LOCUS       sequence-194456-       11658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and 
            PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre 
            recombinase activity in Saccharomyces cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11658)
  AUTHORS   Hochrein L, Mitchell LA, Schulz K, Messerschmidt K, Mueller-Roeber B
  TITLE     L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination
            in yeast.
  JOURNAL   Nat Commun. 2018 May 22;9(1):1931. doi: 10.1038/s41467-017-02208-6.
  PUBMED    29789561
REFERENCE   2  (bases 1 to 11658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-22"; volume: "9(1):1931. doi"; pages: " 
            10.1038/s41467-017-02208-6"
COMMENT     Sequence Label: pLH_Scr15
FEATURES             Location/Qualifiers
     source          1..11658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(71..88)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(242..830)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(322..341)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1001..1792,1793..1861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1624..1643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    1959..2462
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(2601..2622)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2722..2990
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     misc_RNA        2999..3073
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      3078..3097
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     primer_bind     3187..3206
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(3398..4492)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             7179..7199
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(10863..11529)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(10970..10993)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 cggccctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
       61 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      121 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      181 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      241 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      301 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      361 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      421 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      481 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      541 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      601 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      661 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      721 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      781 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      841 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      901 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      961 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1021 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1081 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1141 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1201 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1261 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1321 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1381 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1441 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1501 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1561 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1621 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1681 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1741 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1801 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1861 tactcttcct ttttcaatgg gtaataactg atataattaa attgaagctc taatttgtga
     1921 gtttagtata catgcattta cttataatac agttgacgga tcgcttgcct gtaacttaca
     1981 cgcgcctcgt atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt
