LOCUS       sequence-194468-        9022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector to introduce an osTir1 expressing cassette at the 
            mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). 
            Auxin-inducible degron system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur 
            N, Dekker J, Mirny LA, Bruneau BG
  TITLE     Targeted Degradation of CTCF Decouples Local Insulation of 
            Chromosome Domains from Genomic Compartmentalization.
  JOURNAL   Cell. 2017 May 18;169(5):930-944.e22. doi: 
            10.1016/j.cell.2017.05.004.
  PUBMED    28525758
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.05"; journalName: "Cell"; date: "2017-05-18- 
            18"; volume: "169"; issue: "5"; pages: "930-944.e22"
COMMENT     Sequence Label: pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor
FEATURES             Location/Qualifiers
     source          1..9022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1753..3477
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             3478..3519
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3529..3582
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3583..4182
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3583..3602)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(4210..4227)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4216..4423
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5386..5403)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5386..5402)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5395..5417)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5611..5630)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5730..5752
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5790..5808)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5876..5980
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5981..6049,6050..6841)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6199..6218)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7012..7600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7501..7520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7754..7771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7888..7909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7924..7929,7930..7947,7948..7954)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7962..7978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7967..7989
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7986..8002
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7986..8002
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgagaattct
     1741 cacgcgtcca ccatgacata cttccctgag gaggtggtcg aacatatttt ctcattcctt
     1801 cccgcccaac gagataggaa taccgtgtcc ctggtgtgca aggtctggta tgagatcgaa
     1861 cgcttgagcc gcagaggtgt ttttgtgggt aattgttatg ccgtacgagc cgggcgagtg
     1921 gccgcccggt tcccgaacgt cagggccctt acagtgaagg gcaagcctca ctttgccgac
     1981 ttcaatttgg tgcctccgga ctggggcggc tacgccgggc cctggatcga ggccgctgcg
     2041 cgcgggtgtc atggtctgga agaactcagg atgaaaagga tggtggtgtc agacgagtca
     2101 ctggaactgc ttgcccgcag cttcccccga ttccgcgccc tggtgctgat cagctgtgaa
     2161 ggatttagca ccgacggtct ggctgctgta gctagccatt gtaaactgct gcgagaactg
     2221 gacctgcagg aaaatgaggt agaggatagg ggacctcggt ggctgagctg tttcccggat
     2281 agctgcacta gcctggtgag cttgaatttc gcttgtatca agggcgaagt gaatgccggg
     2341 agtttggaga gactcgtttc ccgatccccc aacctgagat ctctgcggct gaacagatcc
     2401 gtttctgtgg acacgctcgc caagattttg ctccggactc ccaacctgga ggatctgggg
     2461 accggaaatc tgaccgatga tttccagaca gagagctact ttaagctcac tagtgctctg
     2521 gaaaaatgca aaatgcttag gtcactgagc gggttttggg acgctagccc tgtctgcctg
     2581 tcttttatct atcccctgtg tgcacagttg accggactta atctgagtta cgctccaacc
     2641 ctcgacgcta gtgaccttac taagatgatc agccgctgcg tgaagctcca aaggctctgg
     2701 gtactggatt gtatttcaga caagggactg caagttgtgg cctcctcctg caaagatctg
     2761 caggagctga gggtgttccc ttcagatttc tatgtggccg gatacagcgc agtcaccgag
     2821 gagggattgg tagcagtgtc cctggggtgc cccaaactca atagtttgct gtacttctgt
     2881 catcagatga ccaatgctgc ccttgttacc gttgccaaaa actgtccaaa cttcacaagg
     2941 tttagactgt gcatcttgga gcccggtaag cccgacgtcg taacgtcaca gccactggac
     3001 gaagggttcg gtgccatagt acgcgaatgt aagggtctcc agcggctgag catatccggc
     3061 ctgctgactg acaaagtgtt catgtatatt gggaagtacg ccaagcagct ggaaatgctg
     3121 tcaatcgcat tcgccggaga ttcagataaa gggatgatgc acgtgatgaa cgggtgcaag
     3181 aacctgagaa aactcgaaat tcgcgattcc ccttttgggg acgccgcact gctgggcaat
     3241 ttcgcccggt acgagacgat gaggtcactt tggatgtcaa gctgcaacgt gaccctcaag
     3301 ggatgccaag tcctggctag taaaatgcca atgctgaatg ttgaggtcat caacgaacgc
     3361 gacggtagta atgaaatgga ggaaaaccac ggcgatctgc ccaaagtgga gaaactgtac
     3421 gtctatcgga ccacagctgg cgcacgagat gacgctccca atttcgtgaa aattctgggc
     3481 aagcccatcc ccaaccccct gctgggcctg gacagcaccg gcagtggaga gggcagagga
     3541 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caatgaccga gtacaagccc
     3601 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     3661 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     3721 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     3781 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     3841 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     3901 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     3961 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     4021 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccac
