LOCUS       sequence-194476-       16113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE
            and tHMG1 of the beta-carotene pathway, each flanked by loxPsym 
            sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16113)
  AUTHORS   Hochrein L, Mitchell LA, Schulz K, Messerschmidt K, Mueller-Roeber B
  TITLE     L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination
            in yeast.
  JOURNAL   Nat Commun. 2018 May 22;9(1):1931. doi: 10.1038/s41467-017-02208-6.
  PUBMED    29789561
REFERENCE   2  (bases 1 to 16113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-22"; volume: "9(1):1931. doi"; pages: " 
            10.1038/s41467-017-02208-6"
COMMENT     Sequence Label: pLM494
FEATURES             Location/Qualifiers
     source          1..16113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..45
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     145..164
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        188..403
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             404..1207
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1338..1793)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1475..1496)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1687..1706
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1919..1941
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1933..1950
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1934..1950
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1960..1979
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1960..1978
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2003..2019
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2004..2020
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2055..2088
                     /label=loxPsym
                     /bound_moiety="Cre recombinase"
                     /note="loxPsym has a symmetric spacer region, and loxPsym 
                     sites are capable of recombining in either orientation 
                     (Hoess et al., 1986)."
     CDS             3192..3203
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    4057..4090
                     /label=loxPsym
                     /bound_moiety="Cre recombinase"
                     /note="loxPsym has a symmetric spacer region, and loxPsym 
                     sites are capable of recombining in either orientation 
                     (Hoess et al., 1986)."
     protein_bind    6658..6691
                     /label=loxPsym
                     /bound_moiety="Cre recombinase"
                     /note="loxPsym has a symmetric spacer region, and loxPsym 
                     sites are capable of recombining in either orientation 
                     (Hoess et al., 1986)."
     promoter        6927..7214
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     7084..7107
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     protein_bind    9494..9527
                     /label=loxPsym
                     /bound_moiety="Cre recombinase"
                     /note="loxPsym has a symmetric spacer region, and loxPsym 
                     sites are capable of recombining in either orientation 
                     (Hoess et al., 1986)."
     CDS             10956..10967
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    11816..11849
                     /label=loxPsym
                     /bound_moiety="Cre recombinase"
                     /note="loxPsym has a symmetric spacer region, and loxPsym 
                     sites are capable of recombining in either orientation 
                     (Hoess et al., 1986)."
     gene            11913..13269
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        11913..12256
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             12257..13066
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12324..12343)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      13072..13269