     2041 ttatgttttg tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga
     2101 agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata
     2161 tttattagac aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg
     2221 taaaatcaca ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat
     2281 taatacctga gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga
     2341 gtcttttaca tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct
     2401 atttttaatt tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg
     2461 atgaaaagga ccacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     2521 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     2581 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     2641 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     2701 cctaatcaag tttttggggt ctctttgaaa agataatgta tgattatgct ttcactcata
     2761 tttatacaga aacttgatgt tttctttcga gtatatacaa ggtgattaca tgtacgtttg
     2821 aagtacaact ctagattttg tagtgccctc ttgggctagc ggtaaaggtg cgcatttttt
     2881 cacaccctac aatgttctgt tcaaaagatt ttggtcaaac gctgtagaag tgaaagttgg
     2941 tgcgcatgtt tcggcgttcg aaacttctcc gcagtgaaag ataaatgatc gcggccgcgt
     3001 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
     3061 caccgagtcg gtggtgcttt ttttgttttt tatgtctagg tgccgtaaag cagtaaatcg
     3121 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3181 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3241 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtggggatg
     3301 atccactagt tattccattt tgtaatttcg tgtcgtttct attatgaatt tcatttataa
     3361 agtttatgta caaatatcat aaaaaaagag aatcttttta agcaaggatt ttcttaactt
     3421 cttcggcgac agcatcaccg acttcggtgg tactgttgga accacctaaa tcaccagttc
     3481 tgatacctgc atccaaaacc tttttaactg catcttcaat ggccttacct tcttcaggca
     3541 agttcaatga caatttcaac atcattgcag cagacaagat agtggcgata gggttgacct
     3601 tattctttgg caaatctgga gcagaaccgt ggcatggttc gtacaaacca aatgcggtgt
     3661 tcttgtctgg caaagaggcc aaggacgcag atggcaacaa acccaaggaa cctgggataa
     3721 cggaggcttc atcggagatg atatcaccaa acatgttgct ggtgattata ataccattta
     3781 ggtgggttgg gttcttaact aggatcatgg cggcagaatc aatcaattga tgttgaacct
     3841 tcaatgtagg aaattcgttc ttgatggttt cctccacagt ttttctccat aatcttgaag
     3901 aggccaaaac attagcttta tccaaggacc aaataggcaa tggtggctca tgttgtaggg
     3961 ccatgaaagc ggccattctt gtgattcttt gcacttctgg aacggtgtat tgttcactat
     4021 cccaagcgac accatcacca tcgtcttcct ttctcttacc aaagtaaata cctcccacta
     4081 attctctgac aacaacgaag tcagtacctt tagcaaattg tggcttgatt ggagataagt
     4141 ctaaaagaga gtcggatgca aagttacatg gtcttaagtt ggcgtacaat tgaagttctt
     4201 tacggatttt tagtaaacct tgttcaggtc taacactacc tgtaccccat ttaggaccac
     4261 ccacagcacc taacaaaacg gcatcagcct tcttggaggc ttccagcgcc tcatctggaa
     4321 gtggaacacc tgtagcatcg atagcagcac caccaattaa atgattttcg aaatcgaact
     4381 tgacattgga acgaacatca gaaatagctt taagaacctt aatggcttcg gctgtgattt
     4441 cttgaccaac gtggtcacct ggcaaaacga cgatcttctt aggggcagac attagaatgg
     4501 tatatccttg aaatatatat atatattgct gaaatgtaaa aggtaagaaa agttagaaag
     4561 taagacgatt gctaaccacc tattggaaaa aacaataggt ccttaaataa tattgtcaac
     4621 ttcaagtatt gtgatgcaag catttagtca tgaacgcttc tctattctat atgaaaagcc
     4681 ggttccggcg ctctcacctt tcctttttct cccaattttt cagttgaaaa aggtatatgc
     4741 gtcaggcgac ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat
     4801 agcgcccctg tgtgttctcg ttatgttgag gaaaaaaata atggttgcta agagattcga
     4861 actcttgcat cttacgatac ctgagtccat ttcattacct ctttctccgc acccgacata
     4921 gataaatagg aaagtctggt aactcaggac actgtatctg ctacgctgtt tatataacaa
     4981 tactgacagt actaaataat tgcctacttg gcttcacata cgttgcatac gtcgatatag
     5041 ataataatga taatgacagc aggattatcg taatacgtaa tagttgaaaa tctcaaaaat
     5101 gtgtgggtca ttacgtaaat aatgatagga atgggattct tctatttttc ctttttccat
     5161 tctagcagcc gtcgggaaaa cgtggcatcc tctctttcgg gctcaattgg agtcacgctg
     5221 ccgtgagcat cctctctttc catatctaac aactgagcac gtaaccaatg gaaaagcatg
