LOCUS       sequence-194443-        8891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for phycocyanobilin (PCB) synthesis and shRNA for 
            mouse BVRA gene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Uda Y, Goto Y, Oda S, Kohchi T, Matsuda M, Aoki K
  TITLE     Efficient synthesis of phycocyanobilin in mammalian cells for 
            optogenetic control of cell signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Oct 24. pii: 201707190. doi: 
            10.1073/pnas.1707190114.
  PUBMED    29078307
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Oct 24. pii: 201707190. doi: 
            10.1073/pnas.1707190114."
COMMENT     Sequence Label: pCAGGS-PHFF-sh-mBVRA
FEATURES             Location/Qualifiers
     source          1..8891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(87..301)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(147..166)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     enhancer        310..689
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        691..966
                     /label=chicken beta-actin promoter
     intron          967..1979
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1903..1925
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1987..2006
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2037..2111
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      2558..2567
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2847..2870
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2880..2936
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2940..3014
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3045..3071
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3795..3851
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3852..3926
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3957..3986
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4287..4343
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4344..4418
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     complement(5756..5776)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5756..5774)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5795..5811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5913..5932)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5978..6033
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6032..6051)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6394..6410)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6418..6434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6442..6448,6449..6466,6467..6472))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6487..6508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6567..6762
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6613..6748
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6768..6902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6805..6824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6859..6878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6970..6987)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7141..7729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7221..7240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7900..8691,8692..8760))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8523..8542
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8761..8865)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggta tcgataagct taaaaagcca aatgtaggag tcaataatct cttgaattat
       61 tgactcctac atttggcggg agatctgtgg tctcatacag aacttataag attcccaaat
      121 ccaaagacat ttcacgttta tggtgatttc ccagaacaca tagcgacatg caaatattgc
      181 agggcgccac tcccctgtcc ctcacagcca tcttcctgcc agggcgcacg cgcgctgggt
      241 gttcccgcct agtgacactg ggcccgcgat tccttggagc gggttgatga cgtcagcgtt
      301 cgaattctcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      361 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      421 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      481 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      541 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      601 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      661 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
      721 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
      781 gtgcagcgat gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcgggggcga
      841 gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
      901 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
      961 cgggcgggag tcgctgcgtt gccttcgccc cgtgccccgc tccgcgccgc ctcgcgccgc
     1021 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     1081 cctccgggct gtaattagcg cttggtttaa tgacggctcg tttcttttct gtggctgcgt
     1141 gaaagcctta aagggctccg ggagggccct ttgtgcgggg gggagcggct cggggggtgc
     1201 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggcccg cgctgcccgg cggctgtgag
     1261 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcgtgt gcgcgagggg agcgcggccg
     1321 ggggcggtgc cccgcggtgc gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1381 gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc gggctgtaac ccccccctgc
     1441 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtgcggggc
     1501 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1561 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1621 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1681 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1741 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1801 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1861 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1921 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1981 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcaccatgt
     2041 ccgtcctgac tccactgctg ctgaggggcc tgactgggtc cgcacgccgc ctgcctgtcc
     2101 caagagccaa aatccatagc ctgggcgggt ctggaggcat gagtctgagg cagcaccagc
     2161 atcctctgat ccagcgcctg gccgatcgaa tcgaggcaat ttggcaggcc ttctttcccc
     2221 tggcacctta cgccctgcca gaagacctgg gctatgtgga gggaaaactg gagggcgaac
     2281 gcctgacaat cgagaaccac tgctatcagg cccccccttt tcggaagctg catctggaac
     2341 tggctagagt gggcgagtct ctggacattc tgcactgcgt catgtttccc gagcctagat
     2401 acgatctgcc catgttcgga tgtgacctgg tggggggacg cggacagatc agtgccgcta
     2461 ttgtggatct gtcaccagtc actggacagc tgccagcagc atacacctgc gctctgaatg
     2521 cactgcctaa gctgaccttt agacagccaa gggagctgcc accatgggga catatcttct
     2581 ctcccttttg tatcttcatt agacctcagg gcgaagccga ggaacagcag ttcctggata
     2641 ggattgggga gtatctgact ctgcactgcc agctgagcca gcaggcagtg cctaccgacc
     2701 atccacaggc cgtcatcgct ggacagcggc agtactgtca gcagcagcag cagaacgata
     2761 aaactcggag agtgctggaa aaggcttttg gcgtcccatg ggcagagaga tacatgacca
