LOCUS       sequence-194439-        8893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for phycocyanobilin (PCB) synthesis and shRNA for 
            human BVRA gene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Uda Y, Goto Y, Oda S, Kohchi T, Matsuda M, Aoki K
  TITLE     Efficient synthesis of phycocyanobilin in mammalian cells for 
            optogenetic control of cell signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Oct 24. pii: 201707190. doi: 
            10.1073/pnas.1707190114.
  PUBMED    29078307
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Oct 24. pii: 201707190. doi: 
            10.1073/pnas.1707190114."
COMMENT     Sequence Label: pCAGGS-PHFF-sh-hBVRA
FEATURES             Location/Qualifiers
     source          1..8893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(89..303)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(149..168)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     enhancer        312..691
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        693..968
                     /label=chicken beta-actin promoter
     intron          969..1981
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1905..1927
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1989..2008
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2039..2113
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      2560..2569
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2849..2872
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2882..2938
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2942..3016
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3047..3073
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3797..3853
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3854..3928
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3959..3988
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4289..4345
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4346..4420
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     complement(5758..5778)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5758..5776)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5797..5813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5915..5934)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5980..6035
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6034..6053)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6396..6412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6420..6436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6444..6450,6451..6468,6469..6474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6489..6510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6569..6764
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6615..6750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6770..6904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6807..6826)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6861..6880
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6972..6989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7143..7731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7223..7242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7902..8693,8694..8762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8525..8544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8763..8867)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggta tcgataagct taaaaaagcg aaaggaagat cagtatatga tctcttgaat
       61 catatactga tcttcctttc gcagatctgt ggtctcatac agaacttata agattcccaa
      121 atccaaagac atttcacgtt tatggtgatt tcccagaaca catagcgaca tgcaaatatt
      181 gcagggcgcc actcccctgt ccctcacagc catcttcctg ccagggcgca cgcgcgctgg
      241 gtgttcccgc ctagtgacac tgggcccgcg attccttgga gcgggttgat gacgtcagcg
      301 ttcgaattct cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      361 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      421 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      481 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      541 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      601 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      661 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
      721 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
      781 ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcgggggc
      841 gagggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
      901 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
      961 ggcgggcggg agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc
     1021 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     1081 ctcctccggg ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc
     1141 gtgaaagcct taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt
     1201 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg
     1261 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc
     1321 cgggggcggt gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt
     1381 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct
     1441 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg
     1501 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1561 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1621 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1681 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1741 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1801 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1861 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1921 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1981 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcaccat
     2041 gtccgtcctg actccactgc tgctgagggg cctgactggg tccgcacgcc gcctgcctgt
     2101 cccaagagcc aaaatccata gcctgggcgg gtctggaggc atgagtctga ggcagcacca
     2161 gcatcctctg atccagcgcc tggccgatcg aatcgaggca atttggcagg ccttctttcc
     2221 cctggcacct tacgccctgc cagaagacct gggctatgtg gagggaaaac tggagggcga
     2281 acgcctgaca atcgagaacc actgctatca ggccccccct tttcggaagc tgcatctgga
     2341 actggctaga gtgggcgagt ctctggacat tctgcactgc gtcatgtttc ccgagcctag
     2401 atacgatctg cccatgttcg gatgtgacct ggtgggggga cgcggacaga tcagtgccgc
     2461 tattgtggat ctgtcaccag tcactggaca gctgccagca gcatacacct gcgctctgaa
     2521 tgcactgcct aagctgacct ttagacagcc aagggagctg ccaccatggg gacatatctt