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(13341..13357)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13341..13357)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13394..13414)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13394..13412)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13433..13449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13433..13449)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13446..13468)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13457..13473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13481..13487,13488..13505,13506..13511))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13526..13547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13664..13681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13835..14423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13915..13934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14594..15385,15386..15454))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15217..15236
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15455..15559)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    15596..16099
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt gtcacagctt
       61 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
      121 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
      181 ccaccttttc aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa
      241 tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag
      301 attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac
      361 ccaactgcac agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata
      421 taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca
      481 cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga
      541 gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac
      601 tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt
      661 tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc
      721 tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg
      781 cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg
      841 ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa
      901 gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag
      961 agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt
     1021 agatgacaag ggagatgcat tgggtcaaca gtatagaacc gtggatgatg tggtgtctac
     1081 aggatctgac attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt
     1141 agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca
     1201 aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc
     1261 aatttaatta tatcagttat taccctatgc ggtgtgaaat accgcacaga tgcgtaagga
     1321 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     1381 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     1441 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     1501 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     1561 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     1621 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     1681 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     1741 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
     1801 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     1861 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     1921 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     1981 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
     2041 ctgcagcccc cagtataact tcgtataatg tacattatac gaagttatta ttagcggcgc
     2101 cgggaaatcc agcatattct cgcggccctg agcagtaggt gtctcgggca gtcaatattc
     2161 ccaaaaagag catcagacga tctggttatg gtttttcttg actataacct taattatgag
     2221 actaatgtct tcgggaggtc ccttttccga ttttccgact cttttccgtt gaagaatgta
     2281 cttgtggttt tgaatcctac ggcagttatt gcggcggttt ggccctttct ttcaaagatt
     2341 gtgatggaaa taattgattg ttccgggaaa tgtgtcttat tttctaaaag catctttttt
     2401 tctctccaat tcttcgagct atttccagta aaggaaaaaa aaggtttgct gtaagggtga
     2461 atatgtctcc aacctctttg aggtactgcg ttgcttcatt caccatttaa tataaatagt
     2521 acattggcag ccctctttca aacgtcaatt attctcgctt gcctaacttt gttcggaccg
     2581 aaattataaa ggcattcaat cagtaacaat aattgctatt gcataactat accctctgct
