LOCUS       sequence-194459-       10379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BICD2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7053 pHAGE-P-CMVt-N-HA-GAW-BICD2
FEATURES             Location/Qualifiers
     source          1..10379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      30..618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     519..538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     772..789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1029..1050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1065..1070,1071..1088,1089..1095)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1103..1119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1108..1130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1127..1143
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1127..1143
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1193..1212
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1404..2037
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2084..2209
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2706..2939
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3124..3168
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3461..3578
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3630..3933
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3934..4137
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4088..4108
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4139..4157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4160..4178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4188..4212
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4299..4308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4311..4337
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4353..4377
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6943..6967)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6986..7485
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7021..7044)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7418..7437
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7506..8105
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7506..7525)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8002..8022
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8172..8760
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8225..8245)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8643..8654)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8835..9068
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9187..9205)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9273..9377
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9378..9446,9447..10238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9596..9615)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
       61 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      601 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      661 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
      721 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      781 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      841 attcattaat gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg
      901 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
      961 tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     1021 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     1081 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     1141 gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1201 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     1261 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     1321 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     1381 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     1441 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     1501 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     1561 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     1621 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     1681 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     1741 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     1801 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     1861 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     1921 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     1981 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     2041 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     2101 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     2161 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     2221 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     2281 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     2341 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     2401 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     2461 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     2521 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     2581 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     2641 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     2701 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     2761 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     2821 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     2881 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     2941 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3001 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     3061 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     3121 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     3181 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     3241 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     3301 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     3361 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     3421 cggtatcgcc gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     3481 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     3541 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     3601 gagatccagt ttggactagt ggagttccgc gttacataac ttacggtaaa tggcccgcct
     3661 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     3721 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     3781 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     3841 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     3901 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     3961 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     4021 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     4081 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     4141 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
     4201 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     4261 gaccgatcca gcctccggac tctagcgttt aaacgctagc caccatggcg tacccatacg