     2581 ctctcccttt tgtatcttca ttagacctca gggcgaagcc gaggaacagc agttcctgga
     2641 taggattggg gagtatctga ctctgcactg ccagctgagc cagcaggcag tgcctaccga
     2701 ccatccacag gccgtcatcg ctggacagcg gcagtactgt cagcagcagc agcagaacga
     2761 taaaactcgg agagtgctgg aaaaggcttt tggcgtccca tgggcagaga gatacatgac
     2821 cacagtgctg ttcgatgtgc ctccagtgga ctataaggac gatgacgata aaggcagcgg
     2881 ggccaccaac ttctccctgc tgaagcaggc tggcgacgtg gaggaaaatc caggacccgg
     2941 catgtcagtc ctgacacctc tgctgctgcg aggactgact ggaagcgcca ggcgcctgcc
     3001 tgtgccacga gcaaaaatcc actcactggg cgggagcgga ggcatgtacc cctatgatgt
     3061 ccctgactac gccatgacta ccagcctggc tacaaagctg agggaaggca ctaagaaagc
     3121 tcataccatg gcagagaacg tggggtttgt ccggtgtttc ctgaagggaa ctgtggagaa
     3181 aagctcctat agaaagctgg tggcctctct gtaccacgtc tatagtgcta tggagcagga
     3241 aatggagcgc ctgaaagacc atccaatcgt gggcaagatc tactttcccg aactgaacag
     3301 gaagtctagt ctggagcgcg atctgacata ctatttcggg tccaattgga gggaggaaat
     3361 tcccccttct cccgcaactc aggcctacgt ggctcgcatc cacgaagtcg caaacaccgc
     3421 ccctgagctg ctggtggccc atagttacac acggtatctg ggagacctgt cagggggaca
     3481 gatcctgaag ggcattgctg aacgggcaat gaatctgcag gatggggagg gaaccgcctt
     3541 ctacagattc gaaagtatct cagacgagaa ggccttcaaa cagctgtatc gacagcggct
     3601 ggatgaactg cccgtggacg aggctacagc agatagaatt gtggacgagg ctaacgctgc
     3661 atttggcatg aatatgaaga tcttccagga actggagggc aacctgatcc gggccattgg
     3721 gcagctgctg tttaataccc tgacacgacg gaaacagaga ggcagcaccg agctggccac
     3781 agctgatggc tcaggggcaa ccaacttcag cctgctgaag caggccggag acgtggaaga
     3841 gaatccagga cctatgtccg tcctgacccc actgctgctg aggggactga caggatctgc
     3901 aagaaggctg ccagtgccca gagccaaaat tcactccctg ggaggctctg gaggcatgga
     3961 gcagaagctg atcagcgagg aagatctgat ggcctcctat acagtgaaac tgatcactcc
     4021 cgacggcgaa tcaagcattg agtgctccga cgatacctac atcctggatg cagctgagga
     4081 agcagggctg gacctgcctt attcttgtag ggcaggagcc tgcagtactt gtgcaggcaa
     4141 gattaccgcc gggagcgtgg atcagagtga ccagtcattt ctggacgatg accagatcga
     4201 agctggctac gtgctgacat gcgtcgcata tcccacttcc gattgtacta ttgagaccca
     4261 caaagaggaa gacctgtacg gatccggcgc tacaaacttc tctctgctga agcaggcagg
     4321 ggacgtggag gaaaatcctg gaccaatgag tgtcctgact cccctgctgc tgagaggact
     4381 gaccggaagc gcccgccgac tgcccgtgcc tagagctaag atccatagtc tggggggatc
     4441 aggcgggatg tacagcccag gatatgtggc caccagcagc cggcagtccg atgcaggaaa
     4501 caggctgttc gtgtacgagg tcatcggcct gagccagtcc actatgaccg atgggctgga
     4561 ctatcccatt cggagatctg gaagtacatt catcactgtg cctctgaaac gaatgaatca
     4621 ggagatgagg cgcatcaccc ggatgggagg caaaatcgtg tcaattaagc ctctggaagg
     4681 cgacagccca ctgccacaca cagagggaat tgccaagcca tcacagagcg aagggtccgg
     4741 atctgaggct gtggcaaacc ctgctccaga aagcaacaag actatgacaa ctacccctaa
     4801 ggagaagaaa gccgatgaca tcccagtgaa catctacagg ccaaaaaccc cctatattgg
     4861 gaaggtgctg gaaaattacc cactggtccg cgagggagct atcggaaccg tgcagcacct
     4921 gacatttgat ctgtctgccg gcgacctgcg gtatctggaa gggcagagta tcggaatcat
     4981 tccacccgga gaggatgaca agggcaaacc tcacaaactg aggctgtaca gcattgcctc
     5041 cacacgccat ggggatttcg gagatgacaa gactgtgagc ctgtgcgtca gacagctgga
     5101 ataccagaac gaagcaggag agaccgtgca gggagtctgc tccacatacc tgtgcaatat
     5161 caaagagggc gatgacatcg ccattaccgg cccagtgggg aaggaaatgc tgctgcctcc
     5221 agatgaggac gcaaacattg tcatgctggc cacaggcact gggatcgccc cctttcgggc
     5281 tttcctgtgg agaatgttca aagaacagca cgaggactac aagtttaaag gcctggcctg
     5341 gctgatcttc gggattccaa agtctgagaa tatcctgtat aaagatgacc tggaaaagat
     5401 ggcagccgag tttcccgata acttccgcct gacatacgct attagccgag aacagcagaa
     5461 tgcagagggg ggacgaatgt atatccagca ccgggtggcc gagaacgctg aggaactgtg
     5521 gaacctgatg cagaatccaa aaacccatac atacatgtgc ggactgaagg gcatggaacc
     5581 cgggatcgac gaggccttta ccgccctggc tgaacagaat ggaaaggagt ggacaacttt
     5641 ccagagagag atgaagaaag aacatagatg gcacgtcgag acctacatgg cttcaatgac
     5701 aggaggacag cagatggggt agcggccgcc accgcggtgg agctccagct tttgttccct
     5761 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5821 ttgttatccg ctaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
     5881 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
     5941 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     6001 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     6061 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     6121 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     6181 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     6241 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     6301 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
     6361 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6421 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6481 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6541 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
     6601 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6661 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6721 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
     6781 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6841 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6901 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6961 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7021 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7861 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7921 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7981 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8041 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8101 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8161 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8221 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8281 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8341 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8401 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8461 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8521 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8581 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8641 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8701 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8761 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8881 aaagtgccac ctg
//