     4081 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     4141 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagaattcta actagagctc
     4201 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4261 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4321 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4381 gcaaggggga ggattgggaa gagaatagca ggcatgctgg ggagcggccg ccctgcagga
     4441 tttaaatgtt aggtgttatg gcagtaactt taattctagc gatctggagg cagaggcagg
     4501 tggatctatg agtttgaggc tggcttgctc tacacagtga gttccagaaa agccagggct
     4561 acacagtgag accctatcta gaaacaacaa cagcaacaaa aaatatacac atctctccta
     4621 aaaataaaac acgcagtctg gaagtaagtg accgttgggg aagcactgga aaggagttag
     4681 agaatgtaga cctcttgtgc ctgactgggt gtccggattc aatagtgact tctccggtga
     4741 tctgggaatc tgtgaagagg ttctggctct ctgtctagcc tgaggctgat accccaactc
     4801 attttcttgt cttctgtgag gaaggttaca actctaggag tccccttcct ttggccagtg
     4861 cttccctaag aaagctatgg cttgattccc acccccaccc tacctccact gccaagtctc
     4921 tggacagatg tgcagcctct ggcagtggga gggaaggaac tggataaagt ggtcctcatt
     4981 cgtcagcctc actggcaggc ccggaaagtg ctgaactcag ggggctccgg agaggatgat
     5041 gcagaggtgg gcctcttccg aggccctgag tctagggttg ccactgagtc ttcctgagtc
     5101 attttctttg cccatggaag aaaagaaaag aaatttaaaa aacaaaaaca aaacaaaaaa
     5161 ccctaagtgg tttttggtgt aaggatctgg gagttcctcc tgttcgaagc tgttgggtac
     5221 cttcctacct cttcctacca cctcgccaga tttcagattc cgaccttcag tggctcaaat
     5281 cccacttccc taacttccta gcagtctgac ctcactttga gtgataacac atatgagcct
     5341 cagtttcaaa tgctagtatc catctcctag gggcagtatt taaatactgg ccgtcgtttt
     5401 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     5461 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     5521 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     5581 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5641 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5701 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5761 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5821 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5881 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5941 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6001 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6061 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6121 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6181 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6241 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6301 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6361 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6421 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6481 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6541 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6601 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6661 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6721 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6781 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6841 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6901 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6961 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7021 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7081 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7141 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7201 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7261 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7321 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7381 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7441 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7501 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7561 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7621 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7681 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7741 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7801 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     7861 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     7921 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     7981 tcacacagga aacagctatg accatgatta cgccatttaa atgaaaagtg gagaggcgtg
     8041 agtcggagaa ctaagaagaa acgcctcctc cagggctgag ctccgcccct aggctggcgt
     8101 gctgcgtact cccccagtac tcttgggggt gaggcgtttg tcagaaggag gcgctcagct
     8161 actttataca cccggaggag ggctggctcc gcccccagga gggacggtta ctagcagaat
     8221 gcagacggtg ctctgcatct ttttgtcaag ggacggtgtt ctgatccacg aaaccctccc
     8281 tcggagagtt cgggcccctc cgggccagtg tcctgcaaac aacctctcct gagcttgagg
     8341 aaagacaaga accgtttcca aacccaggtg attcggccac gcccccaagt taaggttccg
     8401 ccctgtacgt tcccaggtaa gctccgcctt gatcccgtcc cactggtaag aaaaggccct
     8461 gtgataggtg tgctctggtt tgcaggctcg cacccagctg gggccccttc ccatgctgct
     8521 ctgggataaa ggcttttcaa gccgcgctcc agagcttggc cttcacaaag aacttcaagg
     8581 cagtaacttt aattctttag cttctccact aggacctctc cttttcacta gttaactctc
     8641 aggtccccca gtgaactcac agtggccagt cacttgttca cttttgctgg gcctcttaga
     8701 actctaattt acgtaccccc tcctgggcct ccgcaagact cacaaaagac tcaacccttt
     8761 cccaagtcat ccctcccatc taatggtgca agcacacaat ctcaggcagc caggacccac
     8821 aaagtgaaga gtaaaaacgc actctgctgg gcagtggtgg cacacacctt taatcccgat
     8881 gctcaggagg cagaggctgg cagtccttga gttccaggcc agcctggtct acagagtgag
     8941 ttctaggaca gccaggacta cactgaaaaa ccctatctca aaacaaacag aaaacatccc
     9001 aaaatttaaa tggcgcgccg tc
//
