LOCUS       sequence-194470-        8882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EIF3EH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7136 pHAGE-P-CMVt-N-HA-GAW-EIF3H
FEATURES             Location/Qualifiers
     source          1..8882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      21..609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     510..529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     763..780
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1020..1041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1056..1061,1062..1079,1080..1086)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1094..1110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1099..1121
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1118..1134
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1118..1134
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1184..1203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1395..2028
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2075..2200
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2697..2930
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3115..3159
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3452..3569
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3621..3924
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3925..4128
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4079..4099
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4130..4148
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4151..4169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4179..4203
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4290..4299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4302..4328
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4344..4368
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5425..5449)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5503..5526)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5912..5931
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6000..6599
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6000..6019)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6496..6516
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6666..7254
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6719..6739)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7137..7148)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7329..7562
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7681..7699)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7767..7871
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7872..7940,7941..8732)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8090..8109)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
       61 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      721 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      781 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      841 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      901 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      961 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     1021 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     1081 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
     1141 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1201 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     1261 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     1321 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     1381 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     1441 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     1501 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     1561 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     1621 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     1681 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     1741 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     1801 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     1861 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     1921 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     1981 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2041 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     2101 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     2161 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     2221 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     2281 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     2341 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     2401 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     2461 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     2521 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     2581 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     2641 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     2701 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     2761 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     2821 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     2881 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     2941 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     3001 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     3061 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     3121 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     3181 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     3241 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     3301 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     3361 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
     3421 cgaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     3481 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     3541 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     3601 tttggactag tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3661 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3721 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3781 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3841 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3901 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3961 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     4021 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     4081 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt
     4141 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
     4201 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     4261 agcctccgga ctctagcgtt taaacgctag ccaccatggc gtacccatac gatgttccag
     4321 attacgctgc tcgaggttat caaacaagtt tgtacaaaaa agttggcatg gcgtcccgca
     4381 aggaaggtac cggctctact gccacctctt ccagctccac cgccggcgca gcagggaaag
     4441 gcaaaggcaa aggcggctcg ggagattcag ccgtgaagca agtgcagata gatggccttg
     4501 tggtattaaa gataatcaaa cattatcaag aagaaggaca aggaactgaa gttgttcaag
     4561 gagtgctttt gggtctggtt gtagaagatc ggcttgaaat taccaactgc tttcctttcc
     4621 ctcagcacac agaggatgat gctgactttg atgaagtcca atatcagatg gaaatgatgc
     4681 ggagccttcg ccatgtaaac attgatcatc ttcacgtggg ctggtatcag tccacatact
     4741 atggctcatt cgttacccgg gcactcctgg actctcagtt tagttaccag catgccattg
     4801 aagaatctgt cgttctcatt tatgatccca taaaaactgc ccaaggatct ctctcactaa
     4861 aggcatacag actgactcct aaactgatgg aagtttgtaa agaaaaggat ttttcccctg
     4921 aagcattgaa aaaagcaaat atcacctttg agtacatgtt tgaagaagtg ccgattgtaa
     4981 ttaaaaattc acatctgatc aatgtcctaa tgtgggaact tgaaaagaag tcagctgttg
     5041 cagataaaca tgaattgctc agccttgcca gcagcaatca tttggggaag aatctacagt
     5101 tgctgatgga cagagtggat gaaatgagcc aagatatagt taaatacaac acatacatga
     5161 ggaatactag taaacaacag cagcagaaac atcagtatca gcagcgtcgc cagcaggaga
     5221 atatgcagcg ccagagccga ggagaacccc cgctccctga ggaggacctg tccaaactct
     5281 tcaaaccacc acagccgcct gccaggatgg actcgctgct cattgcaggc cagataaaca
     5341 cttactgcca gaacatcaag gagttcactg cccaaaactt aggcaagctc ttcatggccc
     5401 aggctcttca agaatacaac aactacccaa ctttcttgta caaagtggtt cgataacgaa
     5461 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     5521 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     5581 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     5641 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     5701 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     5761 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     5821 ggaaggggtg ggtccggggg caggtccggg ggcgggctca ggggcgggct caggggcggg
     5881 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     5941 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     6001 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     6061 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     6121 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6181 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6241 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6301 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6361 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6421 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6481 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6541 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     6601 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgatag
     6661 atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6721 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6781 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6841 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6901 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6961 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7021 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7081 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7141 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7201 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga
     7261 tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7321 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     7381 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7441 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7501 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7561 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7621 agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg
     7681 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7741 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7801 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7861 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7921 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7981 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8041 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8101 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8161 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8221 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8281 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8341 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8401 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8461 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8521 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8581 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8641 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8701 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8761 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8821 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8881 ta
//