     2821 cagtgctgtt cgatgtgcct ccagtggact ataaggacga tgacgataaa ggcagcgggg
     2881 ccaccaactt ctccctgctg aagcaggctg gcgacgtgga ggaaaatcca ggacccggca
     2941 tgtcagtcct gacacctctg ctgctgcgag gactgactgg aagcgccagg cgcctgcctg
     3001 tgccacgagc aaaaatccac tcactgggcg ggagcggagg catgtacccc tatgatgtcc
     3061 ctgactacgc catgactacc agcctggcta caaagctgag ggaaggcact aagaaagctc
     3121 ataccatggc agagaacgtg gggtttgtcc ggtgtttcct gaagggaact gtggagaaaa
     3181 gctcctatag aaagctggtg gcctctctgt accacgtcta tagtgctatg gagcaggaaa
     3241 tggagcgcct gaaagaccat ccaatcgtgg gcaagatcta ctttcccgaa ctgaacagga
     3301 agtctagtct ggagcgcgat ctgacatact atttcgggtc caattggagg gaggaaattc
     3361 ccccttctcc cgcaactcag gcctacgtgg ctcgcatcca cgaagtcgca aacaccgccc
     3421 ctgagctgct ggtggcccat agttacacac ggtatctggg agacctgtca gggggacaga
     3481 tcctgaaggg cattgctgaa cgggcaatga atctgcagga tggggaggga accgccttct
     3541 acagattcga aagtatctca gacgagaagg ccttcaaaca gctgtatcga cagcggctgg
     3601 atgaactgcc cgtggacgag gctacagcag atagaattgt ggacgaggct aacgctgcat
     3661 ttggcatgaa tatgaagatc ttccaggaac tggagggcaa cctgatccgg gccattgggc
     3721 agctgctgtt taataccctg acacgacgga aacagagagg cagcaccgag ctggccacag
     3781 ctgatggctc aggggcaacc aacttcagcc tgctgaagca ggccggagac gtggaagaga
     3841 atccaggacc tatgtccgtc ctgaccccac tgctgctgag gggactgaca ggatctgcaa
     3901 gaaggctgcc agtgcccaga gccaaaattc actccctggg aggctctgga ggcatggagc
     3961 agaagctgat cagcgaggaa gatctgatgg cctcctatac agtgaaactg atcactcccg
     4021 acggcgaatc aagcattgag tgctccgacg atacctacat cctggatgca gctgaggaag
     4081 cagggctgga cctgccttat tcttgtaggg caggagcctg cagtacttgt gcaggcaaga
     4141 ttaccgccgg gagcgtggat cagagtgacc agtcatttct ggacgatgac cagatcgaag
     4201 ctggctacgt gctgacatgc gtcgcatatc ccacttccga ttgtactatt gagacccaca
     4261 aagaggaaga cctgtacgga tccggcgcta caaacttctc tctgctgaag caggcagggg
     4321 acgtggagga aaatcctgga ccaatgagtg tcctgactcc cctgctgctg agaggactga
     4381 ccggaagcgc ccgccgactg cccgtgccta gagctaagat ccatagtctg gggggatcag
     4441 gcgggatgta cagcccagga tatgtggcca ccagcagccg gcagtccgat gcaggaaaca
     4501 ggctgttcgt gtacgaggtc atcggcctga gccagtccac tatgaccgat gggctggact
     4561 atcccattcg gagatctgga agtacattca tcactgtgcc tctgaaacga atgaatcagg
     4621 agatgaggcg catcacccgg atgggaggca aaatcgtgtc aattaagcct ctggaaggcg
     4681 acagcccact gccacacaca gagggaattg ccaagccatc acagagcgaa gggtccggat
     4741 ctgaggctgt ggcaaaccct gctccagaaa gcaacaagac tatgacaact acccctaagg
     4801 agaagaaagc cgatgacatc ccagtgaaca tctacaggcc aaaaaccccc tatattggga
     4861 aggtgctgga aaattaccca ctggtccgcg agggagctat cggaaccgtg cagcacctga
     4921 catttgatct gtctgccggc gacctgcggt atctggaagg gcagagtatc ggaatcattc
     4981 cacccggaga ggatgacaag ggcaaacctc acaaactgag gctgtacagc attgcctcca
     5041 cacgccatgg ggatttcgga gatgacaaga ctgtgagcct gtgcgtcaga cagctggaat
     5101 accagaacga agcaggagag accgtgcagg gagtctgctc cacatacctg tgcaatatca
     5161 aagagggcga tgacatcgcc attaccggcc cagtggggaa ggaaatgctg ctgcctccag
     5221 atgaggacgc aaacattgtc atgctggcca caggcactgg gatcgccccc tttcgggctt
     5281 tcctgtggag aatgttcaaa gaacagcacg aggactacaa gtttaaaggc ctggcctggc
     5341 tgatcttcgg gattccaaag tctgagaata tcctgtataa agatgacctg gaaaagatgg
     5401 cagccgagtt tcccgataac ttccgcctga catacgctat tagccgagaa cagcagaatg
     5461 cagagggggg acgaatgtat atccagcacc gggtggccga gaacgctgag gaactgtgga
     5521 acctgatgca gaatccaaaa acccatacat acatgtgcgg actgaagggc atggaacccg
     5581 ggatcgacga ggcctttacc gccctggctg aacagaatgg aaaggagtgg acaactttcc
     5641 agagagagat gaagaaagaa catagatggc acgtcgagac ctacatggct tcaatgacag
     5701 gaggacagca gatggggtag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     5761 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5821 gttatccgct aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     5881 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     5941 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     6001 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     6061 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
     6121 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
     6181 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
     6241 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
     6301 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
     6361 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6421 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6481 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6541 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc
     6601 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6661 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     6721 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg
     6781 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6841 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     6901 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6961 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7021 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7141 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7201 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7261 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7321 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7381 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7441 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7501 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7561 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7621 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7681 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7741 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7861 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7921 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7981 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8041 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8101 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8161 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8221 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8281 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8341 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8401 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8461 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8521 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8581 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8641 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8701 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8761 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8821 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8881 agtgccacct g
//