     2641 aaataaaata aaaatggatt acgcgaacat cctcacagca attccactcg agtttactcc
     2701 tcaggatgat atcgtgctcc ttgaaccgta tcactaccta ggaaagaacc ctggaaaaga
     2761 aattcgatca caactcatcg aggctttcaa ctattggttg gatgtcaaga aggaggatct
     2821 cgaggtcatc cagaacgttg ttggcatgct acataccgct agcttattaa tggacgatgt
     2881 ggaggattca tcggtcctca ggcgtgggtc gcctgtggcc catctaattt acgggattcc
     2941 gcagacaata aacactgcaa actacgtcta ctttctggct tatcaagaga tcttcaagct
     3001 tcgcccaaca ccgataccca tgcctgtaat tcctccttca tctgcttcgc ttcaatcatc
     3061 agtctcctct gcatcctcct cctcctcggc ctcgtctgaa aacgggggca cgtcaactcc
     3121 taattcgcag attccgttct cgaaagatac gtatcttgat aaagtgatca cagacgagat
     3181 actttccctc catagagggc agggcctgga gctattctgg agagatagtc tgacgtgtcc
     3241 tagcgaagag gaatatgtga aaatggttct tggaaagacg ggaggtttgt tccgtatagc
     3301 ggtcagattg atgatggcaa agtcagaatg tgacatagac tttgtccagc ttgtcaactt
     3361 gatctcaata tacttccaga tcagggatga ctatatgaac cttcagtctt ctgagtatgc
     3421 ccataataag aattttgcag aggacctcac agaagggaaa ttcagttttc ccactatcca
     3481 ctcgattcat gccaacccct catcgagact cgtcatcaat acgttgcaga agaaatcgac
     3541 ctctcctgag atccttcacc actgtgtaaa ctacatgcgc acagaaaccc actcattcga
     3601 atatactcag gaagtcctca acaccttgtc aggtgcactc gagagagaac taggaaggct
     3661 tcaaggagag ttcgcagaag ctaactcaag gatggatctt ggtgacgtag attcggaagg
     3721 aagaacgggg aagaacgtca aattggaagc gatcctgaaa aagctagccg atatccctct
     3781 gtgatgagac ttaaatgaga aaaatttcgt aatgagataa aatttcgctc cttttctgtt
     3841 ttctattttc tattttccca acttttgctc tattcagtta taaattacta tttatccatc
     3901 agttaaaaaa caagatcttt tactggtcag ctaggaaagc gaaaatacaa agactttatg
     3961 cacttagtga tatatatgta tagatatatt ttggaggtac tggcctagcg tcgtggcccg
     4021 ggagagacag tttagtagtg actcgcggcc accctgataa cttcgtataa tgtacattat
     4081 acgaagttat tgaactcgca tgtctggggt tgtggtcagg ccttgtcaat tcagttgttt
     4141 gcaaaaagaa caaaactgaa aaaacccaga cacgctcgac ttcctgtctt cctattgatt
     4201 gcagcttcca atttcgtcac acaacaaggt cctagcgacg gctcacaggt tttgtaacaa
     4261 gcaatcgaag gttctggaat ggcgggaaag ggtttagtac cacatgctat gatgcccact
     4321 gtgatctcca gagcaaagtt cgttcgatcg tactgttact ctctctcttt caaacagaat
     4381 tgtccgaatc gtgtgacaac aacagcctgt tctcacacac tcttttcttc taaccaaggg
     4441 ggtggtttag tttagtagaa cctcgtgaaa cttacattta catatatata aacttgcata
     4501 aattggtcaa tgcaagaaat acatatttgg tcttttctaa ttcgtagttt ttcaagttct
     4561 tagatgcttt ctttttctct tttttacaga tcatcaagga agtaattatc tactttttac
     4621 aacaaatata aaacaaatgg gaaaagaaca agatcaggat aaacccacag ctatcatcgt
     4681 gggatgtggt atcggtggaa tcgccactgc cgctcgtctt gctaaagaag gtttccaggt
     4741 cacggtgttc gagaagaacg actactccgg aggtcgatgc tctttaatcg agcgagatgg
     4801 ttatcgattc gatcaggggc ccagtttgct gctcttgcca gatctcttca agcagacatt
     4861 cgaagatttg ggggagaaga tggaagattg ggtcgatctc atcaagtgtg aacccaacta
     4921 tgtttgccac ttccacgatg aagagacttt cactttttca accgacatgg cgttgctcaa
     4981 gcgggaagtc gagcgttttg aaggcaaaga tggatttgat cggttcttgt cgtttatcca
     5041 agaagcccac agacattacg agcttgctgt cgttcacgtc ctgcagaaga acttccctgg
     5101 cttcgcagca ttcttacggc tacagttcat tggccaaatc ctggctcttc accccttcga
     5161 gtctatctgg acaagagttt gtcgatattt caagaccgac agattacgaa gagtcttctc
     5221 gtttgcagtg atgtacatgg gtcaaagccc atacagtgcg cccggaacat attccttgct
     5281 ccaatacacc gaattgaccg agggcatctg gtatccgaga ggaggctttt ggcaggttcc
     5341 taatactctt cttcagatcg tcaagcgcaa caatccctca gccaagttca atttcaacgc
     5401 tccagtttcc caggttcttc tctctcctgc caaggaccga gcgactggtg ttcgacttga
     5461 atccggcgag gaacatcacg ccgatgttgt gattgtcaat gctgacctcg tttacgcctc
     5521 cgagcacttg attcctgacg atgccagaaa caagattggc caactgggtg aagtcaagag
     5581 aagttggtgg gctgacttag ttggtggaaa gaagctcaag ggaagttgca gtagtttgag
     5641 cttctactgg agcatggacc gaatcgtgga cggtctgggc ggacacaata tcttcttggc
     5701 cgaggacttc aagggatcat tcgacacaat cttcgaggag ttgggactcc cagccgatcc
     5761 ttccttttac gtgaacgttc cctcgcgaat cgatccttct gccgctcccg aaggcaaaga
     5821 tgctatcgtc attcttgtgc cgtgtggcca tatcgacgct tcgaaccctc aagattacaa
