LOCUS       sequence-194474-        9065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FDFT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7058 pHAGE-P-CMVt-N-HA-GAW-FDFT1
FEATURES             Location/Qualifiers
     source          1..9065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     501..520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     754..771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1011..1032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1047..1052,1053..1070,1071..1077)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1085..1101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1090..1112
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1109..1125
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1109..1125
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1175..1194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1386..2019
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2066..2191
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2688..2921
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3106..3150
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3443..3560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3612..3915
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3916..4119
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4070..4090
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4121..4139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4142..4160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4170..4194
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4281..4290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4293..4319
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4335..4359
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5611..5635)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5654..6153
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5689..5712)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6086..6105
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6174..6773
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6174..6193)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6670..6690
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6840..7428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6893..6913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7311..7322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7503..7736
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7855..7873)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7941..8045
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8046..8114,8115..8906)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8264..8283)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
       61 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      781 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
      841 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      901 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
      961 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     1021 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     1081 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     1141 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1201 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     1261 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     1321 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     1381 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     1441 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     1501 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     1561 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     1621 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     1681 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     1741 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     1801 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     1861 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1921 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1981 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     2041 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     2101 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     2161 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     2221 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     2281 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     2341 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     2401 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     2461 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     2521 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     2581 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     2641 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     2701 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     2761 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     2821 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2881 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2941 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     3001 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     3061 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     3121 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     3181 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     3241 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     3301 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     3361 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattcac
     3421 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     3481 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     3541 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggacta
     3601 gtggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3661 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3721 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3781 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3841 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     3901 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     3961 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4021 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     4081 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat
     4141 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
     4201 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
     4261 actctagcgt ttaaacgcta gccaccatgg cgtacccata cgatgttcca gattacgctg
     4321 ctcgaggtta tcaaacaagt ttgtacaaaa aagttggcat ggagttcgtg aaatgcctcg
     4381 gccaccccga agagttctac aacctggtgc gcttccggat cgggggcaag cggaaggtga
     4441 tgcccaagat ggaccaggac tcgctcagca gcagcctgaa aacttgctac aggtatctca
     4501 atcagaccag tcgcagtttc gcagctgtta tccaggcgct ggatggggaa atgcgcaacg
     4561 cagtgtgcat attttatctg gttctccgag ctctggacac actggaagat gacatgacca
     4621 tcagtgtgga aaagaaggtc ccgctgttac acaactttca ctctttcctt taccaaccag
     4681 actggcggtt catggagagc aaggagaagg atcgccaggt gctggaggac ttcccaacga
     4741 tctcccttga gtttagaaat ctggctgaga aataccaaac agtgattgcc gacatttgcc
     4801 ggagaatggg cattgggatg gcagagtttt tggataagca tgtgacctct gaacaggagt
     4861 gggacaagta ctgccactat gttgctgggc tggtcggaat tggcctttcc cgtcttttct
     4921 cagcctcaga gtttgaagac cccttagttg gtgaagatac agaacgtgcc aactctatgg
     4981 gcctgtttct gcagaaaaca aacatcatcc gtgactatct ggaagaccag caaggaggaa
     5041 gagagttctg gcctcaagag gtttggagca ggtatgttaa gaagttaggg gattttgcta
     5101 agccggagaa tattgacttg gccgtgcagt gcctgaatga acttataacc aatgcactgc
     5161 accacatccc agatgtcatc acctaccttt cgagactcag aaaccagagt gtgtttaact
     5221 tctgtgctat tccacaggtg atggccattg ccactttggc tgcctgttat aataaccagc
     5281 aggtgttcaa aggggcagtg aagattcgga aagggcaagc agtgaccctg atgatggatg
     5341 ccaccaatat gccagctgtc aaagccatca tatatcagta tatggaagag atttatcata
     5401 gaatccccga ctcagaccca tcttctagca aaacaaggca gatcatctcc accatccgga
     5461 cgcagaatct tcccaactgt cagctgattt cccgaagcca ctactccccc atctacctgt
     5521 cgtttgtcat gcttttggct gccctgagct ggcagtacct gaccactctc tcccaggtaa
     5581 cagaagacta tgttcagact ggagaacact gcccaacttt cttgtacaaa gtggttcgat
     5641 aacgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     5701 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     5761 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     5821 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     5881 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     5941 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     6001 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     6061 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     6121 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg
     6181 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     6241 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     6301 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     6361 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6421 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6481 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     6541 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     6601 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     6661 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     6721 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     6781 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atagatccta
     6841 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6901 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6961 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     7021 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     7081 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     7141 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     7201 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     7261 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     7321 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     7381 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt
     7441 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7501 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     7561 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7621 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7681 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7741 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7801 tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg
     7861 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     7921 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7981 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8041 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8101 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8161 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8221 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8281 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8341 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8401 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8461 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8521 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8581 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8641 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8701 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8761 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8821 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8881 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8941 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9001 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9061 agatc
//
