LOCUS       sequence-194438-       10818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes amino acids 2600-3600 of HERC2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 10818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p6328 pHAGE-P-CMVt-N-HA-GAW-HERC2-F4
FEATURES             Location/Qualifiers
     source          1..10818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     501..520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     754..771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1011..1032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1047..1052,1053..1070,1071..1077)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1085..1101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1090..1112
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1109..1125
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1109..1125
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1175..1194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1386..2019
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2066..2191
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2688..2921
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3106..3150
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3443..3560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3612..3915
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3916..4119
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4070..4090
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4121..4139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4142..4160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4170..4194
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4281..4290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4293..4319
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4335..4359
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7364..7388)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7407..7906
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7442..7465)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7839..7858
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7927..8526
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7927..7946)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8423..8443
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8593..9181
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8646..8666)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9064..9075)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9256..9489
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9608..9626)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9694..9798
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9799..9867,9868..10659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10017..10036)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
       61 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      781 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
      841 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      901 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
      961 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     1021 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     1081 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     1141 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1201 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     1261 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     1321 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     1381 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     1441 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     1501 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     1561 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     1621 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     1681 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     1741 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     1801 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     1861 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1921 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1981 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     2041 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     2101 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     2161 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     2221 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     2281 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     2341 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     2401 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     2461 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     2521 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     2581 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     2641 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     2701 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     2761 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     2821 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2881 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2941 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     3001 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     3061 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     3121 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     3181 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     3241 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     3301 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     3361 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattcac
     3421 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     3481 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     3541 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggacta
     3601 gtggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3661 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3721 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3781 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3841 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     3901 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     3961 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4021 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     4081 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat
     4141 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
     4201 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
     4261 actctagcgt ttaaacgcta gccaccatgg cgtacccata cgatgttcca gattacgctg
     4321 ctcgaggtta tcaaacaagt ttgtacaaaa aagttggcga tggattgcat gatctcaatg
     4381 tgcagtgtga ctggcagcag aaagggggca cctactgggt taggtacatt catgtggaac
     4441 ttataggcta tcctccacca agttcttctt ctcacatcaa gattggtgat aaagtgcggg
     4501 tcaaagcctc tgtcaccaca ccaaaataca aatggggatc tgtgactcat cagagtgtgg
     4561 gggttgtgaa agctttcagt gccaatggaa aagatatcat tgtcgacttt ccccagcagt
