LOCUS       sequence-194458-        8846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expreses AURKB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 8846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7052 pHAGE-P-CMVt-N-HA-GAW-AURKB
FEATURES             Location/Qualifiers
     source          1..8846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     514..533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     767..784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1024..1045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1060..1065,1066..1083,1084..1090)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1098..1114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1103..1125
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1122..1138
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1122..1138
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1188..1207
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1399..2032
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2079..2204
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2701..2934
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3119..3163
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3456..3573
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3625..3928
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3929..4132
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4083..4103
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4134..4152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4155..4173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4183..4207
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4294..4303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4306..4332
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4348..4372
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5104..5115
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5405..5429)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5448..5947
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5483..5506)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5880..5899
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5968..6567
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5968..5987)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6464..6484
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6634..7222
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6687..6707)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7105..7116)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7297..7530
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7649..7667)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7735..7839
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7840..7908,7909..8700)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8058..8077)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
       61 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      781 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      841 ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      901 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      961 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     1021 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     1081 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
     1141 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1201 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     1261 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     1321 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     1381 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     1441 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     1501 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     1561 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     1621 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     1681 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     1741 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     1801 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     1861 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     1921 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     1981 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     2041 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     2101 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     2161 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     2221 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     2281 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     2341 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     2401 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     2461 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     2521 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     2581 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     2641 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     2701 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     2761 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     2821 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     2881 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     2941 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     3001 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     3061 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     3121 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     3181 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     3241 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     3301 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     3361 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     3421 tcgccgaatt cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     3481 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     3541 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     3601 ccagtttgga ctagtggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     3661 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3721 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3781 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3841 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3901 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3961 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     4021 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     4081 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat
     4141 cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg
     4201 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
     4261 atccagcctc cggactctag cgtttaaacg ctagccacca tggcgtaccc atacgatgtt
     4321 ccagattacg ctgctcgagg ttatcaaaca agtttgtaca aaaaagttgg catggcccag
     4381 aaggagaact cctacccctg gccctacggc cgacagacgg ctccatctgg cctgagcacc
     4441 ctgccccagc gagtcctccg gaaagagcct gtcaccccat ctgcacttgt cctcatgagc
     4501 cgctccaatg tccagcccac agctgcccct ggccagaagg tgatggagaa tagcagtggg
     4561 acacccgaca tcttaacgcg gcacttcaca attgatgact ttgagattgg gcgtcctctg
     4621 ggcaaaggca agtttggaaa cgtgtacttg gctcgggaga agaaaagcca tttcatcgtg
     4681 gcgctcaagg tcctcttcaa gtcccagata gagaaggagg gcgtggagca tcagctgcgc
     4741 agagagatcg aaatccaggc ccacctgcac catcccaaca tcctgcgtct ctacaactat
     4801 ttttatgacc ggaggaggat ctacttgatt ctagagtatg ccccccgcgg ggagctctac
     4861 aaggagctgc agaagagctg cacatttgac gagcagcgaa cagccacgat catggaggag
     4921 ttggcagatg ctctaatgta ctgccatggg aagaaggtga ttcacagaga cataaagcca
     4981 gaaaatctgc tcttagggct caagggagag ctgaagattg ctgacttcgg ctggtctgtg
     5041 catgcgccct ccctgaggag gaagacaatg tgtggcaccc tggactacct gcccccagag
     5101 atgattgagg ggcgcatgca caatgagaag gtggatctgt ggtgcattgg agtgctttgc
     5161 tatgagctgc tggtggggaa cccacccttt gagagtgcat cacacaacga gacctatcgc
     5221 cgcatcgtca aggtggacct aaagttcccc gcttccgtgc ccatgggagc ccaggacctc
     5281 atctccaaac tgctcaggca taacccctcg gaacggctgc ccctggccca ggtctcagcc
     5341 cacccttggg tccgggccaa ctctcggagg gtgctgcctc cctctgccct tcaatctgtc
     5401 gcctgcccaa ctttcttgta caaagtggtt cgataacgaa ttctaccggg taggggaggc
     5461 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     5521 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     5581 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     5641 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     5701 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     5761 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     5821 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     5881 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     5941 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     6001 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6061 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6121 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6181 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6241 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6301 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6361 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6421 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6481 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6541 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     6601 aggagcgcac gaccccatgc atcgatagat cctaatcaac ctctggatta caaaatttgt
     6661 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6721 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6781 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6841 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6901 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6961 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7021 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7081 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7141 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7201 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
     7261 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7321 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7381 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7441 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7501 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7561 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
     7621 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     7681 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     7741 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7801 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     7861 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     7921 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     7981 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8041 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     8101 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8161 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8221 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8281 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8341 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     8401 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8461 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8521 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8581 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8641 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8701 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8761 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8821 ttttcgttcc actgagcgtc agaccc
//