     4321 atgttccaga ttacgctgct cgaggttatc aaacaagttt gtacaaaaaa gttggcatgt
     4381 cggcgccgtc ggaggaggag gagtacgcgc ggctggtgat ggaggcgcag ccggagtggc
     4441 tgcgcgccga ggtgaagcgg ctgtcccacg agctggccga gaccacgcgt gagaagatcc
     4501 aggcggccga gtacgggctg gcggtgctcg aggagaagca ccagctcaag ctgcagttcg
     4561 aggagctcga ggtggactat gaggctatcc gcagcgagat ggagcagctc aaggaggcct
     4621 ttggacaagc acacacaaac cacaagaagg tggctgctga cggagagagc cgggaggaga
     4681 gcctgatcca ggagtcggcc tccaaggagc agtactacgt gcggaaggtg ctagagctgc
     4741 agacggagct gaagcagttg cgcaatgtcc tcaccaacac gcagtcggag aatgagcgcc
     4801 tggcctctgt ggcccaggag ctgaaggaga tcaaccagaa tgtggagatc cagcgtggcc
     4861 gcctgcggga tgacatcaag gagtacaaat tccgggaagc tcgtctgctg caggactact
     4921 cggaactgga ggaggagaac atcagcctgc agaagcaagt gtctgtgctc agacagaacc
     4981 aggtggagtt tgagggcctc aagcatgaga tcaagcgtct ggaggaggag accgagtacc
     5041 tcaacagcca gctggaggat gccatccgcc tcaaggagat ctcagagcgg cagctggagg
     5101 aggcgctgga gaccctgaag acggagcgcg aacagaagaa cagcctgcgc aaggagctgt
     5161 cacactacat gagcatcaat gactccttct acaccagcca cctgcatgtc tcgctggatg
     5221 gcctcaagtt cagtgacgat gctgccgagc ccaacaacga tgccgaggcc ctggtcaatg
     5281 gctttgagca cggcggcctg gccaagctgc cactggacaa caagacctcc acgcccaaga
     5341 aggagggcct cgcaccgccc tcccccagcc tcgtctccga cctactcagt gagctcaaca
     5401 tctctgagat ccagaagctg aagcagcagc tgatgcagat ggagcgggaa aaggcgggcc
     5461 tgctggcaac gctgcaggac acacagaagc agctggagca cacgcggggc tccctgtcag
     5521 aacagcagga gaaggtgacc cgcctcacag agaatctgag tgccctgcgg cgcctgcagg
     5581 ccagcaagga gcggcagaca gccctggaca acgagaagga ccgtgacagc catgaggatg
     5641 gggactacta cgaggtggac atcaacgggc ctgagatctt ggcctgcaag taccatgtgg
     5701 ctgtggctga ggctggcgag ctccgcgagc agctcaaggc actgcgcagc acgcacgagg
     5761 ctcgtgaggc ccagcacgcc gaggagaagg gccgctatga ggctgagggc caggcactca
     5821 cggagaaggt ctccctgcta gagaaggcca gccgccagga ccgcgagctg ctggcccggc
     5881 tggagaagga gctaaagaag gtgagcgacg tcgccggcga gacacagggc agcctgagtg
     5941 tggcccagga tgagctggtg accttcagtg aggagctggc caatctctac caccacgtgt
     6001 gcatgtgcaa caatgagaca cccaaccgtg tcatgctgga ctactaccgc gagggccagg
     6061 gcggggccgg ccgcaccagt cccgggggcc gcaccagccc cgaggcgcgt ggccggcgct
     6121 cacccatcct cctacccaag gggctgctgg ctcctgaggc gggccgagca gatggtggga
     6181 cgggggacag cagcccctcg cctggctcct cactgccatc acccctgagt gacccacgcc
     6241 gggagcccat gaacatctac aacctgatcg ctatcatccg tgaccagatc aagcacctgc
     6301 aggcagccgt ggaccgcacc acggagctgt cacgccagcg cattgcctct caggagctgg
     6361 gccccgccgt ggacaaggac aaggaagcgc ttatggagga gatcctcaag ctgaagtcgc
     6421 tgctcagcac caagcgggag cagatcacca cgctgcgcac tgtgctcaag gccaacaagc
     6481 agacggccga ggtggccctt gccaacctga agagcaagta tgagaatgag aaggccatgg
     6541 ttaccgagac catgatgaag ctgcgcaatg agctcaaggc cctcaaggag gacgcagcca
     6601 ccttctcctc gctgcgtgct atgtttgcca ccaggtgtga cgagtacatt acacagctgg
     6661 atgagatgca gcggcagctg gcggctgctg aggacgagaa gaagacgctg aactcgctgc
     6721 tgcgcatggc catccagcag aagctggcgc tgacccagcg gctggagctg ctcgagctgg
     6781 accatgagca gacccggcgt ggccgtgcca aagccgcccc gaagaccaag ccagccacac
     6841 cgagcgtaag tcacacctgt gcctgtgcca gcgacagggc cgagggcacc gggctggcca
     6901 accaggtgtt ctgcagcgag aagcacagca tttactgtga ttacccaact ttcttgtaca
     6961 aagtggttcg ataacgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     7021 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     7081 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     7141 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     7201 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     7261 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     7321 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     7381 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     7441 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
     7501 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     7561 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     7621 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     7681 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     7741 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     7801 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     7861 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     7921 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     7981 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     8041 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     8101 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     8161 cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     8221 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     8281 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     8341 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     8401 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     8461 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     8521 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     8581 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     8641 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8701 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8761 ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     8821 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     8881 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8941 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9001 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9061 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     9121 aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac
     9181 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     9241 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9301 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9361 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9421 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9481 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9541 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9601 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     9661 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9721 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9781 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9841 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9901 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9961 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10021 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10081 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10141 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10201 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10261 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10321 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtca
//
