LOCUS       sequence-194473-        9563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EME1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7140 pHAGE-P-CMVt-N-HA-GAW-EME1
FEATURES             Location/Qualifiers
     source          1..9563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      16..604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     505..524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     758..775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1015..1036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1051..1056,1057..1074,1075..1081)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1089..1105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1094..1116
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1113..1129
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1113..1129
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1179..1198
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1390..2023
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2070..2195
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2692..2925
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3110..3154
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3447..3564
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3616..3919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3920..4123
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4074..4094
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4125..4143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4146..4164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4174..4198
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4285..4294
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4297..4323
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4339..4363
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6113..6137)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6156..6655
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6191..6214)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6588..6607
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6676..7275
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6676..6695)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7172..7192
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7342..7930
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7395..7415)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7813..7824)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8005..8238
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8357..8375)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8443..8547
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8548..8616,8617..9408)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8766..8785)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
       61 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      121 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
      181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      661 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      781 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      841 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
      901 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
      961 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     1021 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     1081 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     1141 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     1201 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     1261 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     1321 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     1381 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     1441 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     1501 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     1561 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     1621 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     1681 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     1741 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     1801 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     1861 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1921 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1981 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     2041 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     2101 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     2161 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     2221 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     2281 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     2341 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     2401 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     2461 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     2521 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     2581 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     2641 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     2701 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     2761 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     2821 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     2881 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     2941 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3001 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3061 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3121 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     3181 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3241 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3301 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3361 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
     3421 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     3481 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     3541 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     3601 actagtggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     3661 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     3721 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     3781 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     3841 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     3901 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     3961 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     4021 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     4081 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
     4141 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc
     4201 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
     4261 ccggactcta gcgtttaaac gctagccacc atggcgtacc catacgatgt tccagattac
     4321 gctgctcgag gttatcaaac aagtttgtac aaaaaagttg gcatggctct aaagaagtca
     4381 tcaccctcac tggattctgg tgatagtgac tctgaggagt tgccaacatt tgcctttctg
     4441 aagaaggaac catcttcaac aaagaggaga cagcctgaaa gggaagagaa gattgtagtg
     4501 gttgacatct cagattgtga agcctcctgt cctccagcac cagagttatt ttcaccacct
     4561 gtcccagaca tagctgaaac tgtcacacaa acacagccag tcaggttgct aagcagtgaa
     4621 agtgaagatg aagaagaatt tattcctctg gctcaaaggc ttacatgtaa gtttctgacc
     4681 cacaagcaac tgagccctga ggactctagc tccccagtta aaagtgtttt ggatcatcaa
     4741 aataatgaag gtgcatcatg tgactggaaa aagccctttc caaagatccc tgaagttccc
     4801 ctccatgata ccccagagag gagtgcagca gataacaagg acctgatctt agatccatgc
     4861 tgtcagcttc cagcctacct gtctacctgc cctggccaga gcagcagctt ggcagtaacc
     4921 aaaacaaatt ctgacatcct tccaccccag aagaaaacca agccgagtca gaaggtccag
     4981 ggaagaggct cacacggatg ccggcagcag agacaagcaa ggcagaagga aagcaccctg
     5041 agaagacagg aaagaaagaa tgcagcactg gttaccagga tgaaagccca gaggccagag
     5101 gaatgcttaa aacacatcat tgtagtgctg gatccagtgc tcttacagat ggaaggtggg
     5161 ggccagctcc taggagcact gcagaccatg gagtgccgct gtgtgattga ggcgcaggct
     5221 gtgccttgca gtgtcacttg gaggagaagg gctgggccgt ctgaggacag agaggactgg
     5281 gtggaggagc caacagtact ggtgttgctc cgggcagagg catttgtgtc catgatcgac
     5341 aatggaaagc agggaagcct ggacagcact atgaaaggga aggaaacgct tcagggcttt
     5401 gtaactgaca tcacagcaaa gacagcaggg aaagctctgt cactggtgat tgtggatcag
     5461 gagaaatgct tcagcctgga gctgctgttc tttgatttcc tcccctgcac cagtgctcag
     5521 aatcctccaa gaagagggaa acagggagca aataaacaga ccaagaagca gcagcagaga
     5581 caaccagagg ccagcatagg gtccatggta tccagggtag acgctgaaga ggcattggtg
     5641 gatctgcagc tacacacaga agcccaggct caaattgtgc agagctggaa agagctggcc
     5701 gacttcacat gcgcattcac aaaggctgtg gctgaggcgc ccttcaagaa gctccgagat
     5761 gaaactacct tctccttctg tctggagagt gactgggctg gaggggtgaa ggtggacctt
     5821 gctggcaggg gactcgcact agtctggagg agacagattc agcagctgaa ccgagtcagc
     5881 ctggaaatgg ccagtgcagt tgtgaatgcc tatccctccc cacagctcct ggtacaggct
     5941 tatcagcagt gtttttcgga taaagaacgc cagaatttgc tcgcagacat acaggtgcgc
     6001 cgtggggaag gtgtgacatc cacttctcgc cgcattggac cagaactatc caggcgtatc
     6061 taccttcaga tgaccacttt acagccacat ctctctttag atagtgctga ctgtccaact
     6121 ttcttgtaca aagtggttcg ataacgaatt ctaccgggta ggggaggcgc ttttcccaag
     6181 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     6241 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     6301 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     6361 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     6421 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     6481 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     6541 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     6601 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     6661 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6721 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6781 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6841 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6901 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6961 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     7021 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     7081 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     7141 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7201 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7261 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     7321 ccccatgcat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     7381 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7441 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7501 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7561 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7621 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7681 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7741 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7801 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7861 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7921 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7981 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     8041 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8101 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8161 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8221 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     8281 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     8341 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     8401 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8461 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8521 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8581 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8641 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8701 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8761 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     8821 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8881 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8941 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     9001 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9061 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9121 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9181 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9241 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9301 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9361 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9421 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9481 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9541 tgagcgtcag accccgtaga aaa
//