     5881 caagcttgtt gctcgggcaa ggaagtttgt gatccaaacg ctttccgcca agcttggact
     5941 tcccgacttt gaaaaaatga ttgtggcaga gaaggttcac gatgctccct cttgggagaa
     6001 agaatttaac ctcaaggacg gaagcatctt gggactggct cacaacttta tgcaagttct
     6061 tggtttcagg ccgagcacca gacatcccaa gtatgacaag ttgttctttg tcggggcttc
     6121 gactcatccc ggaactgggg ttcccatcgt cttggctgga gccaagttaa ctgccaacca
     6181 agttctcgaa tcctttgacc gatccccagc tccagatccc aatatgtcac tctccgtacc
     6241 atatggaaaa cctctcaaat caaatggaac gggtatcgat tctcaggtcc agctgaagtt
     6301 catggatttg gagagatggg tatacctttt ggtgttgttg attggggccg tgatcgctcg
     6361 atccgttggt gttcttgctt tctgatgaga gaatatgtat aaagttcatt tgttctttta
     6421 tgtatcttct agttattggt catgtttaaa atggtaaaaa taaagaaaat aatttctata
     6481 ttttagtgta taatataatc gtcatagatt tcgaagtaat gaaagaaagg ttatcattag
     6541 gtactataaa ctatgctttc tgtttgctgc gaaaatacat atgtacagtt tttttggcgt
     6601 taattgtagc ttatttcccg ccctgtgatt gaggcgggat ggtgtcccca gggaatgata
     6661 acttcgtata atgtacatta tacgaagtta tttagaacgg ccaccgtcct cagtacagtt
     6721 tattcctggc atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa
     6781 aaagaatccc agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc
     6841 tcttagcgca actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat
     6901 ggagtgatgc aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat
     6961 ctatctcatt ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga
     7021 aaaaaaaggt tgaaaccagt tccctgaaat tattccccta cttgactaat aagtatataa
     7081 agacggtagg tattgattgt aattctgtaa atctatttct taaacttctt aaattctact
     7141 tttatagtta gtcttttttt tagttttaaa acaccaagaa cttagtttcg aataaacaca
     7201 cataaacaaa caaaaatgac ggctctcgca tattaccaga tccatctgat ctatactctc
     7261 ccaattcttg gtcttctcgg cctgctcact tccccgattt tgacaaaatt tgacatctac
     7321 aaaatatcga tcctcgtatt tattgcgttt agtgcaacca caccatggga ctcatggatc
     7381 atcagaaatg gcgcatggac atatccatca gcggagagtg gccaaggcgt gtttggaacg
     7441 tttctagatg ttccatatga agagtacgct ttctttgtca ttcaaaccgt aatcaccggc
     7501 ttggtctacg tcttggcaac taggcacctt ctcccatctc tcgcgcttcc caagactaga
     7561 tcgtccgccc tttctctcgc gctcaaggcg ctcatccctc tgcccattat ctacctattt
     7621 accgctcacc ccagcccatc gcccgacccg ctcgtgacag atcactactt ctacatgcgg
     7681 gcactctcct tactcatcac cccacctacc atgctcttgg cagcattatc aggcgaatat
     7741 gctttcgatt ggaaaagtgg ccgagcaaag tcaactattg caacaatcat gatcccgacg
     7801 gtgtatctga tttgggtaga ttatgttgct gtcggtcaag actcttggtc gatcaacgat
     7861 gagaagattg tagggtggag gcttggaggt gtactaccca ttgaggaagc tatgttcttc
     7921 ttactgacga atctaatgat tgttctgggt ctgtctgcct gcgatcatac tcaggcccta
     7981 tacctgctac acggtcgaac tatttatggc aacaaaaaga tgccatcttc atttcccctc
     8041 attacaccgc ctgtgctctc cctgtttttt agcagccgac catactcttc tcagccaaaa
     8101 cgtgacttgg aactggcagt caagttgttg gagaaaaaga gccggagctt ttttgttgcc
     8161 tcggctggat ttcctagcga agttagggag aggctggttg gactatacgc attctgccgg
     8221 gtgactgatg atcttatcga ctctcctgaa gtatcttcca acccgcatgc cacaattgac
     8281 atggtatccg attttcttac cctactattt gggcccccgc tacacccttc gcaacctgac
     8341 aagatccttt cttcgccttt acttcctcct tcgcaccctt cccgacccac gggaatgtat
     8401 cccctcccgc ctcctccttc gctctcgcct gccgagctcg ttcaattcct taccgaaagg
     8461 gttcccgttc aataccattt cgccttcagg ttgctcgcta agttgcaagg gctgatccct
     8521 cgatacccac tcgacgaact ccttagagga tacaccactg atcttatctt tcccttatcg
     8581 acagaggcag tccaggctcg gaagacgcct atcgagacaa cagctgactt gctggactat
     8641 ggtctatgtg tagcaggctc agtcgccgag ctattggtct atgtctcttg ggcaagtgca
     8701 ccaagtcagg tccctgccac catagaagaa agagaagctg tgttagtggc aagccgagag
     8761 atgggaactg cccttcagtt ggtgaacatt gctagggaca ttaaagggga cgcaacagaa
     8821 gggagatttt acctaccact ctcattcttt ggtcttcggg atgaatcaaa gcttgcgatc
     8881 ccgactgatt ggacggaacc tcggcctcaa gatttcgaca aactcctcag tctatctcct
     8941 tcgtccacat taccatcttc aaacgcctca gaaagcttcc ggttcgaatg gaagacgtac
     9001 tcgcttccat tagtcgccta cgcagaggat cttgccaaac attcttataa gggaattgac
     9061 cgacttccta ccgaggttca agcgggaatg cgagcggctt gcgcgagcta cctactgatc