     4621 ctcactggac tgggttgcta tcagaaatgg agttggtacc cagtattcat cctggggtta
     4681 cgtgtgatgg atgtcagatg tttcctatca atggatccag attcaaatgc agaaactgtg
     4741 atgactttga tttttgtgaa acgtgtttca agaccaaaaa acacaatacc aggcatacat
     4801 ttggcagaat aaatgaacca ggtcagtctg cggtattttg tggccgttct ggaaaacagc
     4861 tgaagcgttg ccacagcagc cagccaggca tgctgctgga cagctggtcc cgcatggtga
     4921 agagcctgaa tgtgtcgtcc tccgtgaacc aggcatcccg tctcattgac ggcagcgagc
     4981 cctgctggca gtcatcgggg tcgcaaggaa agcactggat tcgtttggag attttcccag
     5041 atgttcttgt tcatagatta aaaatgatcg tagatcctgc tgacagtagc tacatgccgt
     5101 ccctggttgt agtgtcaggt ggaaattccc tgaataacct tattgaacta aagacaatca
     5161 atattaaccc ttctgacacc acagtgcccc ttctgaatga ctgcacagag tatcacaggt
     5221 atattgaaat tgctataaag cagtgcagga gctcaggaat cgattgtaaa atccatggtc
     5281 tcatcctgct gggacggatc cgtgcagaag aggaagattt ggctgcagtt cctttcttag
     5341 cttcggataa tgaagaggag gaggatgaga aaggcaacag cggaagcctc attagaaaga
     5401 aggctgctgg gctggaatca gcagctacga taagaaccaa ggtgtttgtg tggggcctga
     5461 atgacaagga ccagctgggc gggctgaaag gctccaagat aaaggttcct tcgttctctg
     5521 agacactgtc agctttgaat gtggtacagg tggctggtgg atctaaaagt ttgtttgcag
     5581 tgactgtgga agggaaggtg tatgcctgtg gagaagccac gaatggccgg ctggggctgg
     5641 gcatttccag cgggacggtg cccatcccac ggcagatcac agctctcagc agctacgtgg
     5701 tcaagaaggt ggctgttcac tcaggtggcc ggcacgcgac ggctttaact gtcgatggaa
     5761 aagtgttttc gtggggcgaa ggtgacgatg gaaaacttgg acacttcagc agaatgaact
     5821 gtgacaaacc aaggctgatc gaggccctga aaaccaagcg tatccgggat atcgcctgtg
     5881 ggagctcgca cagcgcagcc ctcacatcca gcggagaact gtacacctgg ggcctcggcg
     5941 agtacggccg gctgggacat ggggataata cgacacagct aaagcccaaa atggtgaaag
     6001 tccttctcgg tcacagagta atccaggttg catgtgggag tagagacgcg cagaccctgg
     6061 ctctgaccga tgaaggtttg gtattttcct ggggtgatgg tgactttgga aaactgggcc
     6121 ggggcggaag tgaaggctgt aacattcccc agaacattga gagactaaat ggacaggggg
     6181 tgtgccagat tgagtgtgga gctcagttct ccctggcgct caccaagtct ggagtggtgt
     6241 ggacatgggg aaagggggat tacttcagat tgggccacgg ctctgacgtg cacgtgcgga
     6301 aaccacaggt ggtggaaggg ctgagaggga agaagatcgt gcatgtggct gtcggggccc
     6361 tgcactgcct ggcggtcacg gactcggggc aggtgtatgc ttggggtgac aacgaccacg
     6421 gccagcaggg caatggcacg accacggtta acaggaagcc cacactcgtg caaggcttag
     6481 aaggccagaa gatcacacgc gtggcttgtg ggtcgtccca cagtgtggcg tggacaactg
     6541 tggatgtggc cacgccctct gtccacgagc ccgtcctctt ccagactgca agagaccctt
     6601 taggtgcttc ctatttaggc gtgccttcag atgctgattc ttctgctgcc agtaataaaa
     6661 taagtggtgc aagtaattct aagccaaatc gcccttctct tgccaagatt ctcttgtcat
     6721 tggatggaaa tctggccaaa cagcaggcct tatcgcatat tcttacagca ttgcaaatca
     6781 tgtatgccag agatgctgtt gttggggccc tgatgccggc cgccatgatc gccccggtgg
     6841 agtgcccctc gttctcctcg gcggcccctt ccgacgcatc tgcgatggct agtcccatga
     6901 atggagaaga atgcatgctg gctgttgata tcgaagacag actgagtcca aatccatggc
     6961 aagaaaagag agagattgtt tcctctgagg acgcagtgac cccctctgca gtgactccgt
     7021 cggccccctc agcctccgct cggcctttta tcccagtgac ggatgacctg ggagccgcaa
     7081 gcatcattgc agaaaccatg accaaaacca aagaggatgt tgaaagccaa aataaagcag
     7141 caggtccgga gcctcaggcc ttggatgagt tcaccagtct gctgattgcg gatgacactc
     7201 gtgtggtggt agacctgctc aagctgtcag tgtgcagccg ggccggggac aggggcaggg
     7261 atgtgctctc cgcggtgctt tccggcatgg ggaccgccta cccacaggtg gcagatatgc
     7321 tgttggagct ctgtgtcacc gagttggagg atgtggccac atgacccaac tttcttgtac
     7381 aaagtggttc gataacgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     7441 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     7501 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     7561 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     7621 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     7681 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     7741 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     7801 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     7861 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag
     7921 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     7981 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     8041 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     8101 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     8161 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     8221 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     8281 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     8341 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     8401 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     8461 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     8521 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     8581 tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8641 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8701 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8761 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8821 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8881 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8941 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     9001 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     9061 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9121 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9181 cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9241 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     9301 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9481 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     9541 gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga
     9601 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     9661 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9721 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     9781 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     9841 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     9901 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9961 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
    10021 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
    10081 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
    10141 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
    10201 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    10261 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    10321 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
    10381 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    10441 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    10501 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    10561 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    10621 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    10681 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    10741 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    10801 gaccccgtag aaaagatc
//
