LOCUS       sequence-194460-        8738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses COPE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7054 pHAGE-P-CMVt-N-HA-GAW-COPE
FEATURES             Location/Qualifiers
     source          1..8738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     503..522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     756..773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1013..1034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1049..1054,1055..1072,1073..1079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1087..1103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1092..1114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1111..1127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1111..1127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1177..1196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1388..2021
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2068..2193
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2690..2923
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3108..3152
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3445..3562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3614..3917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3918..4121
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4072..4092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4123..4141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4144..4162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4172..4196
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4283..4292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4295..4321
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4337..4361
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5286..5310)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5329..5828
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5364..5387)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5761..5780
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5849..6448
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5849..5868)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6345..6365
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6515..7103
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6568..6588)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6986..6997)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7178..7411
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7530..7548)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7616..7720
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7721..7789,7790..8581)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7939..7958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      781 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca
      841 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
      901 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
      961 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     1021 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     1081 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
     1141 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1201 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     1261 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     1321 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     1381 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     1441 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     1501 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     1561 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     1621 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     1681 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     1741 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     1801 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     1861 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     1921 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     1981 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     2041 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     2101 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     2161 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     2221 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     2281 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     2341 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     2401 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     2461 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     2521 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     2581 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     2641 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     2701 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     2761 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     2821 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     2881 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     2941 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3001 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3061 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3121 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3181 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3241 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3301 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3361 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc
     3421 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     3481 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     3541 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac
     3601 tagtggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3661 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3721 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3781 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3841 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3901 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3961 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     4021 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     4081 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag
     4141 atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct
     4201 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
     4261 ggactctagc gtttaaacgc tagccaccat ggcgtaccca tacgatgttc cagattacgc
     4321 tgctcgaggt tatcaaacaa gtttgtacaa aaaagttggc atggcgcctc cggcccccgg
     4381 cccggcctcc ggcggctccg gggaggtaga cgagctgttc gacgtaaaga acgccttcta
     4441 catcggcagc taccagcagt gcataaacga ggcgcagcgg gtgaagctgt caagcccaga
     4501 gagagacgtg gagagggacg tcttcctgta tagagcgtac ctggcgcaga ggaagttcgg
     4561 tgtggtcctg gatgagatca agccctcctc ggcccctgag ctccaggccg tgcgcatgtt
     4621 tgctgactac ctcgcccacg agagtcggag ggacagcatc gtggccgagc tggaccgaga
     4681 gatgagcagg agcgtggacg tgaccaacac caccttcctg ctcatggccg cctccatcta
     4741 tctccacgac cagaacccgg atgccgccct gcgtgcgctg caccaggggg acagcctgga
     4801 gtgcacagcc atgacagtgc agatcctgct gaagctggac cgcctggacc tcgcccggaa
     4861 ggagctgaag agaatgcagg acctggacga ggatgccacc ctcacccagc tcgccactgc
     4921 ctgggtcagc ctggccacgg gtggtgagaa gctgcaggat gcctactaca tcttccagga
     4981 gatggctgac aagtgctcgc ccaccctgct gctgctcaat gggcaggcgg cctgccacat
     5041 ggcccagggc cgctgggagg ccgctgaggg cctgctgcag gaggcgctag acaaggatag
     5101 tggctacccg gagacgctgg tcaacctcat cgtcctgtcc cagcacctgg gcaagccccc
     5161 tgaggtgaca aaccgatacc tgtcccagct gaaggatgcc cacaggtccc atcccttcat
     5221 caaggagtac caggccaagg agaacgactt tgacaggctg gtgctacagt acgctcccag
     5281 cgcctgccca actttcttgt acaaagtggt tcgataacga attctaccgg gtaggggagg
     5341 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     5401 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     5461 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     5521 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     5581 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     5641 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     5701 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     5761 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     5821 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     5881 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     5941 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6001 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6061 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6121 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6181 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6241 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6301 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6361 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6421 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     6481 aaggagcgca cgaccccatg catcgataga tcctaatcaa cctctggatt acaaaatttg
     6541 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6601 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6661 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6721 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6781 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6841 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6901 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     6961 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7021 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7081 ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct
     7141 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7201 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     7261 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7321 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7381 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7441 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa
     7501 agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7561 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7621 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7681 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7741 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7801 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7861 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7921 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     7981 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8041 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8101 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8161 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8221 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8281 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8341 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8401 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8461 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8521 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8581 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8641 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8701 gttttcgttc cactgagcgt cagaccccgt agaaaaga
//
