LOCUS       sequence-194464-        9148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EIF3E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 9148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7135 pHAGE-P-CMVt-N-HA-GAW-EIF3E
FEATURES             Location/Qualifiers
     source          1..9148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      22..610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     511..530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     764..781
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1021..1042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1057..1062,1063..1080,1081..1087)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1095..1111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1100..1122
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1119..1135
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1119..1135
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1185..1204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1396..2029
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2076..2201
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2698..2931
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3116..3160
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3453..3570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3622..3925
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3926..4129
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4080..4100
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4131..4149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4152..4170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4180..4204
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4291..4300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4303..4329
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4345..4369
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5705..5729)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5748..6246
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5783..5806)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6179..6198
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6267..6866
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6267..6286)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6763..6783
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6933..7521
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6986..7006)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7404..7415)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7596..7829
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7948..7966)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8034..8138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8139..8207,8208..8999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8357..8376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
       61 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
      181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      661 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      721 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      781 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      841 atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
      901 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
      961 aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1021 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1081 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
     1141 tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1201 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     1261 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     1321 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     1381 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
     1441 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     1501 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     1561 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     1621 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     1681 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     1741 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     1801 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     1861 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     1921 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     1981 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     2041 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     2101 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2161 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     2221 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     2281 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     2341 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     2401 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     2461 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     2521 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     2581 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     2641 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     2701 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     2761 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     2821 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     2881 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     2941 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     3001 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     3061 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     3121 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     3181 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     3241 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     3301 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     3361 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     3421 ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     3481 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     3541 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     3601 gtttggacta gtggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     3661 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     3721 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     3781 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     3841 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     3901 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3961 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     4021 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     4081 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
     4141 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
     4201 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     4261 cagcctccgg actctagcgt ttaaacgcta gccaccatgg cgtacccata cgatgttcca
     4321 gattacgctg ctcgaggtta tcaaacaagt ttgtacaaaa aagttggcat ggcggagtac
     4381 gacttgacta ctcgcatcgc gcactttttg gatcggcatc tagtctttcc gcttcttgaa
     4441 tttctctctg taaaggagat atataatgaa aaggaattat tacaaggtaa attggacctt
     4501 cttagtgata ccaacatggt agactttgct atggatgtat acaaaaacct ttattctgat
     4561 gatattcctc atgctttgag agagaaaaga accacagtgg ttgcacaact gaaacagctt
     4621 caggcagaaa cagaaccaat tgtgaagatg tttgaagatc cagaaactac aaggcaaatg
     4681 cagtcaacca gggatggtag gatgctcttt gactacctgg cggacaagca tggttttagg
     4741 caggaatatt tagatacact ctacagatat gcaaaattcc agtacgaatg tgggaactac
     4801 tcaggagcag cagaatatct ttattttttt agagtgctgg ttccagcaac agatagaaat
     4861 gctttaagtt cactctgggg aaagctggcc tctgaaatct taatgcagaa ttgggatgca
     4921 gccatggaag accttacacg gttaaaagag accatagata ataattctgt gagttctcca
     4981 cttcagtctc ttcagcagag aacatggctc attcactggt ctctgtttgt tttcttcaat
     5041 caccccaaag gtcgcgataa tattattgac ctcttccttt atcagccaca atatcttaat
     5101 gcaattcaga caatgtgtcc acacattctt cgctatttga ctacagcagt cataacaaac
     5161 aaggatgttc gaaaacgtcg gcaggttcta aaagatctag ttaaagttat tcaacaggag
     5221 tcttacacat ataaagaccc aattacagaa tttgttgaat gtttatatgt taactttgac
     5281 tttgatgggg ctcagaaaaa gctgagggaa tgtgaatcag tgcttgtgaa tgacttcttc
     5341 ttggtggctt gtcttgagga tttcattgaa aatgcccgtc tcttcatatt tgagactttc
     5401 tgtcgcatcc accagtgtat cagcattaac atgttggcag ataaattgaa catgactcca
     5461 gaagaagctg aaaggtggat tgtaaatttg attagaaatg caagactgga tgccaagatt
     5521 gattctaaat taggtcatgt ggttatgggt aacaatgcag tctcacccta tcaacaagtg
     5581 attgaaaaga ccaaaagcct ttcctttaga agccagatgt tggccatgaa tattgagaag
     5641 aaacttaatc agaatagcag gtcagaggct cctaactggg caactcaaga ttctggcttc
     5701 tactgcccaa ctttcttgta caaagtggtt cgataacgaa ttctaccggg taggggaggc
     5761 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     5821 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     5881 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     5941 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     6001 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     6061 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     6121 cggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     6181 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     6241 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     6301 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     6361 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     6421 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     6481 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     6541 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     6601 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     6661 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     6721 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     6781 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     6841 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     6901 ggagcgcacg accccatgca tcgatagatc ctaatcaacc tctggattac aaaatttgtg
     6961 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7021 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7081 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7141 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7201 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7261 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7321 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7381 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7441 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7501 ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt
     7561 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7621 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7681 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7741 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7801 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7861 tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag
     7921 ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7981 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8041 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8101 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8161 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8221 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8281 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8341 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     8401 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8461 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8521 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8581 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8641 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8701 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8761 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8821 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8881 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8941 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9001 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9061 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9121 tttcgttcca ctgagcgtca gaccccgt
//