     9121 ggccgagaga tcaaagtcgt ttggaaagga gatgtcggag agagaaggac agttgccgga
     9181 tggaggagag tacggaaagt cttgagtgtg gtcatgagcg gatgggaagg gcagtgatga
     9241 gaggaaaatt tgatttttga ttcagggtat gcgactattc aaacacgtat tttcatatac
     9301 gaatgagtag tttataattt atataaatat atattacata aggaaatggc tatgacttca
     9361 tgatttttac gctttgagtc ccctatgcct accggttatg ttatatactt ggcaaatgat
     9421 atcaatgaat agaaaacaaa atttttgcac ggcgctaggt gtgatatcgt acacttggga
     9481 gaagtcagat gagataactt cgtataatgt acattatacg aagttataac tctccgcagc
     9541 acctccggac tatgcccgag tggtcagtgt taaacgtgtg gtttatgggt gcaccagggc
     9601 tttatcgtgt tttatatcga tggcgatttg tgcctccagt gtatttttgt atatccaatt
     9661 aaggtttctt acctaatttt atttttatca tctttagtta atgctggttt gctctgtttc
     9721 tgctgctttc tgtgcggttc tcctcttctc ttgtttcttc gtgttgtccc ccatcgccga
     9781 tgggcttata tggcgtatat atatagagcg agtttttacg tcgaagatca tctcagtttg
     9841 cttgatagcc tttctacttt attactttcg tttttaacct cattatactt tagttttctt
     9901 tgatcggttt ttttctctgt atacttaaaa gttcaaatca aagaaacata caaaactacg
     9961 tttatatcaa ttaataaatg gaccaattgg tgaaaactga agtcaccaag aagtctttta
    10021 ctgctcctgt acaaaaggct tctacaccag ttttaaccaa taaaacagtc atttctggat
    10081 cgaaagtcaa aagtttatca tctgcgcaat cgagctcatc aggaccttca tcatctagtg
    10141 aggaagatga ttcccgcgat attgaaagct tggataagaa aatacgtcct ttagaagaat
    10201 tagaagcatt attaagtagt ggaaatacaa aacaattgaa gaacaaagag gtcgctgcct
    10261 tggttattca cggtaagtta cctttgtacg ctttggagaa aaaattaggt gatactacga
    10321 gagcggttgc ggtacgtagg aaggctcttt caattttggc agaagctcct gtattagcat
    10381 ctgatcgttt accatataaa aattatgact acgaccgcgt atttggcgct tgttgtgaaa
    10441 atgttatagg ttacatgcct ttgcccgttg gtgttatagg ccccttggtt atcgatggta
    10501 catcttatca tataccaatg gcaactacag agggttgttt ggtagcttct gccatgcgtg
    10561 gctgtaaggc aatcaatgct ggcggtggtg caacaactgt tttaactaag gatggtatga
    10621 caagaggccc agtagtccgt ttcccaactt tgaaaagatc tggtgcctgt aagatatggt
    10681 tagactcaga agagggacaa aacgcaatta aaaaagcttt taactctaca tcaagatttg
    10741 cacgtctgca acatattcaa acttgtctag caggagattt actcttcatg agatttagaa
    10801 caactactgg tgacgcaatg ggtatgaata tgatttctaa aggtgtcgaa tactcattaa
    10861 agcaaatggt agaagagtat ggctgggaag atatggaggt tgtctccgtt tctggtaact
    10921 actgtaccga caaaaaacca gctgccatca actggatcga aggtcgtggt aagagtgtcg
    10981 tcgcagaagc tactattcct ggtgatgttg tcagaaaagt gttaaaaagt gatgtttccg
    11041 cattggttga gttgaacatt gctaagaatt tggttggatc tgcaatggct gggtctgttg
    11101 gtggatttaa cgcacatgca gctaatttag tgacagctgt tttcttggca ttaggacaag
    11161 atcctgcaca aaatgttgaa agttccaact gtataacatt gatgaaagaa gtggacggtg
    11221 atttgagaat ttccgtatcc atgccatcca tcgaagtagg taccatcggt ggtggtactg
    11281 ttctagaacc acaaggtgcc atgttggact tattaggtgt aagaggcccg catgctaccg
    11341 ctcctggtac caacgcacgt caattagcaa gaatagttgc ctgtgccgtc ttggcaggtg
    11401 aattatcctt atgtgctgcc ctagcagccg gccatttggt tcaaagtcat atgacccaca
    11461 acaggaaacc tgctgaacca acaaaaccta acaatttgga cgccactgat ataaatcgtt
    11521 tgaaagatgg gtccgtcacc tgcattaaat cctgagactt aaatgagaaa aatttcgtaa
    11581 tgagataaaa tttcgctcct tttctgtttt ctattttcta ttttcccaac ttttgctcta
    11641 ttcagttata aattactatt tatccatcag ttaaaaaaca agatctttta ctggtcagct
    11701 aggaaagcga aaatacaaag actttatgca cttagtgata tatatgtata gatatatttt
    11761 tgaagctacg cgccgagcgt ctgactcctt tagtccgcgt catcgctttg aaactataac
    11821 ttcgtataat gtacattata cgaagttatg cctgatgtgg ccgtcctatt agcattgtac
    11881 accctcattc agtgccgggt cacccggcca gcgacatgga ggcccagaat accctccttg
    11941 acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac atttagccca
    12001 tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg gcgcgaagca
    12061 aaaattacgg ctcctcgctg cagacctgcg agcagggaaa cgctcccctc acagacgcgt
    12121 tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat ttgccactga
    12181 ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct cacatcacat
    12241 ccgaacataa acaaccatgg gtaaggaaaa gactcacgtt tcgaggccgc gattaaattc
    12301 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
