 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     3601 gtttggacta gtggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     3661 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     3721 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     3781 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     3841 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     3901 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3961 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     4021 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     4081 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
     4141 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
     4201 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     4261 cagcctccgg actctagcgt ttaaacgcta gccaccatgg cgtacccata cgatgttcca
     4321 gattacgctg ctcgaggtta tcaaacaagt ttgtacaaaa aagttggcgc agccattact
     4381 gcagagatcc aggatattga agccaaaaaa gaagcacaga aggaaaaaga aattgatgaa
     4441 caggaagcga atgcctcaac atttcataga agcaggactc cactggataa agaccttatt
     4501 aatacgggga tctgtgagtc ttctggcaaa cagtgtttgc ctctggttca gctcatacaa
     4561 cagcttctta gaaacattgc ttctcagact gtagccagat tgaaagatgt tgcccgtcgg
     4621 atttcatcat gtctggactt tgagcaacac agtcgtgaaa gatctgcttc attggatttg
     4681 ttactgcgtt ttcaacgttt gcttattagt aaactttatc caggagaaag tattggtcag
     4741 acctcagata tttctagtcc agagctaatg ggtgttggtt ccttgctgaa gaagtacaca
     4801 gccctcctgt gcacgcacat tggagatata ctgcctgtgg ccgccagcat tgcttctacc
     4861 agctggcggc acttcgcgga ggtggcttac attgtggaag gggactttac tggtgttctc
     4921 cttccagaac tagtagtttc tatagtgctt ctgctcagta aaaatgctgg tctcatgcaa
     4981 gaggctggag ctgtacctct gctgggtggc ctgttggaac atctggatcg gttcaaccat
     5041 ctggcaccag gaaaggaacg ggatgatcat gaagagttag cctggcctgg cataatggag
     5101 tcatttttta caggtcagaa ctgtagaaat aatgaggaag tgacacttat acgcaaagct
     5161 gatttggaga accataataa agatggaggc ttctggactg tgattgacgg gaaggtgtat
     5221 gatataaagg acttccagac acagtcgtta acaggaaata gtattcttgc tcagtttgca
     5281 ggggaagacc cagtggtagc tttggaagct gctttgcagt ttgaagacac ccgggaatcc
     5341 atgcacgcgt tttgtgttgg ccagtatttg gagcctgacc aagaaatcgt caccatacca
     5401 gatctgggga gtctctcttc acctctgata gacacagaga ggaatctggg cctgcttctc
     5461 ggattacacg cttcgtattt ggcaatgagc acaccgctgt ctcctgtcga gattgaatgt
     5521 gccaaatggc ttcagtcatc catcttctct ggaggcctgc agaccagcca gatccactac
     5581 agctacaacg aggagaaaga cgaggaccac tgcagctccc cagggggcac acctgccagc
     5641 aaatctcgac tctgctccca cagacgggcc ctgggggacc attcccaggc atttctgcaa
     5701 gccattgcag acaacaacat tcaggatcac aacgtgaagg actttttgtg tcaaatagaa
     5761 aggtactgta ggcagtgcca tttgaccaca ccgatcatgt ttccccccga gcatcccgtg
     5821 gaagaggtcg gtcgcttgtt gttatgttgc ctcttaaaac atgaagattt aggtcatgtg
     5881 gcattatctt tagttcatgc aggtgcactt ggtattgagc aagtaaagca cagaacgttg
     5941 cctaagtcag tggtggatgt ttgtagagtt gtctaccaag caaaatgttc gctcattaag
     6001 actcatcaag aacagggccg ttcttacaag gaggtctgcg ctcctgtcat cgaacgtttg
     6061 agattcctct ttaatgaatt gagacctgct gtttgtaatg acctctctat aatgtctaag
     6121 tttaaattgt taagttcttt gccccgttgg aggaggatag ctcaaaagat aattcgagaa
     6181 cgaaggaaaa agagagttcc taagaagcca gaatctacgg atgatgaaga aaaaattgga
     6241 aacgaagaga gtgatttaga agaagcttgc attttgcctc atagtccaat aaatgtggac
     6301 aagagaccca ttgcaattaa atcacccaag gacaaatggc agccgctgtt gagtactgtt
     6361 acaggtgttc acaaatacaa gtggttgaag cagaatgtgc agggtcttta tccgcagtct
     6421 ccactcctca gtacaattgc tgaatttgcc cttaaagaag agccagtgga tgtggaaaaa
     6481 atgagaaagt gcctactaaa acagttggag agagcagagg ttcgcctgga agggatagat
     6541 acaattttaa aactggcgag caagaatttc ttacttccat ctgtgcagta tgcgatgttt
     6601 tgtggatggc aaagacttat tcctgaggga atcgatatag gggaacctct tactgattgt
     6661 ttaaaggatg ttgatttgat cccgcctttt aatcggatgc tgctggaagt cacctttggc
     6721 aagctgtacg cttgggctgt acagaacatt cgaaatgttt tgatggatgc ctgacccaac
     6781 tttcttgtac aaagtggttc gataacgaat tctaccgggt aggggaggcg cttttcccaa
     6841 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     6901 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     6961 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     7021 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     7081 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     7141 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     7201 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     7261 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     7321 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     7381 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     7441 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     7501 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     7561 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     7621 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     7681 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     7741 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     7801 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     7861 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     7921 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     7981 accccatgca tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac
     8041 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     8101 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     8161 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     8221 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     8281 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     8341 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     8401 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     8461 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     8521 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     8581 cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc
     8641 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     8701 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8761 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8821 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8881 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8941 gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca
     9001 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     9061 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     9481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     9541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     9661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9721 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    10081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10201 ctgagcgtca gaccccgt
//
