LOCUS       sequence-194440-       10666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes amino acids 3550-4500 of HERC2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 10666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p6329 pHAGE-P-CMVt-N-HA-GAW-HERC2-F5
FEATURES             Location/Qualifiers
     source          1..10666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      20..608
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     509..528
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     762..779
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1019..1040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1055..1060,1061..1078,1079..1085)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1093..1109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1098..1120
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1117..1133
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1117..1133
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1183..1202
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1394..2027
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2074..2199
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2696..2929
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3114..3158
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3451..3568
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3620..3923
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3924..4127
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4078..4098
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4129..4147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4150..4168
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4178..4202
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4289..4298
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4301..4327
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4343..4367
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7222..7246)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7265..7762
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7300..7323)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7695..7714
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7783..8382
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7783..7802)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8279..8299
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8449..9037
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8502..8522)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8920..8931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9112..9345
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9464..9482)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9550..9654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9655..9723,9724..10515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9873..9892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
       61 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      121 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      181 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      241 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      301 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      361 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      421 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      481 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      541 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      601 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      661 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      721 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      781 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      841 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      901 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      961 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     1021 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     1081 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     1141 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1201 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     1261 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     1321 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     1381 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     1441 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     1501 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     1561 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     1621 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     1681 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     1741 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     1801 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     1861 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1921 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1981 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2041 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     2101 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     2161 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     2221 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     2281 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     2341 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     2401 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     2461 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     2521 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     2581 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     2641 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     2701 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     2761 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     2821 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     2881 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     2941 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     3001 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     3061 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     3121 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     3181 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     3241 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     3301 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     3361 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     3421 gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     3481 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     3541 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     3601 ttggactagt ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3661 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3721 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3781 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3841 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3901 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3961 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4021 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4081 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg
     4141 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
     4201 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     4261 gcctccggac tctagcgttt aaacgctagc caccatggcg tacccatacg atgttccaga
     4321 ttacgctgct cgaggttatc aaacaagttt gtacaaaaaa gttggcgtag acctgctcaa
     4381 gctgtcagtg tgcagccggg ccggggacag gggcagggat gtgctctccg cggtgctttc
     4441 cggcatgggg accgcctacc cacaggtggc agatatgctg ttggagctct gtgtcaccga
     4501 gttggaggat gtggccacag actcgcagag cggccgcctc tcttctcagc ctgtggtggt
     4561 ggagagtagc cacccttaca ccgacgacac ctccaccagt ggcacagtga agataccagg
     4621 tgcagaagga ctcagggtag aatttgaccg gcagtgctcc acagagaggc gccacgaccc
     4681 tctcacagtc atggacggcg tcaacaggat cgtctccgtg cggtcaggcc gagagtggtc
     4741 cgactggtcc agcgagctgc gcatcccagg ggatgagtta aagtggaagt tcatcagcga
     4801 tgggtctgtg aatggctggg gctggcgctt caccgtctat cccatcatgc cagctgctgg
     4861 ccctaaagaa ctcctctctg accgctgcgt cctctcctgt ccatccatgg acttggtgac
     4921 gtgtctgtta gacttccgac tcaaccttgc ctctaacaga agcatcgtcc ctcgccttgc
     4981 ggcctcgctg gcagcttgtg cacagctgag tgccctagct gccagtcaca gaatgtgggc
     5041 ccttcagaga ctgaggaagc tgcttacaac tgaatttggg cagtcaatta acataaatag
     5101 gctgcttgga gaaaatgatg gggaaacaag agctttgagt tttacaggta gtgctcttgc
     5161 tgctttggtg aaaggtcttc cagaagcttt gcaaaggcag tttgaatatg aagatcctat
     5221 tgtgaggggt ggcaaacagc tgctccacag cccattcttt aaggtactgg tagctcttgc
     5281 ttgtgacctg gagctggaca ctctgccttg ctgtgccgag acgcacaagt gggcctggtt
     5341 ccggaggtac tgcatggcct cccgtgttgc tgtggccctt gacaaaagaa caccgttgcc
     5401 ccgtctgttt cttgatgagg tggctaagaa aattcgtgaa ttaatggcag acagcgaaaa
     5461 catggatgtt ctgcatgaga gccatgacat ttttaaaaga gagcaagacg aacaacttgt
     5521 gcagtggatg aacaggcgac cagatgactg gactctctct gctggtggca gtggaacaat
     5581 ttatggatgg ggacataatc acaggggcca gctcgggggc attgaaggcg caaaagtcaa
     5641 agttcccact ccctgtgaag cccttgcaac tctcagaccc gtgcaattaa tcggagggga
     5701 acagaccctc tttgctgtga cggctgatgg gaagctgtat gccactgggt atggtgcagg
     5761 tggcagacta ggcattggag ggacagagtc ggtgtccacc ccaacattgc ttgaatccat
     5821 tcagcatgtg tttattaaga aagtagctgt gaactctgga ggaaagcact gccttgccct
     5881 gtcttcagaa ggagaagttt actcttgggg tgaggcagaa gatgggaagt tggggcatgg
     5941 caacagaagt ccgtgtgacc gccctcgtgt catcgagtct ctgagaggaa ttgaagtggt
     6001 cgatgttgct gctggcggag cccacagcgc ctgtgtcaca gcagccgggg acctctacac
     6061 atggggcaaa ggccgctacg gccggctggg gcacagcgac agtgaggacc agctgaagcc
     6121 gaagctggtg gaggcgctgc agggccaccg tgtggttgac atcgcctgtg gcagtggaga
     6181 tgcccagacc ctctgcctca cagatgacga cactgtctgg tcctgggggg acggggacta
     6241 cggcaagctc ggccggggag gcagcgatgg ctgtaaagtg cctatgaaga ttgattctct
     6301 tactggtctt ggagtagtta aagtggaatg cggatcccag ttttctgttg cccttaccaa
     6361 atctggagct gtttatacct ggggcaaagg cgattatcac aggttgggcc atggatcaga
     6421 tgaccatgtt cgaaggcctc ggcaggtcca agggttgcag gggaagaaag tcatcgccat
     6481 cgccactggc tccctgcact gtgtgtgctg cacagaggat ggtgaggttt atacatgggg
     6541 cgacaatgat gagggacaac tgggagacgg aaccaccaat gccatccaga ggcctcggtt
     6601 ggtagctgcc cttcagggta agaaggtcaa ccgtgtggcc tgtggctcag cacataccct
     6661 cgcctggtcg accagcaagc ccgccagtgc tggcaaactc cctgcacagg tccccatgga
     6721 gtacaatcac ctgcaggaga tccccatcat tgcgctgagg aaccgtctgc tgctgctgca
     6781 ccacctctcc gagctcttct gcccctgcat ccccatgttc gacctggaag gctcgctcga
     6841 cgaaactgga ctcgggcctt ctgttgggtt cgacactctc cgaggaattc tgatatccca
     6901 gggaaaggag gcggctttcc ggaaagtagt acaagcaact atggtacgcg atcgtcagca
     6961 tggccccgtc gtggagctga accgcatcca ggtcaaacga tcaaggagca aaggcgggct
     7021 ggccggcccc gacggcacca agtctgtctt tgggcagatg tgtgctaaga tgagctcgtt
     7081 tggtcccgac agcctcctcc ttcctcaccg tgtctggaaa gtcaagtttg tgggtgaatc
     7141 tgtggatgac tgtgggggcg gctacagcga gtccatagct gagatctgtg aggagctgca
     7201 gaacggactc acgcccctgt gacccaactt tcttgtacaa agtggttcga taacgaattc
     7261 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     7321 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     7381 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     7441 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     7501 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     7561 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     7621 aggggtgggt ccgggggggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     7681 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     7741 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
     7801 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     7861 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     7921 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     7981 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     8041 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     8101 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     8161 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     8221 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     8281 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     8341 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     8401 cagcgcccga ccgaaaggag cgcacgaccc catgcatcga tagatcctaa tcaacctctg
     8461 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8521 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8581 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8641 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8701 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8761 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8821 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8881 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8941 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     9001 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
     9061 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     9121 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     9181 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9241 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9301 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     9361 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
     9421 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
     9481 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     9541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9781 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9841 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     9901 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9961 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    10021 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10081 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10141 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    10201 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    10261 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    10321 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    10381 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    10441 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    10501 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10561 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtag
//