     5281 agcttagcgt tgctccaaaa aagtattgga tggttaatac catttgtctg ttctcttctg
     5341 actttgactc ctcaaaaaaa aaaaatctac aatcaacaga tcgcttcaat tacgccctca
     5401 caaaaacttt tttccttctt cttcgcccac gttaaatttt atccctcatg ttgtctaacg
     5461 gatttctgca cttgatttat tataaaaaga caaagacata atacttctct atcaatttca
     5521 gttattgttc ttccttgcgt tattcttctg ttcttctttt tcttttgtca tatataacca
     5581 taaccaagta atacatattc aaaatgcctc tgtttgagct tttcaggctc accaaagcta
     5641 agcttgaatc tgctcaagac aggaaccctt ctccacctgt agatgaagtt gtggagctgg
     5701 tgtgggaaaa tggtcagata tcaactcaaa gtcagtcaag tagatcgagg aacattcctc
     5761 caccacaagc aaactcttct agagctagag agattggaaa tggctcaaag acgactatgg
     5821 tggacgagat ccctatgtca gtgccatcac taatgacggg tttgagtcaa gacgatgact
     5881 ttgttccatg gttgaatcat catccctccc ttgatggata ttgctctgat ttcttgcgtg
     5941 atgtgtcgtc tcctgttact gtcaacgagc aagagagtga tatggcggta aaccaaactg
     6001 ctttcccgtt gtttcagaga agaaaggatg gcaatgaatc agctcctgct gcttcttcgt
     6061 cgcagtataa cggtttccaa tcgcattctc tgtatggaag tgatagagct agagatcttc
     6121 ctagccaaca aaccaatccg gatcggttta ctcagacgca ggaaccacta attactagta
     6181 acaagcctag tttggtcaac ttttcacatt tcttacgccc tgcaactttt gcgaagacta
     6241 ctaataataa ccttcatgac actaaagaaa agagtcctca aagcccgcca aatgtgtttc
     6301 agaccagagt tcttggagct aaagactctg aagataaggt tcttaacgag tctgttgctt
     6361 ctgctacgcc taaagataac caaaaggctt gcctaatatc agaggactca tgtagaaaag
     6421 accaagagag tgaaaaagca gttgtatgtt cttctgttgg ctcgggtaat agtctcgatg
     6481 gcccatccga aagtccttca ctttctttaa agagaaagca ttcgaatatt caagacattg
     6541 actgtcatag tgaagatgtg gaagaagaat caggagatgg aagaaaggaa gcaggtccat
     6601 ctcgaacggg tttgggttca aagagaagcc gctctgcaga agtgcataat ctgtctgaaa
     6661 ggagacggcg tgataggatc aacgagaaga tgcgtgccct gcaagaactc attccaaact
     6721 gtaacaaggt ggacaaagct tcgatgctag atgaagccat cgagtatctc aagtcactcc
     6781 aacttcaagt gcagatcatg tcaatggcgt ctggttacta tctgccaccg gcggttatgt
     6841 tcccaccggg tatggggcat tacccggcag cagctgctgc aatggcaatg ggtatgggaa
     6901 tgccttatgc aatgggcttg cctgatttga gccgtggtgg ttcatcggtt aaccacggac
     6961 cacagttcca agtctcgggg atgcaacaac aaccagtggc gatgggtatt ccacgtgtct
     7021 ctggtggtgg tatctttgcc ggttcttcga cgattggcaa tggctcgact agagatttat
     7081 ctggttctaa agatcaaaca acgacgaata acaacagtaa cttgaaacca ataaagagaa
     7141 aacaggggtc ttctgatcag ttttgtggat cgtcgagccc caagaagaag agaaaggtgg
     7201 gtggcggtgg ctctggaggt ggtgggtccg gaggaggcgg ccgccgacca agtgacagca
     7261 atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg
     7321 caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa
     7381 atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct tataacaccc
     7441 tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg
     7501 ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga
     7561 aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag
     7621 ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat
     7681 ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgac
     7741 tgatttacgg cgctaaggat gactctggtc agaggtacct ggcctggtct ggacacagtg
     7801 cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc
     7861 aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg
     7921 aaacaggggc aatggtgcgc ctgctggaag atggcgatta ggttaattca aattaattga
     7981 tatagttttt taatgagtat tgaatctgtt tagaaataat ggaatattat ttttatttat
     8041 ttatttatat tattggtcgg ctcttttctt ctgaaggtca atgacaaaat gatatgaagg
     8101 aaataatgat ttctaaaatt ttacaacgta agatattttt acaaaagcct agctcatctt
     8161 ttgtcatgca ctattttact cacgcttgaa attaacggcc agtccactgc ggagtcattt
     8221 caaagtcatc ctaatcgatc tatcgttttt gatagctaaa gtgagattcg cacaataaac
     8281 gctgcttctg tttcatgtag atgatactga ctgcacgcaa atataggcat gatttatagg
     8341 catgatttga tggctgtacc gataggaacg ctaagagtaa cttcagaatc gttatcctgg
     8401 cggaaaaaat tcatttgtaa actttaaaaa aaaaagccaa tatccccaaa attattaaga
     8461 gcgcctccat tattaactaa aatttcactc agcatccaca atgtatcagg tatctactac
