LOCUS       sequence-194441-        9057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes amino acids 4421-4834 of HERC2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 9057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p6330 pHAGE-P-CMVt-N-HA-GAW-HERC2-F6
FEATURES             Location/Qualifiers
     source          1..9057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     514..533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     767..784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1024..1045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1060..1065,1066..1083,1084..1090)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1098..1114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1103..1125
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1122..1138
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1122..1138
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1188..1207
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1399..2032
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2079..2204
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2701..2934
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3119..3163
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3456..3573
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3625..3928
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3929..4132
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4083..4103
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4134..4152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4155..4173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4183..4207
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4294..4303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4306..4332
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4348..4372
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5616..5640)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5659..6158
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5694..5717)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6091..6110
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6179..6778
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6179..6198)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6675..6695
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6845..7433
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6898..6918)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7316..7327)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7508..7741
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7860..7878)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7946..8050
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8051..8119,8120..8911)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8269..8288)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
       61 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      781 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      841 ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      901 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      961 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     1021 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     1081 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
     1141 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1201 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     1261 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     1321 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     1381 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     1441 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     1501 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     1561 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     1621 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     1681 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     1741 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     1801 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     1861 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     1921 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     1981 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     2041 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     2101 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     2161 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     2221 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     2281 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     2341 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     2401 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     2461 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     2521 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     2581 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     2641 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     2701 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     2761 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     2821 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     2881 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     2941 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     3001 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     3061 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     3121 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     3181 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     3241 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     3301 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     3361 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     3421 tcgccgaatt cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     3481 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     3541 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     3601 ccagtttgga ctagtggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     3661 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3721 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3781 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3841 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3901 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3961 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     4021 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     4081 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat
     4141 cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg
     4201 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
     4261 atccagcctc cggactctag cgtttaaacg ctagccacca tggcgtaccc atacgatgtt
     4321 ccagattacg ctgctcgagg ttatcaaaca agtttgtaca aaaaagttgg caaccgcatc
     4381 caggtcaaac gatcaaggag caaaggcggg ctggccggcc ccgacggcac caagtctgtc
     4441 tttgggcaga tgtgtgctaa gatgagctcg tttggtcccg acagcctcct ccttcctcac
     4501 cgtgtctgga aagtcaagtt tgtgggtgaa tctgtggatg actgtggggg cggctacagc
     4561 gagtccatag ctgagatctg tgaggagctg cagaacggac tcacgcccct gctgatcgtg
     4621 acacccaacg ggagggatga gtctggggcc aaccgagact gctacctgct cagcccggcc
     4681 gccagagcac ccgtgcacag cagcatgttc cgcttcctgg gtgtgttgct gggcattgcc
     4741 atccgaaccg ggagtcccct gagcctcaac cttgccgagc ctgtctggaa gcagctggct
     4801 gggatgagcc tcaccatcgc ggacctcagt gaggttgata aggattttat tcctggactc
     4861 atgtacatcc gagacaatga agccacctca gaggagtttg aagccatgag cctgcccttc
     4921 acagtgccaa gtgccagtgg ccaggacatt cagttgagct ccaagcacac acacatcacc
     4981 ctggacaacc gcgcggagta cgtgcggctg gcgataaact atagactcca tgaatttgat
     5041 gagcaggtgg ctgctgttcg ggaaggaatg gcccgcgttg tgcctgttcc cctcctctct
     5101 ctgttcaccg gctacgaact ggagacgatg gtgtgtggca gccctgacat cccgctgcac
     5161 cttctcaagt cggtggccac ctataaaggc atcgagcctt ccgcatcgct gatccagtgg
     5221 ttctgggagg tgatggagtc cttctccaac acagagcgct ctcttttcct tcgcttcgtc
     5281 tggggccgga cgaggctgcc caggaccatc gccgacttcc ggggccgaga cttcgtcatc
     5341 caggtgttgg ataaatacaa ccctccagac cacttcctcc ctgagtccta cacctgtttc
     5401 ttcttgctga agctgcccag gtattcctgc aagcaggtgc tggaggagaa gctcaagtac
     5461 gccatccact tctgcaagtc catagacaca gatgactacg ctcgcatcgc acttacagga
     5521 gagccagccg ccgacgacag cagcgacgat tcagataacg aggatgtcga ctcctttgct
     5581 tcggactcta cacaagatta tttaacagga cactgaccca actttcttgt acaaagtggt
     5641 tcgataacga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     5701 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     5761 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     5821 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     5881 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     5941 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     6001 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     6061 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     6121 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     6181 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6241 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6301 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6361 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6421 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6481 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6541 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6601 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6661 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6721 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     6781 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgataga
     6841 tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6901 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6961 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7021 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7081 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7141 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7201 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     7261 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7321 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7381 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat
     7441 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7501 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     7561 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7621 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7681 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7741 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7801 gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg
     7861 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7921 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7981 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8041 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8101 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8161 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8221 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8281 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8341 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8401 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8461 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8521 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8581 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8641 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8701 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8761 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8821 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8881 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8941 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9001 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccc
//