    12361 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
    12421 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
    12481 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
    12541 caccactgcg atccccggca aaacagcatt ccaggtatta gaagaatatc ctgattcagg
    12601 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
    12661 taattgtcct tttaacagcg atcgcgtatt tcgactcgct caggcgcaat cacgaatgaa
    12721 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
    12781 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg
    12841 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
    12901 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
    12961 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
    13021 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag tactgacaat
    13081 aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt
    13141 ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca
    13201 gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc
    13261 gctatactgc tgtcgattcg atactaacgc cgccttttga ctaagactct ggtcacggtt
    13321 cagaagtgga cgattttggg gatccactag ttctagagcg gccgccaccg cggtggagct
    13381 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
    13441 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    13501 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    13561 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    13621 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    13681 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    13741 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    13801 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    13861 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    13921 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    13981 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    14041 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    14101 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    14161 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    14221 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    14281 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    14341 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    14401 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    14461 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    14521 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    14581 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    14641 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    14701 taccatctgg ccccagtgct gcaatgatac cgcgagatcc acgctcaccg gctccagatt
    14761 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    14821 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    14881 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    14941 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    15001 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    15061 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    15121 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    15181 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    15241 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    15301 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    15361 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    15421 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    15481 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    15541 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac
    15601 gtgctataaa aataattata atttaaattt tttaatataa atatataaat taaaaataga
    15661 aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct
    15721 agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg
    15781 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat
    15841 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat
    15901 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca
    15961 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa
    16021 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt
    16081 aagttacagg caagcgatcc gtctaagaaa cca
//