     8521 agatattaca tgtggcgaaa aagacaagaa caatgcaata gcgcatcaag aaaaaacaca
     8581 aagctttcaa tcaatgaatc gaaaatgtca ttaaaatagt atataaattg aaactaagtc
     8641 ataaagctat aaaaagaaaa tttatttaaa tgcaagattt aaagtaaatt cacctacagc
     8701 ccggaccgac gatgaagcat gtttagctgg cccaaatgtt gctggatagt ttttactgcc
     8761 agaccgcgcg cctgaagata tagaagataa tcgcgaacat cttcaggttc tgcgggaaac
     8821 catttccggt tattcaactt gcaccatgcc gcccacgacc ggcaaacgga cagaagcatt
     8881 ttccaggtat gctcagaaaa cgcctggcga tccctgaaca tgtccatcag gttcttgcga
     8941 acctcatcac tcgtgcggcc gcctcctccg gacccaccac ctccagagcc accgccacca
     9001 gattctttaa aagagtctct cagaataagc tggagcgagt gaatcgcatc catttccgca
     9061 gtttcccatg gttgactccg gctcttaaca acttcaagaa aagcctgaaa ggacgaacga
     9121 ggatgcattc gttgcccatc atctttatcc tccggatgat gcttagcgcc tccccatttg
     9181 atttctttcg cagtgtgaga tcgaaaccaa aaaagaaagt ctctttttgt gatatatgca
     9241 actgccatac cgcacacagc atcccctaac gcagctgcac cgggataccc cgcatcgcct
     9301 aaactatcag tgcttaatcc ggttgaatcc gcatgattcg caagcaacca ctccacaaca
     9361 tcttttatct gaacttcact aggagcaaca cccaacgggt aatacttccc gtggtaaaga
     9421 aatgctgcac cgtcacattt cactaagtcc atgatactgg gactctgtgt aacaattcca
     9481 gcaggcgagt cacgcagaag catatcacat aacagtgtct gcgttctcaa aacgcgtttc
     9541 tctgacattt gcaaagctaa ctgcaattcc atgtttaact gtaaaccgaa agcctgcatc
     9601 aaaaactcac aagcatacct tagcggaaac ggtatgcagc gagaagaagt gtgatggcaa
     9661 acaaccaaac cccaaagcct catcgagctt cttccactag ctacattgct cccatcatct
     9721 tcatttccat tgattataac cgccattgct aaagacgcaa tagatcccat gttagccata
     9781 tactgagagt gacaaccatg aggagcccta agagtagaac caaccaagca catagactga
     9841 gttagcctat cgtcctggac cacaagaaca ggtgtggcat tgcaatctac tatcattcgg
     9901 acacggttct gcttaaacaa gaaccttgac gcttgaggaa tatcagtagc aggataatgc
     9961 agtccaatat aaggctctaa atcatctcgt ttactctcag ctacaacttc tccatgctca
    10021 tcttcatgaa acttataaac cataacacga tcataaccag tcaagtccct cacactttcc
    10081 acgacagtgt cacacaaaag cttaatatct ccaccaggaa gagcctgtaa ctgagaaatc
    10141 gcacgaaccg cgagtttctg cgattgaaca gcaccagcaa tagaaagcgc aggatcttca
    10201 gttctagctg gctctaaatc aataacaaca ccaacatcaa tcctatgaag aatggcgtaa
    10261 aacggtttac cagtattctt ggaatgtatc caaaccggat ttaacaaggt aatctctcga
    10321 gcaacgaaag cacgctcgag tagaatcgag ctcgaagaag tgaacaaaga tctcacatca
    10381 gttcccatag ctagaatctc aggtttctca agagtaggaa cagattgagg cataatccct
    10441 aacatttctc tggcgttttc actgtaaccg atgatccgga aactggattc atcgacggcg
    10501 atcatacatc cgaaaggctg aatgtaacca cctcgctgga ttcgagagag ataagctgtg
    10561 atctgttgct caggtacaga ggaaccgtac gtcgtcgttt tgagtgattg tgagtagtcg
    10621 aatgatttcc ctgattcgcc ggattgttcg aaaacggcgt ggagtcttgc gtcgacggtg
    10681 tactgttgaa ttgctttgct cattgattca gtgttgcttc ttggtctgag agatttcgtt
    10741 cccgacgatt gagcttgttc tcctcctctt cggttattag gagtgtgact tgacgacggt
    10801 tcttcttctc cgccacggcc accgccacgg ccaccgccac tacccccgac tccggaaacc
    10861 attttgtttg tttatgtgtg tttattcgaa actaagttct tggtgtttta aaactaaaaa
    10921 aaagactaac tataaaagta gaatttaaga agtttaagaa atagatttac agaattacaa
    10981 tcaataccta ccgtctttat atacttatta gtcaagtagg ggaataattt cagggaactg
    11041 gtttcaacct tttttttcag ctttttccaa atcagagaga gcagaaggta atagaaggtg
    11101 taagaaaatg agatagatac atgcgtgggt caattgcctt gtgtcatcat ttactccagg
    11161 caggttgcat cactccattg aggttgtgcc cgttttttgc ctgtttgtgc ccctgttctc
    11221 tgtagttgcg ctaagagaat ggacctatga actgatggtt ggtgaagaaa acaatatttt
    11281 ggtgctggga ttcttttttt ttctggatgc cagcttaaaa agcgggctcc attatattta
    11341 gtggatgcca ggaataaact gttcacccag acacctacga tgttatatat tctgtgtaac
    11401 ccgcccccta ttttgggcat gtacgggtta cagcagaatt aaaaggctaa ttttttgact
    11461 aaataaagtt aggaaaatca ctactattaa ttatttacgt attctttgaa atggcagtat
    11521 tgataatgat aaacttagat tctgtgaaac tctcaataaa gggacttcgt ctaaggtttc
    11581 agtcacacta cggatacttt tacaacggga gcagttattc aatctatgtc gggtgcggag
    11641 aaagaggtaa tgaaatgg
//
